İnsan kolorektal adenokarsinom hücrelerindeki Ca2+ sinyal iletimi üzerine ekonazolün etkisi

Introduction: Econazole, an azole compound widely used as an antifungal drug, is currently investigated for additional therapeutic effects. In fact, the antitumoral properties of econazole have been recently demonstrated, both in vivo and at the cellular level. However, the precise mechanism of action behind its effects is still unclear. Aim: To examine the effect of econazole on intracellular Ca2+ signaling pathways in a human adenocarcinoma cell line. Methods: [Ca2+]i was measured in Fura 2-loaded HT29 cells by fluorescence. Fluorescence was recorded from 1ml aliquots of magnetically stirred HT29 cells using a Cary Eclipse Spectrophotometer (Varian Ltd., Madrid, Spain) with excitation wavelengths of 340 and 380 nm and emission at 510 nm. Results: We have demonstrated that econazole has a dual effect on Ca2+ homeostasis. It caused a dose-dependent increase in [Ca2+]i. Both mobilization from thapsigargin-sensitive stores and extracellular Ca2+ influx contributed to the effect. A phospholipase C-dependent pathway proved to be activated by econazole. In addition, econazole greatly inhibited a physiological activation of store-operated Ca2+ entry. Conclusion: This study provides further molecular mechanism of econazole suggesting that this drug may modify the physiology of human adenocarcinoma HT29 cell line.Junta de Castilla y Leon (BU001A09), M.E.C. (fellowship C. Carrillo

Papel del ácido oleico en el sistema inmune: mecanismos de acción: revisión científica

Introduction:Although n-3 polyunsaturated fatty acids have been widely described as antiinflammatory fats, little is known about the role of oleic acid in immune system. Aim: The aim of the present review is to join all the reports available in order to analyze where exactly the knowledge concerning this topic is and what the causes of the controversial data could be. Methods: We searched electronic databases and bibliographies of selected articles were inspected for further reference. Results:Diets rich in oleic acid have beneficial effects in inflammatory-related diseases. In addition, a wide range of studies evaluate the effect of oleic acid in different cellular functions thus reporting a potential mechanism for the biological effect of such a fat. However, some controversial data can be found in literature, maybe related to the kind of study or even the dose of the reagent added. Conclusion:In conclusion, oleic acid could be reported as an anti-inflammatory fatty acid playing a role in the activation of different pathways of immune competent cells

Efecto antitumoral del ácido oleico; mecanismos de acción: revisión científica

Introduction: The beneficial effects of oleic acid in cancer processes can no longer be doubted, but little is known about the mechanisms of action behind this phenomenon. Aim: The aim of the present review is to clarify whether oleic acid has an effect on important mechanisms related to the carcinogenic processes. Aim: The aim of the present review is to clarify whether oleic acid has an effect on important mechanisms related to the carcinogenic processes. Methods:We searched electronic databases and biblio - graphies of selected articles were inspected for further reference. We focused our research on two cellular transformations characterizing cancer development: proliferation and cell death or apoptosis. Results: Numerous studies have reported an inhibition in cell proliferation induced by oleic acid in different tumor cell lines. Herein, oleic acid could suppress the over-expression of HER2 (erbB-2), a well-characterized oncogene which plays a key role in the etiology, invasive progression and metastasis in several human cancers. In addition, oleic acid could play a role in intracellular calcium signaling pathways linked to the proliferation event. Regarding cell death, oleic acid has been shown to induce apoptosis in carcinoma cells. The mechanisms behind the apoptotic event induced by oleic acid could be related to an increase in intracellular ROS production or caspase 3 activity. Several unsaturated fatty acids have been reported to induce apoptosis through a release of calcium from intracellular stores. However, evidence regarding such a role in oleic acid is lacking. Conclusions:Oleic acid plays a role in the activation of different intracellular pathways involved in carcinoma cell development. Such a role could be the root of its antitumoral effects reported in clinical studies

Barracks from modern age iberian wars (1650-1750) heritage value, contemporary use and social housing

The present paper studies 18th century military garrisons built in border villages of Portuguese Alentejo during the instability period that began with the Restoration War (1640-1668) between Portugal and Spain. Besides presenting their constructive types and materiality, the research focus on the heritage value of these barracks, especially given their urban context inside small peripheral towns’ historical centres under threat of desertification. Three cases are compared in order to determine which contemporary use better favours heritage conservation of the barracks modular structures

