Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley

Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.España, MINECO AGL2014-52028-R and AGL2017-82447-

En torno a la exposición “Naturalezas Ilustradas. La Colección Van Berkhey del Museo Nacional de Ciencias Naturales” que se celebra en el MNCN.CSIC

Sección: La RedLa conocida como colección Van Berkhey, que se custodia en el Archivo del Museo Nacional de Ciencias Naturales, fue comprada por la Corona en 1785: un rey a quién le gustaba la Ciencia (https://www.museodelprado.es/coleccion/galeria-on-line/galeria-on-line/obra/carlos-iii-nino/), un Secretario de Estado y del Despacho que deseaba ilustrar a la sociedad española, un Cónsul de España en Ámsterdam polifacético y un Director de uno de los mejores Gabinetes de Historia Natural, se comprometieron en la tarea de adquirir la colección de Historia Natural del médico neerlandés Johannes Le Francq van Berkhey, una de las más famosas de Europa en su época.N

Sequentially generated states for the study of two dimensional systems

Matrix Product States can be defined as the family of quantum states that can be sequentially generated in a one-dimensional system. We introduce a new family of states which extends this definition to two dimensions. Like in Matrix Product States, expectation values of few body observables can be efficiently evaluated and, for the case of translationally invariant systems, the correlation functions decay exponentially with the distance. We show that such states are a subclass of Projected Entangled Pair States and investigate their suitability for approximating the ground states of local Hamiltonians.Comment: 10 pages, 4 figure

Sex differences in behavioral traits related with high sensitivity to the reinforcing effects of cocaine

Cocaine is the most prevalent illegal stimulant drug in Europe among the adult population. Its abuse is characterized by a faster substance abuse disorder (SUD) development than other drugs, with high vulnerability to relapse. However, there does not exist an effective treatment for cocaine dependence. Sex differences have been reported in psychological disorders including SUD. For this reason, it is essential to identify risk factors that predict susceptibility or resilience to cocaine addiction for the development of effective prevention strategies considering sex differences. In the present study, the main objective was to determine more sensitive phenotypes to the conditioned reinforcing effects of cocaine in both sexes. Anxiety-like behavior and the locomotor response to novelty were evaluated in the elevated plus maze, and despair in the tail suspension test, as well as vulnerability traits linked with a high sensitivity to the reinforcing effects of a subthreshold dose of cocaine (1 mg/kg) in the conditioned place preference (CPP) paradigm in male and female mice. Our results indicated that only female mice with high anxiety, low locomotor response to novelty or low despair levels acquired CPP induced by cocaine, while male mice with low anxiety, high locomotor response to novelty or high despair levels presented a higher susceptibility to the rewarding effects of cocaine than others. These sex differences in the results reveal an opposite pattern in males and females on the relationship between anxiety- and depressive-like behaviors and cocaine vulnerability, demonstrating the need to include female mice in preclinical studies

Electro-optical Simulation of a-Si Thin-Film-Transistor Liquid Crystal Display Pixels

An analysis of an amorphous silicon (a-Si) thin-film-transistor liquid-crystal display (TFT-LCD) pixel is presented. The electro-optical model combines the electrical properties of the switching element and the optical performance of a twisted nematic (TN) liquid-crystal cell.Publicad

Formation of S0 galaxies through mergers. Evolution in the Tully-Fisher relation since z∼1z\sim1

(Abridged version) We explore whether a scenario that combines an origin by mergers at $z\sim$1.8-1.5 with a subsequent passive evolution of the resulting S0 remnants since $z \sim$0.8-1 is compatible with observational data of S0s in the Tully-Fisher relation (TFR). We studied a set of major and minor merger experiments from the GalMer database that generate massive S0 remnants. We analysed the location of these remnants in the photometric and stellar TFRs assuming that they correspond to $z\sim0.8$ galaxies. We then estimated their evolution in these planes over the last 7 Gyr. The results were compared with data of real S0s and spirals at different redshifts. We also tested how the use of Vcirc or Vrot,max affects the results. We found that just after $\sim$1-2 Gyr of coalescence, major mergers generate S0 remnants that are outliers of the local photometric and stellar TFRs at $z\sim0.8$. After $\sim$4-7 Gyr of passive evolution in isolation, the S0 remnants move towards the local TFR, although the initial scatter among them persists. This scatter is sensitive to the indicator used for the rotation velocity: Vcirc values yield a lower scatter than when Vrot,max values are considered instead. In the planes involving Vrot,max, a clear segregation of the S0 remnants in terms of the spin-orbit coupling of the model is observed, in which the remnants of retrograde encounters overlap with local S0s hosting counter-rotating discs. The location of the S0 remnants at $z\sim 0$ agrees well with the observed distribution of local S0 galaxies in the $\sigma_0$-$M_K$, Vcirc-$\sigma_0$ and Vrot,max-$\sigma_0$ planes. Thus, massive S0 galaxies may have been formed through major mergers that occurred at high redshift and have later evolved towards the local TFR through passive evolution in relative isolation, a mechanism that would also contribute to the scatter observed in this relation.Comment: 19 pages, 15 figures. Accepted for publication in A&

Las alteraciones de la medula ósea en el perro y el gato

El estudio de la médula ósea, como órgano hématopoyético, es necesario siempre que existan anomalías persistentes o no explicables en el hemograma. En este trabajo se realiza una revisión de las diferentes alteraciones que pueden presentarse en la médula ósea señalando especialmente los detalles morfológicos más importantes para el diagnóstico de los procesos hematológicos a los que acompañan o de los que son origen