Quistes de Tarlov sintomáticos: diagnóstico y tratamiento

Los quistes de Tarlov se forman a expensas de la duramadre y de la aracnoides alrededor de las raíces sacras o coccígeas. Habitualmente asintomáticos, en ocasiones pueden ocasionar clínica álgica de irritación radicular. El desarrollo de técnicas de imagen, principalmente la resonancia magnética nuclear (RMN), permiten diagnosticarlos con más frecuencia, aunque determinar si son responsables de los síntomas de los pacientes continúa siendo difícil. El tratamiento inicial es conservador mediante analgésicos convencionales y fisioterapia. En caso de quistes sintomáticos de gran tamaño, sin respuesta al tratamiento inicial, puede ser necesario el tratamiento quirúrgico mediante drenaje percutáneo guiado bajo control de la tomografía axial computerizada (TAC), o bien mediante una técnica abierta que permita la eliminación del quiste y la descompresión del nervio. Describimos dos casos clínicos en pacientes con clínica de neuralgia por atrapamiento de las raíces nerviosas lumbosacras, un paciente tratado con infiltración periradicular y otro que requirió un drenaje percutáneo con buen resultado final.Tarlov cysts are formed of the dura mater and the arachnoid around the sacral or coccygeal roots. Usually asymptomatic, they can occasionally cause clinical radicular irritation. Magnetic resonance imaging (MRI) permit to diagnose them more often, but whether they are responsible for the symptoms remains difficult. The initial treatment is conservative with conventional analgesics and physiotherapy. In case of large symptomatic cysts, with no response to initial treatment may require surgical therapy: a guided percutaneous drainage under computerized tomography (CT) control, or by an open technique that allows removal of the cyst and nerve decompression. We describe two cases in patients with symptomatic neuralgia due to entrapment of lumbosacral nerve roots. One patient treated with periradicular infiltration and another that required percutaneous drainage with good final outcome

Gestational hypothyroxinemia affects its offspring with a reduced suppressive capacity impairing the outcome of the experimental autoimmune encephalomyelitis

Indexación: Scopus.Hypothyroxinemia (Hpx) is a thyroid hormone deficiency (THD) condition highly frequent during pregnancy, which although asymptomatic for the mother, it can impair the cognitive function of the offspring. Previous studies have shown that maternal hypothyroidism increases the severity of experimental autoimmune encephalomyelitis (EAE), an autoimmune disease model for multiple sclerosis (MS). Here, we analyzed the immune response after EAE induction in the adult offspring gestated in Hpx. Mice gestated in Hpx showed an early appearance of EAE symptoms and the increase of all parameters of the disease such as: the pathological score, spinal cord demyelination, and immune cell infiltration in comparison to the adult offspring gestated in euthyroidism. Isolated CD4+CD25+ T cells from spleen of the offspring gestated in Hpx that suffer EAE showed reduced capacity to suppress proliferation of effector T cells (TEff) after being stimulated with anti-CD3 and anti-CD28 antibodies. Moreover, adoptive transfer experiments of CD4+CD25+ T cells from the offspring gestated in Hpx suffering EAE to mice that were induced with EAE showed that the receptor mice suffer more intense EAE pathological score. Even though, no significant differences were detected in the frequency of Treg cells and IL-10 content in the blood, spleen, and brain between mice gestated in Hpx or euthyroidism, T cells CD4+CD25+ from spleen have reduced capacity to differentiate in vitro to Treg and to produce IL-10. Thus, our data support the notion that maternal Hpx can imprint the immune response of the offspring suffering EAE probably due to a reduced capacity to trigger suppression. Such "imprints" on the immune system could contribute to explaining as to why adult offspring gestated in Hpx suffer earlier and more intense EAE. © 2018 Haensgen, Albornoz, Opazo, Bugueño, Jara Fernández, Binzberger, Rivero-Castillo, Venegas Salas, Simon, Cabello-Verrugio, Elorza, Kalergis, Bueno and Riedel.https://www.frontiersin.org/articles/10.3389/fimmu.2018.01257/ful

A general perspective of the characterization and quantification of nanoparticles: Imaging, spectroscopic, and separation techniques

This article gives an overview of the different techniques used to identify, characterize, and quantify engineered nanoparticles (ENPs). The state-of-the-art of the field is summarized, and the different characterization techniques have been grouped according to the information they can provide. In addition, some selected applications are highlighted for each technique. The classification of the techniques has been carried out according to the main physical and chemical properties of the nanoparticles such as morphology, size, polydispersity characteristics, structural information, and elemental composition. Microscopy techniques including optical, electron and X-ray microscopy, and separation techniques with and without hyphenated detection systems are discussed. For each of these groups, a brief description of the techniques, specific features, and concepts, as well as several examples, are described.Junta de Andalucía FQM-5974CEI-Biotic Granada CEI2013- MP-1

