Non-lethal PCR genotyping of single Drosophila

In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with extant methodology and, importantly, before stocks are established. This advance should significantly facilitate several of the most fundamental and routine techniques in Drosophila genetics

Absence of Gluonic Components in Axial and Tensor Mesons

A quarkonium-gluonium mixing scheme previously developed to describe the characteristic of the pseudoscalar mesons is applied to axial and tensor mesons. The parameters of the model are determined by fitting the eigenvalues of a mass matrix. The corresponding eigenvectors give the proportion of light quarks, strange quarks and glueball in each meson. However the predictions of the model for branching ratios and electromagnetic decays are incompatible with the experimental results. These results suggest the absence of gluonic components in the states of axial and tensor isosinglet mesons analyzed here.Comment: 12 page

Topological insulator particles as optically induced oscillators: towards dynamical force measurements and optical rheology

We report the first experimental study upon the optical trapping and manipulation of topological insulator (TI) particles. By virtue of the unique TI properties, which have a conducting surface and an insulating bulk, the particles present a peculiar behaviour in the presence of a single laser beam optical tweezers: they oscillate in a plane perpendicular to the direction of the laser propagation, as a result of the competition between radiation pressure and gradient forces. In other words, TI particles behave as optically induced oscillators, allowing dynamical measurements with unprecedented simplicity and purely optical control. Actually, optical rheology of soft matter interfaces and biological membranes, as well as dynamical force measurements in macromolecules and biopolymers, may be quoted as feasible possibilities for the near future.Comment: 6 pages, 5 figures. Correspondence and requests for Supplementary Material should be addressed to [email protected]

Structural and dynamical properties of a quasi-one-dimensional classical binary system

The ground state configurations and the \lq{}\lq{}normal\rq{}\rq{} mode spectra of a $quasi$-one-dimensional (Q1D) binary system of charged particles interacting through a screened Coulomb potential are presented. The minimum energy configurations were obtained analytically and independently through molecular dynamic simulations. A rich variety of ordered structures were found as a function of the screening parameter, the particle density, and the ratio between the charges of the distinct types of particles. Continuous and discontinuous structural transitions, as well as an unexpected symmetry breaking in the charge distribution are observed when the density of the system is changed. For near equal charges we found a disordered phase where a mixing of the two types of particles occurs. The phonon dispersion curves were calculated within the harmonic approximation for the one- and two-chain structures.Comment: 11 pages, 11 fig

Reply to Piper et al.: Drosophila dietary restriction—Does it hold water?

No abstract

Uso e cobertura das terras na área de entorno do reservatório da Usina Hidrelétrica de Tombos (MG).

O trabalho mapeia o uso e cobertura vegetal das terras na área de entorno do reservatório da UHE de Tombos. A metodologia utilizada combinou práticas de geoprocessamento, produtos de sensoriamento remoto, sistema de informação geográfica (SIG) e trabalhos de campo. Os resultados mostram que a maior parte da área encontra-se recoberta com pastagens, sendo 41,11% com pasto limpo e 15,10% com pasto sujo, seguidos de matas com 15,29% da área, os demais 28,4% corresponde às outras classes de uso

The (1+(λ,λ))(1+(\lambda,\lambda)) Genetic Algorithm for Permutations

The $(1+(\lambda,\lambda))$ genetic algorithm is a bright example of an evolutionary algorithm which was developed based on the insights from theoretical findings. This algorithm uses crossover, and it was shown to asymptotically outperform all mutation-based evolutionary algorithms even on simple problems like OneMax. Subsequently it was studied on a number of other problems, but all of these were pseudo-Boolean. We aim at improving this situation by proposing an adaptation of the $(1+(\lambda,\lambda))$ genetic algorithm to permutation-based problems. Such an adaptation is required, because permutations are noticeably different from bit strings in some key aspects, such as the number of possible mutations and their mutual dependence. We also present the first runtime analysis of this algorithm on a permutation-based problem called Ham whose properties resemble those of OneMax. On this problem, where the simple mutation-based algorithms have the running time of $\Theta(n^2 \log n)$ for problem size $n$, the $(1+(\lambda,\lambda))$ genetic algorithm finds the optimum in $O(n^2)$ fitness queries. We augment this analysis with experiments, which show that this algorithm is also fast in practice.Comment: This contribution is a slightly extended version of the paper accepted to the GECCO 2020 workshop on permutation-based problem

A time of flight method to measure the speed of sound using a stereo sound card

We present an inexpensive apparatus for measuring the speed of sound, with a time of flight method, using a computer with a stereo sound board. Students measure the speed of sound by timing the delay between the arrivals of a pulse to two microphones placed at different distances from the source. It can serve as a very effective demonstration, providing a quick measurement of the speed of sound in air; we have used it with great success in Open Days in our Department. It can also be used for a full fledged laboratory determination of the speed of sound in air.Comment: Accepted for publication in The Physics Teache