Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Analysis of thousands of Δmsh-2 octads using our fluorescent recombination system indicates that, as in other filamentous fungi, symmetric heteroduplex is common in the his-3 region of Neurospora crassa. Symmetric heteroduplex arises from Holliday junction migration, and we suggest this mechanism explains the high frequency of His+ spores in heteroallelic crosses in which recombination is initiated cis to the his-3 allele further from the initiator, cog+. In contrast, when recombination is initiated cis to the his-3 allele closer to cog+, His+ spores are mainly a result of synthesis-dependent strand annealing, yielding asymmetric heteroduplex. Loss of Msh-2 function increases measures of allelic recombination in both his-3 and the fluorescent marker gene, indicating that mismatches in asymmetric heteroduplex, as in Saccharomyces cerevisiae, tend to be repaired in the direction of restoration. Furthermore, the presence of substantial numbers of conversion octads in crosses lacking Msh-2 function suggests that the disjunction pathway described in S. cerevisiae is also active in Neurospora, adding to evidence for a universal model for meiotic recombination

Extracting information from vehicle exteriors via soil and insect DNA

© 2017 Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ This author accepted manuscript is made available following 12 month embargo from date of publication (Oct 2017) in accordance with the publisher’s archiving policyDNA present on the surface of a vehicle provides evidence of past journeys which may be of forensic value. We show here the type of information that can be economically sourced by arbitrarily primed PCR and next generation sequencing of the DNA present in crushed insects and dust adhering to the upper surfaces and mud adhering to the underbody of a vehicle

Recombination in filamentous fungi

London, U

Sequence heterology and gene conversion at his-3 in Neurospora crassa

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DNA repair and recombination

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Initial characterisation of phytotoxic proteins isolated from Pyrenophora teres

Pyrenophora teres, the causal agent of net blotch of barley (Hordeum vulgare L.), induces distinctive long dark brown lesions (the net form) or dark brown spots (the spot form), both with surrounding chlorosis. Proteins isolated from P. teres f. teres (the net form) and P. teres f. maculata (the spot form) induce host-specific necrosis. In this study, the activity of these proteinaceous toxins was further characterised. The toxins were heat stable and their activity in planta was temperature and light dependent suggesting a role in targeting metabolically active and light-dependent organelles such as the chloroplast. In addition, proteinase K treatment after toxin treatment did not affect symptom induction by the toxins suggesting they are actively internalised. Adult barley plants were less sensitive to the toxins than younger plants, highlighting a potential adult resistance mechanism. Characterisation of these host-specific proteinaceous toxins produced by P. teres further assists our understanding of the role of such selective toxins in disease induction in plants. © 2008 Elsevier Ltd. All rights reserved.Abolfazl Sarpeleh, Hugh Wallwork, Max E. Tate, David E.A. Catcheside and Amanda J. Abl

Diversification of exogenous genes in vivo in Neurospora

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