Assessment of metals bound to marine plankton proteins and to dissolved proteins in seawater

Studies based on laser ablation–inductively coupled plasma-mass spectrometry (LA–ICP-MS) have been performed to assess metal bound to dissolved proteins and proteins from marine plankton after two-dimensional polyacrylamide gel electrophoresis (2D PAGE). Dissolved proteins were pre-concentrated from surface seawater (60 L) by tangential ultrafiltration with 10 kDa molecular weight cut-off (MWCO) membranes and further centrifugal ultrafiltration (10 kDa) before proteins isolation by methanol/chloroform/water precipitation. Proteins isolation from plankton was assessed after different trichloroacetic acid (TCA)/acetone and methanol washing stages, and further proteins extraction with a phenol solution. LA–ICP-MS analysis of the electrophoretic profiles obtained for dissolved proteins shows the presence of Cd, Cr, Cu, and Zn in five spots analyzed. These proteins exhibit quite similar molecular weights (within the 10–14 kDa range) and pIs (from 5.8 to 7.3). Cd, Cr, Cu, and Zn have also been found to be associated to proteins isolated from plankton samples. In this case, Cd has been found to be bound to proteins of quite different molecular weight (9, 13 and 22 kDa) and pIs (4.5, 5.2, 5.5, and 10). However, trace elements such as Cr, Cu and Zn appear to be mainly bound to plankton proteins of low molecular weight and variable pI

Distribución de metales pesados en sedimentos de las marismas del Odiel (Huelva, España)

Se ha analizado la distribución y contenido total de metales pesados (Co, Cu, Fe, Mn, Ni y Pb) en sedimentos de las Marismas del Odiel (SO España). Las concentraciones de estos elementos, obtenidas por espectrofotometría de absorción atómica, fueron muy elevadas para la mayoría de los elementos analizados. Su distribución no es homogénea, ni presenta un patrón geográfico marcadamente definido, pudiéndose encontrar altos niveles repartidos por toda la marisma. Existe cierto gradiente topográfico, con mayores concentraciones en puntos de menor cota. Los puntos de muestreo más aislados de la incidencia mareal y los más expuestos a mar abierto registraron los niveles más bajos.Atomic absorption spectroscopy has permitted the determination of the content of heavy metals and their distribution in sediments of the Odiel Saltmarhes (SWSpain). Avery high metal concentration has been found in most of the samples investigated but their distribution is non-homogeneous and this prevents establishing a geographical pattern. Nevertheless, a certain topographic gradient can be observed: lower levels present the highest concentrations whereas areas unaccessible by tide fluctuations or those exposed to the sea present the lowest index of contamination

Derecho a la educación: conocer para defender

En el marco del proyecto de investigación se administró una entrevista voluntaria y anónima a estudiantes de segundo año de las carreras licenciatura en Trabajo Social y Tecnicatura en Acompañamiento Terapéutico con el propósito de conocer y comprender las experiencias educativas transitadas en la universidad en tiempos de educación remota y la educación presencial en este período pos pandemia en el que la universidad habilitó las actividades presenciales.Tales experiencias se interpretaron en clave de garantización o vulneración del derecho a la educación.Universidad Nacional de La Plat

Conceptualización del transporte sostenible desde el comportamiento prosocial

Este documento informa de la necesidad de aumentar la eficacia de las políticas de transporte sostenible sobre el cambio de actitudes para el uso de diferentes modos de transporte a través de medidas de comportamiento prosocial, que pueden generar obligaciones morales a tomar parte en actividades prosociales mediante relaciones ocasionales incluidas en el modelo de activación de la norma (NAM, Norm Activation Model). La movilidad y el transporte sostenible aparecen como objetivo prioritario de intervención por parte de las instituciones nacionales e internacionales. Las medidas adoptadas tienen que ir acompañadas, para ser efectivas y producir un cambio de comportamiento hacia el transporte público, de campañas que influyan en actitudes prosociales. La intención de reducir el uso del automóvil es explicada principalmente por variables relacionadas con la moralidad y normas personales, así como las actitudes hacia el sistema de transporte (público o privado) más empleado están muy ligadas a las experiencias individuales. La aplicación del modelo de activación de la norma (NAM, Norm Activation Model) para una estrategia de transporte y movilidad sostenible, representa la activación del comportamiento prosocial para producir cambios de comportamiento de viaje de los ciudadanos y determinar una correlación entre políticas coherentes con las medidas prosociales adoptadas.Toledo-Castillo, F.; Hernandez-San-Miguel, J.; Lloret-Catalá, M. (2012). Conceptualización del transporte sostenible desde el comportamiento prosocial. Plataforma Tecnológica Española de la Carretera (PTC). http://hdl.handle.net/10251/10316