Nursing Students’ Perceived Satisfaction with Flipped Learning Experiences: A Mixed-Methods Study

Nowadays, technological teaching tools, such as virtual labs, have become essential, especially in nursing degrees. These resources help implement practical learning based on selfregulation and it is important to know how satisfied students are with them. This means it is important to study students’ perceived satisfaction with virtual labs in flipped learning experiences, which was the general objective of the present study. The aims of the study were: (1) to determine whether there were significant differences in nursing students’ perceived levels of satisfaction according to the type of subject or gender; (2) to ascertain what strengths and weaknesses nursing students perceived about using virtual labs; and (3) to determine the kinds of feelings (positive, negative or neutral) nursing students had using virtual labs. A mixed research methodology was applied, with a sample of 222 undergraduate nursing students at Burgos University (Spain). There were significant differences in the satisfaction perceived by students depending on the subject. Students reported medium-high satisfaction with virtual labs, although they did note that digital skills are needed to use them and suggested incorporating intelligent assistants. Virtual labs seem to be effective, although further studies are needed.This work was supported by the Vice-Rectorate for Teaching Staff from University of Burgos (Spain) (grant numbers TVLab_01, 3/02/21) and the Ministry of Science and Innovation of Spain Projects I+D+i Research Challenges (grant number PID2020-117111RB-I00, 01/01/21)

Novel Approaches for the Recovery of Natural Pigments with Potential Health Effects

The current increased industrial food production has led to a significant rise in the amount of food waste generated. These food wastes, especially fruit and vegetable byproducts, are good sources of natural pigments, such as anthocyanins, betalains, carotenoids, and chlorophylls, with both coloring and health-related properties. Therefore, recovery of natural pigments from food wastes is important for both economic and environmental reasons. Conventional methods that are used to extract natural pigments from food wastes are time-consuming, expensive, and unsustainable. In addition, natural pigments are sensitive to high temperatures and prolonged processing times that are applied during conventional treatments. In this sense, the present review provides an elucidation of the latest research on the extraction of pigments from the agri-food industry and how their consumption may improve human health

Puesta en marcha de un sistema de evaluación formativa multidisciplinar aplicado a asignaturas de Ingeniería Química y Nutrición en el Grado en Ciencia y Tecnología de los Alimentos

Ponencia presentada en: VIII Jornadas de Innovación Docente de la UBU, Burgos, 5 de abril de 2016, organizadas por el Instituto de Formación e Innovación Educativa-IFIE de la Universidad de Burgo

Architectural and intangible heritage in Andalusia: the impact of tourism after UNESCO’s inscriptions

Póster presentado en la muestra de la International Union of Architects (UIA), la UIA International Forum 2019, con sede en Bakú, bajo la temática ‘Turismo masivo en ciudades históricas'. El Consejo Superior de los Colegios de Arquitectos de España (CSCAE) seleccionó a partir de las propuestas de los Colegios de Arquitectos 6 paneles con los que participó en la exposición. Este panel ha sido desarrollado por miembros del grupo de investigación HUM700: Patrimonio y Desarrollo Urbano Territorial en Andalucía de la Universidad de Sevilla y coordinado por el catedrático y colegiado del COAS Eduardo Mosquera Adell.Poster presented at the exhibition of the International Union of Architects (UIA), the UIA International Forum 2019 in Baku, under the theme ‘Mass tourism in historical cities'. The Superior Council of the Colleges of Architects of Spain (CSCAE) selected 6 panels from the proposals of the Association of Architects. This panel has been developed by members of the Research Lab HUM700: Heritage and Urban Territorial Development in Andalusia at the University of Seville and coordinated by the professor and registered architect Eduardo Mosquera Adell.Colegio Oficial de Arquitectos de SevillaConsejo Andaluz de Colegios Oficiales de ArquitectosConsejo Superior de los Colegios de Arquitectos de Españ

Reversal of SARS-CoV2-Induced Hypoxia by Nebulized Sodium Ibuprofenate in a Compassionate Use Program

