5 research outputs found
Response Prediction in Modified Treatment of Chronic Hepatitis B
Treatment of chronic HBV infection leads to functional cure (HBsAg loss) in only a minority of patients. Over the last decades, multiple strategies aiming to optimize the effect of t
Serum hepatitis B virus RNA predicts response to peginterferon treatment in HBeAg-positive chronic hepatitis B
Hepatitis B virus (HBV) RNA in serum is a novel biomarker that reflects cccDNA activity. We investigated whether HBV RNA can predict serological response to peginterferon (PEG-IFN) treatment. Serum HBV RNA levels were retrospectively measured at weeks 0, 12, 24 and 52 of therapy and after treatment discontinuation (week 78) in 266 HBeAg-positive chronic HBV patients who had participated in a global randomized controlled trial (HBV99-01 study). Patients received 52 weeks PEG-IFN monotherapy (n = 136) or PEG-IFN and lamivudine (n = 130). The primary end point was HBeAg loss 24 weeks after PEG-IFN discontinuation. At baseline, the mean serum level of HBV RNA was 6.8 (SD 1.2) log c/mL. HBV RNA levels declined to 4.7 (1.7) log c/mL after one year of PEG-IFN therapy alone and to 3.3 (1.2)log c/mL after combination therapy. From week 12 onward, HBV RNA level was significantly lower in patients who achieved HBeAg loss at the end of follow-up as compared to those who did not, regardless of treatment allocation (week 12:4.4 vs 5.1 log c/mL, P =.01; week 24:3.7 vs 4.9 log c/mL, P <.001). The performance of a multivariable model based on HBV RNA level was comparable at week 12 (AUC 0.68) and 24 (AUC 0.72) of therapy. HBV RNA level above 5.5 log c/mL at week 12 showed negative predictive values of 93/67/90/64% for HBV genotypes A/B/C/D for the prediction of HBeAg loss. In conclusion, HBV RNA in serum declines profoundly during PEG-IFN treatment. Early on-treatment HBV RNA level may be used to predict nonresponse
Low hepatitis B surface antigen and HBV DNA levels predict response to the addition of pegylated interferon to entecavir in hepatitis B e antigen positive chronic hepatitis B
Background: Various treatment combinations of peginterferon (PEGâIFN) and
nucleos(t)ide analogues have been evaluated for chronic hepatitis B (CHB), but the
optimal regimen remains unclear.
Aims: To study whether PEGâIFN addâon increases response compared to entecavir
(ETV) monotherapy, and whether the duration of ETV pretreatment influences
response.
Methods: Response was evaluated in HBeAg positive patients previously treated in
two randomized controlled trials. Patients received ETV pretreatment for at least
24 weeks and were then allocated to 24â48 weeks of ETV+PEGâIFN addâon, or
continued ETV monotherapy. Response was defined as HBeAg loss combined with
HBV DNA <200 IU/mL 48 weeks after discontinuing PEGâIFN.
Results: Of 234 patients, 118 were assigned PEGâIFN addâon and 116 continued
ETV monotherapy. Response was observed in 38/118 (33%) patients treated with
addâon therapy and in 23/116 (20%) with monotherapy (P = 0.03). The highest
response to addâon therapy compared to monotherapy was observed in PEGâIFN
naive patients with HBsAg levels below 4000 IU/mL and HBV DNA levels below
50 IU/mL at randomization (70% vs 34%; P = 0.01). Above the cutâoff levels,
response was low and not significantly different between treatment groups. Duration of ETV pretreatment was associated with HBsAg and HBV DNA levels (both
P < 0.005), but not with response (P = 0.82).
Conclusions: PEGâIFN addâon to ETV therapy was associated with higher response
compared to ETV monotherapy in patients with HBeAg positive CHB. Response
doubled in PEGâIFN naive patients with HBsAg below 4000 IU/mL and HBV DNA
below 50 IU/mL, and therefore identifies them as the best candidates for PEGâIFN
addâon (Identifiers: NCT00877760, NCT01532843)
Hepatitis B virus RNA decline without concomitant viral antigen decrease is associated with a low probability of sustained response and hepatitis B surface antigen loss
Background: Serum hepatitis B virus (HBV) RNA may reflect intrahepatic HBV replication. Novel anti-viral drugs have shown potent HBV RNA decline without concomitant hepatitis B surface antigen (HBsAg) decrease. How this relates to off-treatment response is yet unclear. Aim: To study the degree of on-treatment viral antigen decline among patients with pronounced HBV RNA decrease in relation to off-treatment sustained response and HBsAg loss. Methods: HBV RNA, HBsAg and hepatitis B core-related antigen (HBcrAg) were quantified in patients with chronic hepatitis B who participated in two randomised controlled trials of peginterferon-based therapy. Sustained response (HBV DNA 2 log HBV RNA decline or >1 log decline resulting in an undetectable value at on-treatment week 24), stratified by concomitant HBsAg decline (1 log). Results: We enrolled 279 patients; 176 were hepatitis B e antigen (HBeAg)-positive, and 103 were HBeAg-negative. Sustained response was achieved in 20.4% of patients. At on-treatment week 24, HBV RNA response was associated with higher
Machine-learning based patient classification using Hepatitis B virus full-length genome quasispecies from Asian and European cohorts
Chronic infection with Hepatitis B virus (HBV) is a major risk factor for the development of advanced liver disease including fibrosis, cirrhosis, and hepatocellular carcinoma (HCC). The relative contribution of virological factors to disease progression has not been fully defined and tools aiding the deconvolution of complex patient virus profiles is an unmet clinical need. Vari