Isolation of Drug-Sensitive Eimeria Species from Wild Turkey Feces and Development of a Model Bioshuttle Program for Eimeria meleagrimitis for Domestic Turkeys

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted to assess the protective efficacy of an E. meleagrimitis vaccine candidate isolated from wild turkeys with and without intermittent amprolium administration. Additionally, the impact of vaccination and/or challenge and a candidate bioshuttle program on gut permeability and the microbiome was evaluated. The E. meleagrimitis vaccine candidate induced mild disease without affecting performance. Future research must be conducted to elucidate the impact of amprolium with and without single or multi-species live coccidiosis vaccination on the microbiome and gut barrier function in turkey poults

Isolation of Drug-Sensitive Eimeria Species from Wild Turkey Feces and Development of a Model Bioshuttle Program for Eimeria meleagrimitis for Domestic Turkeys

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted to assess the protective efficacy of an E. meleagrimitis vaccine candidate isolated from wild turkeys with and without intermittent amprolium administration. Additionally, the impact of vaccination and/or challenge and a candidate bioshuttle program on gut permeability and the microbiome was evaluated. The E. meleagrimitis vaccine candidate induced mild disease without affecting performance. Future research must be conducted to elucidate the impact of amprolium with and without single or multi-species live coccidiosis vaccination on the microbiome and gut barrier function in turkey poults

Assessing the effects of a mixed Eimeria spp. challenge on performance, intestinal integrity, and the gut microbiome of broiler chickens

A mixed Eimeria spp. challenge model was designed to assess the effects of challenge on broiler chicken performance, intestinal integrity, and the gut microbiome for future use to evaluate alternative strategies for controlling coccidiosis in broiler chickens. The experimental design involved broiler chickens divided into two groups: a control group (uninfected) and a positive control group, infected with Eimeria acervulina (EA), Eimeria maxima (EM), and Eimeria tenella (ET). At day-of-hatch, 240 off-sex male broiler chicks were randomized and allocated to one of two treatment groups. The treatment groups included: (1) Non-challenged (NC, n = 5 replicate pens); and (2) challenged control (PC, n = 7 replicate pens) with 20 chickens/pen. Pen weights were recorded at d0, d16, d31, d42, and d52 to determine average body weight (BW) and (BWG). Feed intake was measured at d16, d31, d42, and d52 to calculate feed conversion ratio (FCR). Four diet phases included a starter d0–16, grower d16–31, finisher d31–42, and withdrawal d42–52 diet. At d18, chickens were orally challenged with 200 EA, 3,000 EM, and 500 ET sporulated oocysts/chicken. At d24 (6-day post-challenge) and d37 (19-day post-challenge), intestinal lesion scores were recorded. Additionally, at d24, FITC-d was used as a biomarker to evaluate intestinal permeability and ileal tissue sections were collected for histopathology and gene expression of tight junction proteins. Ileal and cecal contents were also collected to assess the impact of challenge on the microbiome. BWG and FCR from d16–31 was significantly (p < 0.05) reduced in PC compared to NC. At d24, intestinal lesion scores were markedly higher in the PC compared to the NC. Intestinal permeability was significantly increased in the PC group based on serum FITC-d levels. Cadherin 1 (CDH1), calprotectin (CALPR), and connexin 45 (Cx45) expression was also upregulated in the ileum of the PC group at d24 (6-day post-challenge) while villin 1 (VIL1) was downregulated in the ileum of the PC group. Additionally, Clostridium perfringens (ASV1) was enriched in the cecal content of the PC group. This model could be used to assess the effect of alternative coccidiosis control methods during the post-challenge with EA, EM, and ET

Development of potato lines with possible resistance to teciasolanivora and phthorimaea apercullela

IP 2213-12-298-96ARTICULO(S) EN REVISTA: One-step transformation of two Andeanpotato cultivars (Solanum tuberosum L. subsp.;andigena) / C. Trujillo, E. ... [et al.] -- En: Plant CellReports. -- Vol.20, (2001); p.637 - 641. -- ISSN;07217714

Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States

ABSTRACT: Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study

Sound Pills: Voice as a classroom innovation for equality and inclusion (podcasting, Discord, Twitter, Clubhouse)

El proyecto de innovación docente 2022-2023, titulado Píldoras sonoras: la voz como innovación en el aula para la igualdad y la inclusión (podcasting, Discord, Twitter, Clubhouse), aúna un equipo interdisciplinar de profesorado UCM y profesorado externo (de España y del extranjero), PAS, alumnado de Grado, Máster y Doctorado, una becaria de colaboración, dos becarios FPI UCM-Banco Santander y un becario FPU (doctorado). Todos sus integrantes son expertos docentes y/o investigadores en nuevas tecnologías, innovación docente y auto-aprendizaje, redes sociales, voz y podcasting. Durante la crisis de la COVID-19, se adaptaron perfectamente a la docencia virtual online y gran parte de ellos cuenta con experiencia en docencia online completa, en programas oficiales a distancia, previos al confinamiento. En este nuevo entorno, que se erige como el futuro inmediato, se ha detectado el crecimiento del uso de las redes sociales y repositorios de voz y de podcasting, frente a las redes sociales de fotografía y vídeo, que requieren de mejores dispositivos, mejor acceso Internet y en ocasiones, conocimientos avanzados de edición de vídeo y sonido. Sin embargo, la voz humana es una herramienta primordial, gratuita y accesible para todos y todas. No requiere de infraestructura, ni de logística previa y gracias a ello, elimina la brecha tecnológica por motivos de desigualdad económica, de edad, de formación y de género.Oficina para la Calidad, Vicerrectorado de Calidad, Universidad Complutense de MadridDepto. de Periodismo y Nuevos MediosFac. de Ciencias de la InformaciónFALSEsubmitte

Memorias del I Congreso Internacional de Bioingeniería y Sistemas Inteligentes de Rehabilitación - CIBSIR 2017

El I Congreso Internacional de Bioingeniería y Sistemas Inteligentes de Rehabilitación, se celebró en Quito, capital del Ecuador. Su organización estuvo a cargo de profesores e investigadores de la Escuela Politécnica Nacional (EPN), Universidad Politécnica Salesiana (UPS), Universitat Politécnica de Valencia (UPV), Universidad Técnica del Norte (UTN), Escuela Superior Politécnica del Chimborazo (ESPOCH), Universidad de las Fuerzas Armadas (ESPE), Universidad Central del Ecuador (UCE), Escuela Superior Politécnica del Litoral (ESPOL), Universidad San Francisco de Quito (USFQ), Universidad de Las Américas (UDLA) y Universidad Mariana de Colombia. Todas ellas universidades que han trabajado de manera rigurosa para la creación de un programa académico que sirva de marco para la investigación y el intercambio de conocimientos y experiencias, así como para el desarrollo de oportunidades de colaboración para promover la difusión de tecnologías relacionadas con estos campos. Este congreso fue dirigido tanto a académicos como a profesionales y estudiantes interesados en compartir conocimientos y experiencias en las áreas de Bioingeniería y Sistemas Inteligentes de Rehabilitación. En la actualidad, el creciente avance tecnológico dedicado a los campos de la Bioingeniería y Sistemas de Rehabilitación, hace necesaria la disponibilidad de un espacio de difusión para las investigaciones que se han desarrollado en instituciones de educación superior e investigación dedicadas a estos trabajos