Introduction: Sodium ibuprofenate in hypertonic saline (NaIHS) administered directly to the lungs by nebulization and inhalation has antibacterial and anti-inflammatory effects, with the potential to deliver these benefits to hypoxic patients. We describe a compassionate use program that offered this therapy to hospitalized COVID-19 patients. Methods: NaIHS (50 mg ibuprofen, tid) was provided in addition to standard of care (SOC) to hospitalized COVID-19 patients until oxygen saturation levels of > 94% were achieved on ambient air. Patients wore a containment hood to diminish aerosolization. Outcome data from participating patients treated at multiple hospitals in Argentina between April 4 and October 31, 2020, are summarized. Results were compared with a retrospective contemporaneous control (CC) group of hospitalized COVID-19 patients with SOC alone during the same time frame from a subset of participating hospitals from Córdoba and Buenos Aires. Results: The evolution of 383 patients treated with SOC + NaIHS [56 on mechanical ventilation (MV) at baseline] and 195 CC (21 on MV at baseline) are summarized. At baseline, NaIHS-treated patients had basal oxygen saturation of 90.7 ± 0.2% (74.3% were on supplemental oxygen at baseline) and a basal respiratory rate of 22.7 ± 0.3 breath/min. In the CC group, basal oxygen saturation was 92.6 ± 0.4% (52.1% were on oxygen supplementation at baseline) and respiratory rate was 19.3 ± 0.3 breath/min. Despite greater pulmonary compromise at baseline in the NaIHS-treated group, the length of treatment (LOT) was 9.1 ± 0.2 gs with an average length of stay (ALOS) of 11.5 ± 0.3 days, in comparison with an ALOS of 13.3 ± 0.9 days in the CC group. In patients on MV who received NaIHS, the ALOS was lower than in the CC group. In both NaIHS-treated groups, a rapid reversal of deterioration in oxygenation and NEWS2 scores was observed acutely after initiation of NaIHS therapy. No serious adverse events were considered related to ibuprofen therapy. Mortality was lower in both NaIHS groups compared with CC groups. Conclusions: Treatment of COVID-19 pneumonitis with inhalational nebulized NaIHS was associated with rapid improvement in hypoxia and vital signs, with no serious adverse events attributed to therapy. Nebulized NaIHS s worthy of further study in randomized, placebo-controlled trials (ClinicalTrials.gov: NCT04382768).Fil: Salva, Oscar. Clínica Independencia; ArgentinaFil: Doreski, Pablo A.. Fundación Respirar; ArgentinaFil: Giler, Celia S.. Clínica Independencia; ArgentinaFil: Quinodoz, Dario C.. Sanatorio de la Cañada; ArgentinaFil: Guzmán, Lucia G.. Sanatorio de la Cañada; ArgentinaFil: Muñoz, Sonia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Carrillo, Mariana Norma del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Porta, Daniela Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Ambasch, Germán. Sanatorio Privado Mayo; ArgentinaFil: Coscia, Esteban. Sanatorio Privado Mayo; ArgentinaFil: Tambini Diaz, Jorge L.. Sanatorio Privado Mayo; ArgentinaFil: Bueno, Germán D.. Sanatorio Privado Mayo; ArgentinaFil: Fandi, Jorge O.. Clínica Independencia; ArgentinaFil: Maldonado, Miriam A.. Sanatorio San Roque; ArgentinaFil: Peña Chiappero, Leandro E.. Sanatori San Roque; ArgentinaFil: Fournier, Fernando. Clínica Francesa; ArgentinaFil: Pérez, Hernán A.. Sanatorio Alive; Argentina. University of Maryland; Estados UnidosFil: Quiroga, Mauro A.. Instituto Modelo de Cardiología; ArgentinaFil: Sala Mercado, Javier Agustin. Instituto Modelo de Cardiología; ArgentinaFil: Martínez Picco, Carlos. Clínica del Sol; ArgentinaFil: Beltrán, Marcelo Alejandro. Hospital Dr. Alberto Duhau; ArgentinaFil: Castillo Argañarás, Luis Fernando. Hospital Dr. Alberto Duhau; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ríos, Nicolás Martínez. Quimica Luar Srl; ArgentinaFil: Kalayan, Galia I.. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Beltramo, Dante Miguel. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Garcia, Nestor Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentin