Integrating traditional field methods with emerging digital techniques for enhanced outcrop analysis of deep water channel-fill deposits

The development of emerging digital technologies that allow the collection and analysis of field data represents a significant innovation in field-based geological studies. The integration of these digital techniques with traditional sedimentological field methods facilitates considerable improvements in outcrop characterization. An example of this integrated modern approach for geological data collection is employed for the detailed characterization of a turbidite channel-lobe system of the Gorgoglione Flysch Formation in Southern Italy. The study area, exposed above the village of Castelmezzano, has been measured and described in detailed stratigraphic sections, providing data for both sedimentological analysis and correlation of the stratigraphy. In order to gain a complete perspective on the exposure and stratigraphic elements, analysis of physical outcrop data was enhanced by the use of high-resolution Gigapixel imagery and 3D photogrammetric outcrop reconstructions. The Santa Maria section has been assessed in terms of vertical and lateral facies stacking arrangements and subdivided into two component facies associations separated by a prominent concave-up erosional boundary. The lower facies association, interpreted as a frontal lobe complex, consists of tabular, thick-bedded coarse sandstones interbedded with persistent heterolithic packages of thin-bedded sandstones and mudstones, and minor soft-sediment deformed strata. The upper facies association represents the infill of a channel-form and consists of a basal conglomerate, passing gradually upwards into massive amalgamated sandstones overlain by large-scale cross-laminated sandstones. The excellent exposure of the Santa Maria section records the complete evolution of a channel-lobe system, transitioning from frontal lobe deposition through channel incision and bypass, to progressive backfilling. This study shows how facies characterization, stratigraphic correlations and reconstruction of the depositional architectures have been substantially enhanced by the use of emerging digital techniques for geological data collection

Neuroblastoma: oncogenic mechanisms and therapeutic exploitation of necroptosis

Neuroblastoma (NB) is the most common extracranial childhood tumor classified in five stages (1, 2, 3, 4 and 4S), two of which (3 and 4) identify chemotherapy-resistant, highly aggressive disease. High-risk NB frequently displays MYCN amplification, mutations in ALK and ATRX, and genomic rearrangements in TERT genes. These NB subtypes are also characterized by reduced susceptibility to programmed cell death induced by chemotherapeutic drugs. The latter feature is a major cause of failure in the treatment of advanced NB patients. Thus, proper reactivation of apoptosis or of other types of programmed cell death pathways in response to treatment is relevant for the clinical management of aggressive forms of NB. In this short review, we will discuss the most relevant genomic rearrangements that define high-risk NB and the role that destabilization of p53 and p73 can have in NB aggressiveness. In addition, we will propose a strategy to stabilize p53 and p73 by using specific inhibitors of their ubiquitin-dependent degradation. Finally, we will introduce necroptosis as an alternative strategy to kill NB cells and increase tumor immunogenicity