Calibration and validation of the Mini-fiber EC image analysis instrument mean fiber diameter through direct or primary measurements

The Minifiber EC (MFEC) is a portable instrument for measuring the diameter of animal fibers. Its accuracy and precision have been estimated but by comparing its measurements with those of laboratory devices that had been calibrated on other devices in turn, not on a direct or primary measure of diameter. This work attempts to test direct measurements by gravimetry, Vernier mini caliper, microscope and the classic microprojector, using a non-deformable, high resistance synthetic fiber (Kevlar) for direct measurement. The MFEC instrument is calibrated with each mean fiber diameter obtained in direct measurements and its results are compared. The conclusions drawn are that it is possible to calibrate the MFEC instrument with direct measurements on Kevlar and measurement accuracy or tolerance of 0.28 microns is obtained. This indicates a very low biased mean fiber diameter measurement by MFEC

Complications in Using Real-World Data to Study the Health of People Who Use Drugs

For the past two decades, the United States has suffered dramatic increases in a fatal drug overdose. Overdoses represent one major health concern facing people who use drugs, in a milieu including injection drug use-related infections. Accordingly, the use of real-world data to study drug-related harms, such as overdoses and injection drug-related infections, has become more common in the medical literature. Real-world data sources include electronic health records and administrative claims that can be leveraged to understand health needs and longitudinal health utilization patterns in specific groups of patients, including people who use drugs. Researchers developed various algorithms that use combinations of drug-related diagnosis codes for substance use disorders and overdoses. However, limited attention to date has been paid to the methodologic and ethical implications arising from the limitations of these algorithms

Characterization of the biochemical and nutritional content in simple white grain maize (Zea mays L.) crosses

Our research focused on the characterization of the nutritional and biochemical content of 25 genotypes: 5 S4 inbred lines and the 20 possible single crosses formed between them, due to the lack of knowledge about the nutritional composition and its variation within the germplasm of the white corn breeding program. In kernels of each genotype we determined the content of oil, starch, protein, ash and phytic acid. Results showed that for the parents and the crosses, correspondingly, the intervals of the substances under study were: a) Oil: from 5.99 to 3.84 and 6.40 to 3.55 g ? 100 g-1, b) Protein:  from 8.26 to 5.43 and 9.83 to 5.56 g ? 100 g-1, c) Starch: from 88.25 to 74.48 and 96.64 to 72.57 g ? 100 g-1, d) Ash: from 1.90 to 1.20 and 2.0 to 0.89 g ? 100 g-1, e) Phytic acid: from 2.40 to 1.08 and 2.29 to 1.11 g ? 100 g-1. We observed that in comparison to the parents, the crosses only were significantly superior in the content of starch; although, within each group there were statistical differences of the contents. The crosses that showed higher contents for a nutritional component were those in which at least one parent had a high composition of the nutrient. The variation in the nutritional and biochemical content showed that there is diversity among genotypes, which is linked to the contrasting genetic origin of the inbred lines and is feasible to be used. This research showed the potential of taking advantage of the nutritional components of white corn through crosses (specially its starch content).E Objective: To characterize the nutritional and biochemical content of 25 genotypes: 5 S4 inbred lines and the 20 possible single crosses formed between them. Design/Methodology/Approach: The content of oil, starch, protein, ash and phytic acid was determined in kernels of each genotype, using the American Association of Cereal Chemists’ methods; the information obtained was analyzed by a complete randomized experimental design and Tukey’s means tests. Results: For the parents and the crosses, correspondingly, the intervals of the substances under study were: a) Oil: from 5.99 to 3.84 and 6.40 to 3.55 g ∙ 100 g-1, b) Protein:  from 8.26 to 5.43 and 9.83 to 5.56 g ∙ 100 g-1, c) Starch: from 88.25 to 74.48 and 96.64 to 72.57 g ∙ 100 g-1, d) Ash: from 1.90 to 1.20 and 2.0 to 0.89 g ∙ 100 g-1, e) Phytic acid: from 2.40 to 1.08 and 2.29 to 1.11 g ∙ 100 g-1. Study Limitations/Implications: The study shows that in comparison to the parents, the crosses were only significantly superior in the content of starch, although there were statistical differences of the contents within each group. The crosses that showed higher contents for a nutritional component were those in which at least one parent had a high composition of the nutrient. Findings/Conclusions: The variation in the nutritional and biochemical content showed that there is diversity among genotypes, which is linked to the contrasting genetic origin of the inbred lines and is feasible to be used. This research showed the potential of taking advantage of the nutritional components of white maize through crosses (specially its starch content)