Genomic Features for Desiccation Tolerance and Sugar Biosynthesis in the Extremophile Gloeocapsopsis sp. UTEX B3054

For tolerating extreme desiccation, cyanobacteria are known to produce both compatible solutes at intracellular level and a copious amount of exopolysaccharides as a protective coat. However, these molecules make cyanobacterial cells refractory to a broad spectrum of cell disruption methods, hindering genome sequencing, and molecular studies. In fact, few genomes are already available from cyanobacteria from extremely desiccated environments such as deserts. In this work, we report the 5.4 Mbp draft genome (with 100% of completeness in 105 contigs) of Gloeocapsopsis sp. UTEX B3054 (subsection I; Order Chroococcales), a cultivable sugar-rich and hardly breakable hypolithic cyanobacterium from the Atacama Desert. Our in silico analyses focused on genomic features related to sugar-biosynthesis and adaptation to dryness. Among other findings, screening of Gloeocapsopsis genome revealed a unique genetic potential related to the biosynthesis and regulation of compatible solutes and polysaccharides. For instance, our findings showed for the first time a novel genomic arrangement exclusive of Chroococcaceae cyanobacteria associated with the recycling of trehalose, a compatible solute involved in desiccation tolerance. Additionally, we performed a comparative genome survey and analyses to entirely predict the highly diverse pool of glycosyltransferases enzymes, key players in polysaccharide biosynthesis and the formation of a protective coat to dryness. We expect that this work will set the fundamental genomic framework for further research on microbial tolerance to desiccation and to a wide range of other extreme environmental conditions. The study of microorganisms like Gloeocapsopsis sp. UTEX B3054 will contribute to expand our limited understanding regarding water optimization and molecular mechanisms allowing extremophiles to thrive in xeric environments such as the Atacama Desert

Differential effects of RNA-dependent RNA polymerase 6 (RDR6) silencing on new and old world begomoviruses in Nicotiana benthamiana

RNA-dependent RNA polymerases (RDRs) are key players in the antiviral defence mediated by RNA silencing in plants. RDR6 is one of the major components of the process, regulating the infection of certain RNA viruses. To better clarify its function against DNA viruses, we analyzed the effect of RDR6 inactivation (RDR6i) in N. benthamiana plants on two phloem-limited begomoviruses, the bipartite Abutilon mosaic virus (AbMV) and the monopartite tomato yellow leaf curl Sardinia virus (TYLCSV). We observed exacerbated symptoms and DNA accumulation for the New World virus AbMV in RDR6i plants, varying with the plant growth temperature (ranging from 16 °C to 33 °C). However, for the TYLCSV of Old World origin, RDR6 depletion only affected symptom expression at elevated temperatures and to a minor extent; it did not affect the viral titre. The accumulation of viral siRNA differed between the two begomoviruses, being increased in RDR6i plants infected by AbMV but decreased in those infected by TYLCSV compared to wild-type plants. In situ hybridization revealed a 6.5-fold increase in the number of AbMV-infected nuclei in RDR6i plants but without egress from the phloem tissues. These results support the concept that begomoviruses adopt different strategies to counteract plant defences and that TYLCSV evades the functions exerted by RDR6 in this host

WiNDS: An H<SUB> α </SUB> Kinematics Survey of Nearby Spiral Galaxies-Vertical Perturbations in Nearby Disk-type Galaxies

International audienceWe present the Waves in Nearby Disk galaxies Survey (WiNDS) consisting of 40 nearby low-inclination disk galaxies observed through H α high-resolution Fabry-Perot interferometry. WiNDS consists of 12 new galaxy observations and 28 data archived observations obtained from different galaxy surveys. We derive two-dimensional line-of-sight velocity fields that are analyzed to identify the possible presence of vertical velocity flows in the galactic disks of these low-inclination late-type galaxies using velocity residual maps, derived from the subtraction of an axisymmetric rotation model from a rotational velocity map. Large and globally coherent flows in the line-of-sight velocity of nearly face-on galaxies can be associated with large vertical displacement of the disk with respect to its midplane. Our goal is to characterize how frequent vertical perturbations, such as those observed in the Milky Way, arise in the local universe. Our currently available data have allowed us to identify 20% of WiNDS galaxies with strong velocity perturbations that are consistent with vertically perturbed galactic disks

Differential Effects of RNA-Dependent RNA Polymerase 6 (RDR6) Silencing on New and Old World Begomoviruses in <i>Nicotiana benthamiana</i>

RNA-dependent RNA polymerases (RDRs) are key players in the antiviral defence mediated by RNA silencing in plants. RDR6 is one of the major components of the process, regulating the infection of certain RNA viruses. To better clarify its function against DNA viruses, we analyzed the effect of RDR6 inactivation (RDR6i) in N. benthamiana plants on two phloem-limited begomoviruses, the bipartite Abutilon mosaic virus (AbMV) and the monopartite tomato yellow leaf curl Sardinia virus (TYLCSV). We observed exacerbated symptoms and DNA accumulation for the New World virus AbMV in RDR6i plants, varying with the plant growth temperature (ranging from 16 °C to 33 °C). However, for the TYLCSV of Old World origin, RDR6 depletion only affected symptom expression at elevated temperatures and to a minor extent; it did not affect the viral titre. The accumulation of viral siRNA differed between the two begomoviruses, being increased in RDR6i plants infected by AbMV but decreased in those infected by TYLCSV compared to wild-type plants. In situ hybridization revealed a 6.5-fold increase in the number of AbMV-infected nuclei in RDR6i plants but without egress from the phloem tissues. These results support the concept that begomoviruses adopt different strategies to counteract plant defences and that TYLCSV evades the functions exerted by RDR6 in this host

A Tidally Induced Global Corrugation Pattern in an External Disk Galaxy Similar to the Milky Way

We study the two-dimensional (2D) line-of-sight velocity (Vlos) field of the low-inclination, late-type galaxy VV304a. The resulting 2D kinematic map reveals a global, coherent, and extended perturbation that is likely associated with a recent interaction with the massive companion VV304b. We use multiband imaging and a suite of test-particle simulations to quantify the plausible strength of in-plane flows due to nonaxisymmetric perturbations and show that the observed velocity flows are much too large to be driven either by a spiral structure or by a bar. We use fully cosmological hydrodynamical simulations to characterize the contribution from in- and off-plane velocity flows to the Vlos field of recently interacting galaxy pairs like the VV304 system. We show that, for recently perturbed low-inclination galactic disks, the structure of the residual velocity field, after subtraction of an axisymmetric rotation model, can be dominated by vertical flows. Our results indicate that the Vlos perturbations in VV304a are consistent with a corrugation pattern. Its Vlos map suggests the presence of a structure similar to the Monoceros ring seen in the Milky Way. Our study highlights the possibility of addressing important questions regarding the nature and origin of vertical perturbations by measuring the line-of-sight velocities in low-inclination nearby galaxies