O impacto da automação da fenotipagem eritrocitária estendida na rotina de um serviço de hemoterapia

Objetivos. Avaliar o impacto da automação na fenotipagem eritrocitária expandida e o nível de concordância dessa com a metodologia manual em amostras de doadores de sangue atendidos no hemocentro coordenador da Fundação Hemopa no período de janeiro a dezembro de 2019. Material e Métodos. Foram analisadas 2.700 fenotipagens eritrocitárias realizadas por metodologia manual e automatizada através do equipamento IH500 da BioRad®. Os resultados foram testados quanto ao nível de concordância através do teste de Coeficiente Kappa. Resultados. Das amostras fenotipadas 98,6% (2.662/2.700) foram concordantes em ambas as metodologias e apenas 1,4% (38/2700) foram discordantes. Das 38 amostras discordantes 31,6% referiram-se ao fenótipo Lu(b); 15,8% ao fenótipo Lu(a); 13,1% ao fenótipo Fy(b); 7,9% aos fenótipos Le(b), E, c; 5,3% aos fenótipos N, S, s, Kp(a), P1; e 2,6% aos fenótipos M, Jk(a), Jk(b), Fy(a). Conclusões. O nível de concordância entre os dados obtidos através das técnicas de fenotipagem eritrocitária manual e automatizada foi de 98,6%. Já a implantação dessa metodologia teve um impacto positivo com o aumento em 1.649 amostras processadas a mais em relação ao mesmo período do ano anterior.Objective. Evaluate the impact of automation on expanded erythrocyte phenotyping and the level of agreement between it and the manual methodology in samples from blood donors treated at the blood center coordinating the Fundação HEMOPA from january to december 2019. Material and Methods. 2,700 erythrocyte phenotyping performed by manual and automated methodology using BioRad® IH500 equipment was analyzed. The results were tested for the level of agreement using the Kappa Coefficient test. Results. Of the phenotyped samples, 98,6% (2,662 / 2,700) were in agreement in both methodologies and only 1,4% (38/2700) were in disagreement. Of the 38 discordant samples, 31,6% referred to the Lu(b) phenotype; 15,8% to the Lu(a) phenotype; 13,1% to the Fy phenotype (b); 7,9% to Le(b), E, c phenotypes; 5,3% to N, S, s, Kp (a), P1 phenotypes; and 2,6% for phenotypes M, Jk(a), Jk(b), Fy(a). Conclusions. The level of agreement between data obtained through manual and automated erythrocyte phenotyping techniques was 98.6%. The implementation of this methodology had a positive impact, with an increase of 1,649 more processed samples compared to the same period of the previous year

As manifestações clínicas do portador de paralisia facial

Introdução: A paralisia facial e um sintoma de um transtorno de base resultante em imobilidade e incapacidade de executar a mímica facial e a expressão emotiva. São várias as etiologias deste acometimento, sendo a classificação em paralisia facial periférica, representada classicamente pela paralisia de Bell e a central, pelo acidente vascular encefálico, adotada para auxiliar na investigação e seguimento clínico adequado. Objetivo: Descrever sobre a paralisia facial, com foco em características clínicas que propiciem ao diagnóstico precoce, medidas terapêuticas e restauração imediata. Metodologia: Revisão narrativa que selecionou artigos disponibilizados na íntegra publicados no recorte temporal de 2008 até 2022. Resultados: Dos 10 artigos incluídos neste estudo, todos realizaram uma ampla análise sobre a temática, a qual propiciou a disseminação de informações atualizadas sobre conceito, diagnóstico diferencial entre duas condições clínicas opostas resultantes em paralisia facial, manifestações, avaliação clínica e manejo adequado. Conclusão: Estudos ainda urgem em ser feitos no intuito de orientar melhor os profissionais e a comunidade a respeito da paralisia facial e a importância que possui o acompanhamento e seguimento precoce. Destarte,

