Displaced Voices: A Journal of Migration, Archives and Cultural Heritage, Volume 3 Issue 2 (Autumn 2023)

Twentieth Century Histories of Civic Society’s Responses to Crises of Displacement: A Special Issue to mark the 70th Anniversary of Refugee Council Displaced Voices is a biannual digital magazine produced twice a year by the Living Refugee Archive team at the University of East London. Displaced Voices aims to provide a digital platform for activists, archivists, researchers, practitioners and academics to contribute to issues pertaining to refugee and migration history; refugee and migrant rights; social justice; cultural heritage and archives. We welcome a range of contributions to the magazine including articles of between 1000-2000 words; reports on fieldwork in archival collections; book recommendations and reviews; and more creative pieces including (but not limited too) cartoons; photography; and poetry. We would also welcome news on activities; publication of reports, projects; letters and news from your own networks. We welcome submissions from all writers whether you are a student, practitioner, activist or established academic. The Displaced Voices online magazine is born out of the collaborative and intersectional work that we have been undertaking through our work with the refugee and migration archives housed at the University of East London. Our work to date has explored the intersections of refugee and migration studies with narrative and life history research linked to oral history methods and archival approaches to the preservation, documentation and accessibility of archival resources recording the refugee experience. This magazine is a collaborative project between the Living Refugee Archive at the University of East London; the Oral History Society Migration Special Interest Group and the International Association for the Study of Forced Migration Working Group on the History of Forced Migration and Refugees. Thematically we are looking to engage with articles that explore the intersection of refugee and forced migration studies; history and cultural heritage studies; narrative research; oral history and archival science

Edificio de oficinas, en Madrid

This office building was designed according to a module of 1.60 X 1.60 m, to allow for maximum flexibility in its distribution and optimum adaptation to possible future changes in the internal organisation. The structure is metallic; the external walls are in brick or glass, depending on the zone, and both the internal and external treatment have been wisely chosen in order to follow the two basic principles of functionality and comfort. This building, besides containing the main offices of the Spanish Society of Touring Cars in Madrid, also provides room for a lecture hall, an automobile exhibition hall and a department of electronic computing.<br><br>Este edificio para oficinas fue proyectado según un módulo de 1,60 x 1,60 m, de tal manera que admitiese la máxima flexibilidad en su distribución y se adaptase a todos los cambios de organización que pudieran presentarse en el futuro. La estructura es metálica; los cerramientos, de ladrillo visto y cristal, según conviene a cada zona; y en su composición y tratamientos exterior e interior se han aunado sabiamente los dos principios básicos de representatividad y confort exigidos. Además de alojar las oficinas centrales de la Sociedad Española de Automóviles de Turismo en Madrid, como elementos singulares dispone de: salón de actos, exposición de automóviles y servicio de ordenador electrónico

Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data

Abstract Background Genetically engineered mice (GEM) are essential tools for understanding gene function and disease modeling. Historically, gene targeting was first done in embryonic stem cells (ESCs) derived from the 129 family of inbred strains, leading to a mixed background or congenic mice when crossed with C57BL/6 mice. Depending on the number of backcrosses and breeding strategies, genomic segments from 129-derived ESCs can be introgressed into the C57BL/6 genome, establishing a unique genetic makeup that needs characterization in order to obtain valid conclusions from experiments using GEM lines. Currently, SNP genotyping is used to detect the extent of 129-derived ESC genome introgression into C57BL/6 recipients; however, it fails to detect novel/rare variants. Results Here, we present a computational pipeline implemented in the Galaxy platform and in BASH/R script to determine genetic introgression of GEM using next generation sequencing data (NGS), such as whole genome sequencing (WGS), whole exome sequencing (WES) and RNA-Seq. The pipeline includes strategies to uncover variants linked to a targeted locus, genome-wide variant visualization, and the identification of potential modifier genes. Although these methods apply to congenic mice, they can also be used to describe variants fixed by genetic drift. As a proof of principle, we analyzed publicly available RNA-Seq data from five congenic knockout (KO) lines and our own RNA-Seq data from the Sall2 KO line. Additionally, we performed target validation using several genetics approaches. Conclusions We revealed the impact of the 129-derived ESC genome introgression on gene expression, predicted potential modifier genes, and identified potential phenotypic interference in KO lines. Our results demonstrate that our new approach is an effective method to determine genetic introgression of GEM

Respuesta de navicula rhynchocephala (bacillariophyceae) al incremento de nutrientes en el río tota

RESUMEN   Con el fin de evaluar el efecto de la adición de nutrientes sobre la estructura de las comunidades de diatomeas en el río Tota, se cuantificó la densidad de diatomeas (células cm-2) sobre sustratos artificiales (cerámicas) en dos estaciones ubicadas a lo largo del río Tota (control e impacto). Las estaciones estudiadas presentaron diferencias significativas en la disponibilidad de nutrientes y las condiciones medio ambientales permanecieron similares en cada estación de muestreo. La única especie que presentó diferencias significativas con relación al incremento de nutrientes fue Navicula rhynchocephala (n=52, F= 4,657; p= 0,036), por lo cual se considera una especie indicadora debido a su respuesta al incremento de nutrientes.     ABSTRACT   In order to evaluate the effect of  nutrients addition on the structure of diatoms communities in the Tota stream, diatoms density was quantified (cells cm-2) on artificial substrates (ceramic) in two stations located along the Tota stream (control and impact). The studied stations showed significant differences in nutrients availability whereas the environmental conditions were similar in each station of sampling. Navicula rhynchocephala showed significant differences in relation to the increment of nutrients (n=52, F = 4,657; p = 0,036), reason for which it is considered an indicative species, due to its response to the increment of nutrients

Effects of dexmedetomidine and esmolol on systemic hemodynamics and exogenous lactate clearance in early experimental septic shock

Persistent hyperlactatemia during septic shock is multifactorial. Hypoperfusion-related anaerobic production and adrenergic-driven aerobic generation together with impaired lactate clearance have been implicated. An excessive adrenergic response could contribute to persistent hyperlactatemia and adrenergic modulation might be beneficial. We assessed the effects of dexmedetomidine and esmolol on hemodynamics, lactate generation, and exogenous lactate clearance during endotoxin-induced septic shock. Methods: Eighteen anesthetized and mechanically ventilated sheep were subjected to a multimodal hemodynamic/perfusion assessment including hepatic and portal vein catheterizations, total hepatic blood flow, and muscle microdialysis. After monitoring, all received a bolus and continuous infusion of endotoxin. After 1 h they were volume resuscitated, and then randomized to endotoxin-control, endotoxin-dexmedetomidine (sequential doses of 0.5 and 1.0 μg/k/h) or endotoxin-esmolol (titrated to decrease basal heart rate by 20 %) groups. Samples were taken at four time points, and exogenous lactate clearance using an intravenous administration of sodium L-lactate (1 mmol/kg) was performed at the end of the experiment