47 research outputs found
Public awareness of cancer risk factors in the Moroccan population: a population-based cross-sectional study
Mild and Moderate Extraction Methods to Assess Potentially Available Soil Organic Nitrogen
Arachnids of medical importance in Brazil: main active compounds present in scorpion and spider venoms and tick saliva
Comparing The Cost-Effectiveness of Apcc Vs Rfviia In on-Demand Treatment of Bleeds In Hemophilia A Patients With Inhibitors: A Brazilian Public Health System Perspective
Platelet-derived exosomes are redox signaling particles
Sepsis, the body’s response to infection, is associated with extremely high mortality rates. Why a protective mechanism turns into a deadly clinical picture is a matter of debate, and goes largely unexplained. In previous work we demonstrated that platelet-derived microparticles (MP) can induce endothelial and vascular smooth muscle cell apoptosis in septic patients through NADPH oxidasedependent superoxide release [1]. In this work we sought to create a model for ex vivo generation of septic-like MP and to identify the pathways responsible for MP free radical release and effects. Septic shock is a condition related to the generation of high amounts of thrombin, TNFα and nitrogen reactive species. Human platelets exposed to the NO donors diethylamine-NONOate (0.5 mM) and nitroprusside (2 mM) for 20 minutes generated MP similar to those found in the blood of septic shock patients. Flow cytometry and western blot analysis of those MP, like their septi