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others

Revista de logopedia, foniatría y audiología

Resumen basado en el de la publicaciónResumen en inglésSe presenta un estudio que tuvo como objetivo crear un red de aprendizaje colaborativo entre alumnos sobre la temática de educación auditiva y vocal, a través de clases presenciales y formación a distancia. El proyecto fue llevado a cabo con 31 alumnos de primaria y secundaria que recibieron la formación y el título de 'jóvenes doctores' y que después enseñaron sus conocimientos a 1750 personas (alumnos, profesores, padres y comunidad educativa en general). De esta forma se estableció una cadena productiva del saberES

Use of chromogenic medium and semi-nested PCR-based assay to identify Candida species <br> Utilização de um meio cromogênico e da técnica de semi-nested PCR para identificação de espécies de Cândida

In this study, the chromogenic medium CHROMagar Candida™ and semi-nested PCR-based assay (sn- PCR) were compared in relation to their capacity to identify the species of 52 clinical isolates of Candida sp. By using the chromogenic medium, 39 (75%) yeasts isolates were presumptively identified as C. albicans (n = 22), C. glabrata (n = 9), C. tropicalis (n = 5) and C. krusei (n = 3). Thirteen isolates (25%) were not distinguishable to the species level. Through the sn-PCR, that is based on the ribosomal RNA genes (5.85 – 28S) cluster amplifications, 43 (83%) isolates were identified as C. albicans (n = 24), C. glabrata (n = 11), C. tropicalis (n = 5), C. parapsilosis (n = 3) and nine isolates could not be identified to the species level. Among the 52 analyzed isolates, 34 (65.4%) were in accordance with both methods, 12 (23.1%) showed discrepant results, and 6 (11.5%) could not be identified by any of the methodologies. The results indicate that both methods are limited, but the sn-PCR is more adequate than the chromogenic medium to identify species of the Candida genus, due to the lowest number of isolates that could not be identified. <p><p>Nesse trabalho, o meio cromogênico CHROMagar Candida e a técnica de semi-nested PCR (sn-PCR) foram comparados quanto a sua capacidade de identificar a espécie de 52 isolados clínicos de Cândida sp. Com o emprego do meio cromogênico, 39 (75%) isolados foram identificados presuntivamente como C. albicans (n = 22), C. glabrata (n = 9), C. tropicalis (n = 5) e C. krusei (n = 3). Treze isolados (25%) não puderam ser identificados. Por meio da técnica de sn-PCR, que se baseia na amplificação de uma região do cluster gênico do RNA ribossomal (5.8S – 28S), 43 (83%) isolados foram identificados como C. albicans (n = 24), C. glabrata (n = 11), C. tropicalis (n = 5), C. parapsilosis (n = 3) e nove isolados não puderam ser identificados em nível de espécie. Entre os 52 isolados analisados, 34 (65,4%) apresentaram resultados concordantes pelos dois métodos, 12 (23,1%) apresentaram resultados discrepantes, e 6 (11,5%) não puderam ser identificadas por nenhuma das duas metodologias. Os resultados indicam que ambas as técnicas apresentam limitações, mas o método de sn-PCR é mais adequado que o meio cromogênico para a identificação de espécies do gênero Candida, devido ao menor número de isolados que não puderam ser identificados

Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

The present study proposes to legitimize in sepsis a characteristic found in platelets that suffer storage lesions in blood banks, which is the increased expression of miRNA miR-320a in relation to miR-127. Under physiologically normal conditions, an inverse relationship is observed. The aim of this study was to verify whether the analysis of miR-320a and miR-127 expression in platelets could detect a decrease in their viability and function due to the presence of pathogens in the blood of patients hospitalized in the Intensive Care Unit. We also investigated the expression of membrane antigens sensitive to platelet activation. Of the 200 patients analyzed, only those who developed sepsis (140) were found to have a higher relative quantity of miR-320a than that of miR-127. This characteristic and the increased expression of membrane antigens P2Y12, CD62P, CD41, and CD61 showed a significant association (p &lt; 0.01) with all types of sepsis evaluated in this study. Additionally, 40% of patients hospitalized for sepsis had negative results for the first cultures. We conclude that analysis of miR-127 and miR-320a expression combined with membrane antigens evaluation, in association with the available clinical and diagnostic parameters, are important tools to detect the onset of sepsis

