25 research outputs found

    Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

    Get PDF
    This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

    A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids - PDA Matrisome

    No full text
    LFQ analysis of matrisome fractions enriched from 12 murine PDA or PanIN samples (samples 1 - 12) and a normal pancreas pool (samples 13) pooled from 10 pancreata of C57-Bl6 animals. Three independent enrichments have been conducted for each sample with each having had four injections

    Pulldown of proteins interacting with SGOL1-AS1 in AML

    No full text
    Epigenetic dysregulation is a common feature of acute myeloid leukemia (AML). Recently it has become clear that long noncoding RNAs (lncRNAs) can play a key role in epigenetic regulation, and consequently also dysregulation. Currently, our understanding of the requirements and roles of lncRNAs in AML is still limited. Using CRISPRi screening, we identified the lncRNA SGOL1-AS1 as an essential regulator of survival in THP-1 AML cells. We use RNA affinity purification using a biotinylated bait to pull down binding partners of the lncRNA, SGOL1-AS1. The identified proteins show a signficant enrichment for chromatin-modifying proteins involved in gene repression and chromosome organization

    A KRAS-responsive long non-coding RNA controls microRNA

    No full text
    RNA Antisense Purification and Mass Spectrometry (RAP-MS) was performed to explore the potential RNA binding proteins of lncRNA KIMAT

    A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids - 3D PEG-CBF-0.5 MS

    No full text
    LC-MS/MS based analysis of murine PCOs growing in 3D PEG-CBF-0.5 hydrogels for four days

    A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids - CDM Sets

    No full text
    Cell-derived matrix (CDM) datasets from KPC-1 or HPDE cells after seven days of growth

    A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids - Pancreas matrisome atlas

    No full text
    LC-MS/MS based analysis of the normal pancreas matrisome from 10 C57-Black6 animals. Matrisome abundance was quantified using a label-free quantification approach

    Neutrophil depletion does not alter the immune infiltrate of COX-2-competent and deficient tumors

    No full text
    Data related to Figure 1 and Figure S3. Tumor weight, total leukocyte, neutrophil, NK cell, monocyte, TAM, CD11c+ MHCII+ cell and cDC1 frequencies in melanoma tumors four days after tumor cell implantation into mice depleted of GR-1+ cells

    Proteomic analysis of Rac1 signaling regulation by guanine nucleotide exchange factors

    No full text
    The small GTPase Rac1 is implicated in various cellular processes that are essential for normal cell function. Deregulation of Rac1 signaling has also been linked to a number of diseases, including cancer. The diversity of Rac1 functioning in cells is mainly attributed to its ability to bind to a multitude of downstream effectors following activation by Guanine nucleotide Exchange Factors (GEFs). Despite the identification of a large number of Rac1 binding partners, factors influencing downstream specificity are poorly defined, thus hindering the detailed understanding of both Rac1's normal and pathological functions. In a recent study, we demonstrated a role for 2 Rac-specific GEFs, Tiam1 and P-Rex1, in mediating Rac1 anti- versus pro-migratory effects, respectively. Importantly, via conducting a quantitative proteomic screen, we identified distinct changes in the Rac1 interactome following activation by either GEF, indicating that these opposing effects are mediated through GEF modulation of the Rac1 interactome. Here, we present the full list of identified Rac1 interactors together with functional annotation of the differentially regulated Rac1 binding partners. In light of this data, we also provide additional insights into known and novel signaling cascades that might account for the GEF-mediated Rac1-driven cellular effects.</p

    ANNMAP Database

    No full text
    annmap provides annotation mappings for Affymetrix exon arrays and coordinate based queries to support deep sequencing data analysis. Database access is hidden behind the API which provides a set of functions such as genesInRange(), geneToExon(), exonDetails(), etc. Functions to plot gene architecture and BAM file data are also provided. Underlying data are from Ensembl
    corecore