Estresse e Burnout entre residentes multiprofissionais

OBJECTIVE: To identify associations between high-stress and burnout syndrome in multidisciplinary residents from a federal university in Rio Grande do Sul, Brazil. METHOD: This is an analytical, cross-sectional and quantitative study. A socio-demographic questionnaire, the Work Stress Scale and the Maslach Burnout Inventory-Health Services Survey (MBI-HSS) were applied to 37 residents between April and June 2011. P-valuesOBJETIVO: Identificar la asociación entre alto estrés y Burnout en residentes Multiprofesionales de una universidad federal de Rio Grande do Sul. MÉTODO: se trata de un estudio analítico, transversal, cuantitativo. Se aplicaron un formulario de datos socio-demográficos, la Escala de Estrés en el Trabajo y el Maslach Burnout Inventory- Health Services en 37 residentes entre Abril y Junio de 2011. Valores de pOBJETIVO: identificar a associação entre alto estresse e Burnout em residentes multiprofissionais de uma universidade federal do Rio Grande do Sul. MÉTODO: trata-se de estudo analítico, transversal, quantitativo. Aplicaram-se um formulário de dados socio demográficos, a Escala de Estresse no Trabalho e o Maslach Burnout Inventory-Human Services Survey (MBI-HSS) em 37 residentes entre abril e junho de 2011. Valores de

Avaliação de alguns aspectos da aquisição e desenvolvimento da linguagem de crianças nascidas pré-termo Evaluation of some aspects of the acquisition and development of language in pre-term born children

A correção da idade para avaliação motora de nascidos pré-termo tem sido consenso, o que não ocorre em outros domínios do desenvolvimento. Este estudo comparou indicadores da aquisição e desenvolvimento da linguagem, considerando-se as idades cronológica e corrigida. Foram acompanhadas por 1 a 15 meses 20 crianças hígidas nascidas entre 28 e 36 semanas (mediana 32s), com 800g a 2380g (mediana 1590g), sendo 9 adequado para a idade gestacional (AIG) e 11 pequenas para a idade gestacional (PIG). A referência de normalidade foi o roteiro de Costa et al. (1992), que contém cinco níveis de linguagem. Quanto aos comportamentos receptivos, já considerando-se a idade cronológica, houve desempenho normal em todos os níveis, exceto no nível I (0-3 meses). Em relação à linguagem expressiva, considerando-se a idade cronológica, das 50 avaliações, 6 (12%) foram normais. Com a correção da idade, em 16 avaliações (40%) as crianças adequaram-se ao nível esperado, sendo mais freqüente a adequação aos 6 e 12 meses. Considerando-se a idade cronológica, houve maior número de AIG com desempenho normal (p<0,05). Com o uso da metodologia de Costa, não foi necessário corrigir a idade para avaliação da linguagem receptiva e, para a expressiva, a freqüência maior de resultados normais nas idades corrigidas para 6 e 12 meses sugere intensificação de vigilância nestas idades.<br>The correction of the age of pre-term infants for the motor evaluation has been the accepted practice but it has not been clear in other areas. This study compared indicators of the acquisition and development of language, considering corrected and chronological ages. Twenty healthy infants born between the 28th and 36th week of gestation (median 32 weeks), weighing 800g to 2380g (median 1590g), 9 AGA and 11 SGA, were followed up to 15 months age. As a reference for normality, evaluation of Costa et al. (1992) was used, which groups predictable behavior in 5 levels. For receptive language, considering the chronological age, normal performance occurred at all levels except for Level I (0-3 months). For expressive language, considering the chronological age, 6 (12%) of the 50 evaluations showed normal performance. With their age corrected, in 16 evaluations (40%) the infants achieved the expected level, mainly at 6 and 12 months age. On the whole, for the chronological age, there was a larger number of AGA with normal performance (p<0.05). We conclude that with the use of the Costa method, it was unnecessary to correct the age for receptive language evaluation, and that, for the expressive, the high frequency of normal results at the corrected ages for 6 and 12 months, suggests that these ages constitute periods of intensification of vigilance

BFD-22 a new potential inhibitor of BRAF inhibits the metastasis of B16F10 melanoma cells and simultaneously increased the tumor immunogenicity