First report of the East-Central South African 1 Genotype of Chikungunya Virus in Rio de Janeiro, Brazil

bioRxiv preprint first posted online Oct. 20, 2016. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.Submitted by Sandra Infurna ([email protected]) on 2018-02-15T14:04:36Z No. of bitstreams: 1 jessica_silva_etal_IOC_2017.pdf: 295978 bytes, checksum: 84ccd315cea4b5258dc6045f540fa0d8 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-02-15T14:04:48Z (GMT) No. of bitstreams: 1 jessica_silva_etal_IOC_2017.pdf: 295978 bytes, checksum: 84ccd315cea4b5258dc6045f540fa0d8 (MD5)Made available in DSpace on 2018-02-15T14:04:48Z (GMT). No. of bitstreams: 1 jessica_silva_etal_IOC_2017.pdf: 295978 bytes, checksum: 84ccd315cea4b5258dc6045f540fa0d8 (MD5) Previous issue date: 2017Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Universidade Federal do Estado do Rio de Janeiro. Escola de Medicina e Cirurgia. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Rio Laranjeiras Hospital. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Escola de Medicina e Cirurgia. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Rio Laranjeiras Hospital. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.Chikungunya virus (CHIKV) is an arbovirus that causes an acute febrile illness characterized by severe and debilitating arthralgia. In Brazil, the Asian and East-Central South African (ECSA) genotypes are circulating in the north and northeast of the country, respectively. In 2015, the first autochthonous cases in Rio de Janeiro, Brazil were reported but until now the circulating strains have not been characterized. Therefore, we aimed here to perform the molecular characterization and phylogenetic analysis of CHIKV strains circulating in the 2016 outbreak occurred in the municipality of Rio de Janeiro

Epidemiologia e desfecho dos pacientes de alto risco cirúrgico admitidos em unidades de terapia intensiva no Brasil

OBJECTIVE: To define the epidemiological profile and the main determinants of morbidity and mortality in noncardiac high surgical risk patients in Brazil. METHODS: This was a prospective, observational and multicenter study. All noncardiac surgical patients admitted to intensive care units, i.e., those considered high risk, within a 1-month period were evaluated and monitored daily for a maximum of 7 days in the intensive care unit to determine complications. The 28-day postoperative, intensive care unit and hospital mortality rates were evaluated. RESULTS: Twenty-nine intensive care units participated in the study. Surgeries were performed in 25,500 patients, of whom 904 (3.5%) were high-risk (95% confidence interval - 95%CI 3.3% - 3.8%) and were included in the study. Of the participating patients, 48.3% were from private intensive care units, and 51.7% were from public intensive care units. The length of stay in the intensive care unit was 2.0 (1.0 - 4.0) days, and the length of hospital stay was 9.5 (5.4 - 18.6) days. The complication rate was 29.9% (95%CI 26.4 - 33.7), and the 28-day postoperative mortality rate was 9.6% (95%CI 7.4 - 12.1). The independent risk factors for complications were the Simplified Acute Physiology Score 3 (SAPS 3; odds ratio - OR = 1.02; 95%CI 1.01 - 1.03) and Sequential Organ Failure Assessment Score (SOFA) on admission to the intensive care unit (OR = 1.17; 95%CI 1.09 - 1.25), surgical time (OR = 1.001, 95%CI 1.000 - 1.002) and emergency surgeries (OR = 1.93, 95%CI, 1.10 - 3.38). In addition, there were associations with 28-day mortality (OR = 1.032; 95%CI 1.011 - 1.052), SAPS 3 (OR = 1.041; 95%CI 1.107 - 1.279), SOFA (OR = 1.175, 95%CI 1.069 - 1.292) and emergency surgeries (OR = 2.509; 95%CI 1.040 - 6.051). CONCLUSION: Higher prognostic scores, elderly patients, longer surgical times and emergency surgeries were strongly associated with higher 28-day mortality and more complications during the intensive care unit stay