Caratterizzazione tassonomica e funzionale della fauna ittica nel Lago Maggiore con particolare riferimento alla competizione per le risorse alimentari e alle specie alloctone invasive di recente comaparsa

Not availableRicerche sull\u27evoluzione del Lago Maggiore. Aspetti limnologici. Programma triennale 2013-2015. Campagna 2014. Caratterizzazione tassonomica e funzionale della fauna ittica nel Lago Maggiore con particolare riferimento alla competizione per le risorse alimentari e alle specie alloctone invasive di recente comaparsa. Composizione della comunit? ittica del Lago Maggiore: abbondanze assolute e relative; distribuzione verticale; caratterizzazione della comunit? ittica. Sovrapposizione nella nicchia trofica

Caratterizzazione tassonomica e funzionale della comunit? ittica nel Lago Maggiore con particolare riferimento alle specie alloctone invasive di recente comparsa e alla sovrapposizione della nicchia trofica

Not availableRicerche sull\u27evoluzione del Lago Maggiore. Aspetti limnologici. Programma triennale 2013-2015. Campagna 2013. Caratterizzazione tassonomica e funzionale della comunit? ittica nel Lago Maggiore con particolare riferimento alle specie alloctone invasive di recente comparsa e alla sovrapposizione della nicchia trofica. Premessa. Composizione della comunit? ittica del Lago Maggiore: abbondanze assolute e relative; distribuzione verticale; caratterizzazione della comunit? ittica: considerazione conclusive. Sovrapposizione nella nicchia trofica

Protocollo di campionamento della fauna ittica nei laghi italiani

Abstract not availableIl presente documento definisce le modalit? per il campionamento della fauna ittica in ambienti lacustri finalizzato alla valutazione dello stato ecologico di un lago in linea con le richieste della Direttiva 2000/60/CE (European Union 2000). Il protocollo definisce un metodo standard per il campionamento della fauna ittica finalizzato alla raccolta dei dati necessari per valutare la composizione, abbondanze e struttura di popolazione della comunit? ittica in un ambiente lacustre. Il protocollo si basa sui documenti UNI EN di riferimento a livello Europeo (UNI EN 14757; UNI EN 14962)

Indagine sul comparto ittico. Campionamento e preparazione del campione

Not availableIndagine sul comparto ittico. Campionamento e preparazione del campion

780 Appearing and disappearing right-heart thrombus: a case reporto in COVID-19 patient

Abstract Aims Venous thromboembolism represents frequent complication of patients with severe COVID-19 disease. Several reports about atypical thrombosis are described, rarely it has been described a right venticular thrombus during the course of infection. We report a case of right endoventricular thrombosis in a patient with SARS-Cov-2 pneumonia. Methods and results A 58-year-old man was admitted to our ward for severe respiratory failure in interstitial pneumonia. The nasopharyngeal swab for COVID-19 resulted positive. Steroids and prophylaxis with LMWH were started, associated to CPAP to maintain good gas exchange. During hospitalization a venous ECD was performed with evidence of left popliteal thrombosis despite the therapy. d-Dimer was 4463  ng/ml. A new onset AF was documented at the telemetry, without troponin elevation. A cardiac ultrasound was performed showing a right endoventricular lesion of 1.8  cm adhering to the free wall of the right ventricle. A CT-pulmonary angiogram (CTPA) resulted negative for pulmonary embolism and confirmed suspected right ventricular thrombus. Treatment with fondaparinux 7.5 mg was started. After 10 days, cardiac ultrasound shown complete resolution of thrombosis, and CT confirmed the disappearing of the mass. Dabigatran 150  mg twice/day was started. Patient clinically improved and he was discharged after 20 days of hospitalization. Conclusions SARS-CoV-2 infection may cause inflammation with cytokine storm and hypercoagulability leading to venous thromboembolism. Atypical thrombus formation was reported, including right-ventricle free wall. Early caridac ultrasound was critical to make diagnosis and starting prompt treatment, therefore routine cardiac ultrasound is mandatory in severe COVID-19 patients

Cancer immunotherapy is accompanied by distinct metabolic patterns in primary and secondary lymphoid organs observed by non-invasive in vivo18F-FDG-PET

Purpose: Cancer immunotherapy depends on a systemic immune response, but the basic underlying mechanisms are still largely unknown. Despite the very successful and widespread use of checkpoint inhibitors in the clinic, the majority of cancer patients do not benefit from this type of treatment. In this translational study, we investigated whether noninvasive in vivo positron emission tomography (PET) imaging using 2-[18F]fluoro-2-deoxy-D-glucose (18F-FDG) is capable of detecting immunotherapy-associated metabolic changes in the primary and secondary lymphoid organs and whether this detection enables the prediction of a successful anti-cancer immune response. Methods: RIP1-Tag2 mice with progressed endogenous insular cell carcinomas underwent a combined cancer immunotherapy consisting of CD4+ T cells plus monoclonal antibodies (mAbs) against programmed death ligand-1 (PD-L1) and lymphocyte activation gene-3 (LAG-3) or a sham treatment after radiation-mediated immune cell depletion. A second cohort of RIP1-Tag2 mice underwent exclusive checkpoint inhibitor therapy (CIT) using anti-PD-L1/LAG-3 mAbs or sham treatment without initial immune cell depletion to mimic the clinical situation. All mice were monitored by 18F-FDG-PET combined with anatomical magnetic resonance imaging (MRI). In addition, we retrospectively analyzed PET / computed tomography (CT) scans (PET/CT) regarding 18F-FDG uptake of CIT-treated metastatic melanoma patients in the spleen (n=23) and bone marrow (BM; n=20) as well as blood parameters (n=17-21). Results: RIP1-Tag2 mice with advanced insular cell carcinomas treated with combination immunotherapy exhibited significantly increased 18F-FDG uptake in the spleen compared to sham-treated mice. Histopathology of the spleens from treated mice revealed atrophy of the white pulp with fewer germinal centers and an expanded red pulp with hyperplasia of neutrophils than those of sham-treated mice. Immunohistochemistry and flow cytometry analyses of the spleens revealed a lower number of T cells and a higher number of neutrophils compared to those in the spleens of sham-treated mice. Flow cytometry of the BM showed enhanced activation of T cells following the treatment schemes that included checkpoint inhibitors. The ratio of 18F-FDG uptake at baseline to the uptake at follow-up in the spleens of exclusively CIT-treated RIP1-Tag2 mice was significantly enhanced, but the ratio was not enhanced in the spleens of the sham-treated littermates. Flow cytometry analysis confirmed a reduced number of T cells in the spleens of exclusively CIT-treated mice compared to that of sham-treated mice. A retrospective analysis of clinical 18F-FDG-PET/CT scans revealed enhanced 18F-FDG uptake in the spleens of some successfully CIT-treated patients with metastatic melanoma, but there were no significant differences between responders and non-responders. The analysis of the BM in clinical 18F-FDG-PET/CT scans with a computational segmentation tool revealed significantly higher baseline 18F-FDG uptake in patients who responded to CIT than in non-responders, and this relationship was independent of bone metastasis, even in the baseline scan. Conclusions: Thus, we are presenting the first translational study of solid tumors focusing on the metabolic patterns of primary and secondary lymphoid organs induced by the systemic immune response after CIT. We demonstrate that the widely available 18F-FDG-PET modality is an applicable translational tool that has high potential to stratify patients at an early time point