Benzofuroxan is an interesting ring system, which has shown a wide spectrum of biological responses against tumor cell lines. We investigated, herein, the antitumor effects of benzofuroxan derivatives (BFDs) in vitro and in a melanoma mouse model. Cytotoxic effects of twenty-two BFDs were determined by MIT assay. Effects of BFD-22 in apoptosis and cell proliferation were evaluated using Annexin V-FITC/PI and CFSE staining. In addition, the effects in the cell cycle were assessed. Flow cytometry, western blot, and fluorescence microscopy analysis were employed to investigate the apoptosis-related proteins and the BRAF signaling. Cell motility was also exploited through cell invasion and migration assays. Molecular docking approach was performed in order to verify the BFD-22 binding mode into the ATP catalytic site of BRAF kinase. Moreover, the BFD-22 antitumor effects were evaluated in a melanoma murine model using B16F10. BFD-22 was identified as a potential hit against melanoma cells. BFD-22 induced apoptosis and inhibited cell proliferation of B16F10 cells. BFD-22 has suppressed, indeed, the migratory and invasive behavior of B16F10 cells. Cyclin D1 and CDK4 expression were reduced leading to cell cycle arrest at G0/G1 phase. Of note, phosphorylation of BRAF at Ser338 was strongly down-regulated by BFD-22 in B16F10 cells. The accommodation/orientation into the binding site of BRAF was similar of BAY43-9006 (co-crystallized inhibitor of BRAF, sorafenib). Importantly, BFD-22 presented in vivo antimetastatic effects and showed better therapeutic efficacy than sorafenib and taxol. BFD-22 can be considered as a new lead compound and, then, can be helpful for the designing of novel drug candidates to treat melanoma. (C) 2016 Elsevier Inc. All rights reserved.Sao Paulo Research Foundation (FAPESP)Brazilian Governmental Agency CNPqBrazilian Governmental Agency CAPESUniv Sao Paulo, Lab Tumor Immunol, Sao Paulo, SP, BrazilButantan Inst, Biochem & Biophys Lab, Sao Paulo, SP, BrazilUniv Groningen, Univ Med Ctr Groningen, Dept Med Oncol, Hanzepl 1, Groningen, NetherlandsUniv Sao Paulo, Lab Drug Design & Dev, Sao Paulo, SP, BrazilUniv Sao Paulo, Lab Cytopathol, Dept Clin Chem & Toxicol, Fac Pharmaceut Sci, Sao Paulo, SP, BrazilButantan Inst, Genet Lab, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Expt Oncol Sect, Sao Paulo, SP, BrazilUniv Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Expt Oncol Sect, Sao Paulo, SP, BrazilFAPESP: 2009/54599-5FAPESP: 2013/07273-2FAPESP: 2013/05396-0FAPESP: 2014/07341-0FAPESP: 2014/14267-1Web of Scienc

Reply: Genetic polymorphisms and cerebrovascular disease in children with sickle cell anemia from Rio de Janeiro, Brazil

The aim of the present work was to examine possible genetic risk factors related to the occurrence of cerebrovascular disease (CVD) in Brazilian population, the frequency of βS-globin gene haplotypes and co-inheritance with α-thalassemia (-α3.7kb) and single nucleotide polymorphism of methylenetetrahydrofolate reductase (MTHFR-C677T), Factor V Leiden (FV-G1691A) and prothrombin (PT-G20210A) genes in children from Rio de Janeiro. Ninety four children with sickle cell anemia (SCA) were included, 24 patients with cerebrovascular involvement and 70 patients without CVD as control group. The mean age of children at the time of the cerebrovascular event was similar to the control group. The frequency of -α3.7kb thalassemia was similar in both groups (p=0.751). Children with Bantu/Atypical βS-globin gene haplotype presented 15 times more chance (OR=15.4 CI 95% 2.9-81.6) of CVD than the other βS-globin gene haplotypes. The C677T polymorphism of MTHFR gene was similar in both groups (p=0.085). No mutation in the FV Leiden or PT genes was found. A large study seems necessary to establish the role of these genetic polymorphisms in Brazilian miscegenated population