In-the-wild residual data research and privacy

As the world becomes increasingly dependent on technology, researchers endeavor to understand how technology is used, the impact it has on everyday life and the life-cycle and span of digital information. In doing so, researchers are increasingly gathering `real-world' or `in the wild' residual data, obtained from a variety of sources without the explicit consent of the original owners. This data gathering raises significant concerns regarding privacy, ethics and legislation, as well as practical considerations concerning investigator training, data storage, overall security and disposal. This paper surveys recent studies of residual data gathered in the wild and analyses the challenges that were faced. Taking these insights, the paper presents a compendium of practices for addressing the issues that arise in in the wild residual data research. The practices presented in this paper can be used to critique current projects and assess the feasibility of proposed future research

Efficient instruction level simulation of computers

Journal ArticleA technique for creating efficient, yet highly accurate, instruction level simulation models of computers is described. In contrast to traditional approaches that use a software interpreter, this technique employs direct execution of application programs on the host computer. An assembly language program for the machine to be modeled is decompiled to a high level language, instrumented, and then recompiled and executed on the host computer. A prototype implementation modeling the Motorola MC68010 microprocessor is described, and the efficiency and accuracy of this prototype is reported. It is demonstrated that the direct execution technique can be used to produce accurate simulation models which are orders of magnitude faster than traditional, register transfer level simulators

Business strategies and employment decisions: Interviews with New Zealand firms

A firm’s business strategy does not exist in a vacuum; it requires employees to implement it. However, firms often say that they have difficulties hiring certain types of employees. Through interviews with firms and analysis of the 2008 New Zealand Business Operations Survey, this research explored the interaction between strategy and employees’ skills, and differences between high value-add (HVA) and medium value-add (MVA) firms. MVA firms focused more on production methods, technical skills, and margins over costs. HVA firms focused more on the business skills of a few, professional core employees, as well as the marketing aspects of their products.The research was conducted with funding from the Ministry of Economic Development and the Cross- Department Research Pool (CDRP)

Regulation of KCa2.3 andendothelium-dependent hyperpolarization (EDH) in the rat middle cerebral artery: the role of lipoxygenase metabolites and isoprostanes

Background and Purpose. In rat middle cerebral arteries, endothelium-dependent hyperpolarization (EDH) is mediated by activation of calcium-activated potassium(KCa) channels specifically KCa2.3 and KCa3.1. Lipoxygenase (LOX) products function as endothelium-derived hyperpolarizing factors (EDHFs) in rabbit arteries by stimulating KCa2.3. We investigated if LOX products contribute to EDH in rat cerebral arteries. Methods. Arachidonic acid (AA) metabolites produced in middle cerebral arteries were measured using HPLC and LC/MS. Vascular tension and membrane potential responses to SLIGRL were simultaneously recorded using wire myography and intracellular microelectrodes. Results. SLIGRL, an agonist at PAR2 receptors, caused EDH that was inhibited by a combination of KCa2.3 and KCa3.1 blockade. Non-selective LOX-inhibition reduced EDH, whereas inhibition of 12-LOX had no effect. Soluble epoxide hydrolase (sEH) inhibition enhanced the KCa2.3 component of EDH. Following NO synthase (NOS) inhibition, the KCa2.3 component of EDH was absent. Using HPLC, middle cerebral arteries metabolized 14C-AA to 15- and 12-LOX products under control conditions. With NOS inhibition, there was little change in LOX metabolites, but increased F-type isoprostanes. 8-iso-PGF2α inhibited the KCa2.3 component of EDH. Conclusions. LOX metabolites mediate EDH in rat middle cerebral arteries. Inhibition of sEH increases the KCa2.3 component of EDH. Following NOS inhibition,loss of KCa2.3 function is independent of changes in LOX production or sEH inhibition but due to increased isoprostane production and subsequent stimulation of TP receptors. These findings have important implications in diseases associated with loss of NO signaling such as stroke; where inhibition of sEH and/or isoprostane formation may of benefit

Tribute to Frederick W. Whiteside, Jr.

A series of tributes to Frederick W. Whiteside, Jr., a professor at the University of Kentucky College of Law

Distribution and Length Frequency of Invasive Lionfish (Pterois sp.) in the Northern Gulf of Mexico

The purpose of this communication is to document continued spatial expansion of lionfish farther west into the northern Gulf of Mexico. Furthermore, we provide the first length— mass relationships and length frequency information for lionfishes captured within the northern GOM based on data collected as part of a broader on—going study of lionfish life history in this region

Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors.

Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning.EaStCHEM, NHS Lothian, Jamie King Cancer Research FundThis is the final version of the article. It first appeared from the Royal Society of Chemistry via http://dx.doi.org/10.1039/C6AN01032

Structural Characterization of Monohydroxyeicosatetraenoic Acids and Dihydroxy- and Trihydroxyeicosatrienoic Acids by ESI-FTICR

The fragmentation characteristics of monohydroxyeicosatetraenoic acids and dihydroxy- and trihydroxyeicosatrienoic acids were investigated by electrospray ionization Fourier transform ion cyclotron resonance (FTICR) mass spectrometry using sustained off-resonance irradiation collision-induced dissociation (SORI-CID) and infrared multiphoton dissociation (IRMPD). The fragmentation patterns of these compounds were associated with the number and positions of the hydroxyl substituents. The fragmentation is more complicated with increasing number of the hydroxyl groups of the compounds. In general, the major carbon–carbon cleavage of [M − H]− ions occurred at the α-position to the hydroxyl group, and the carbon–carbon cleavage occurred when there was a double-bond at the β-position to the hydroxyl group. SORI-CID and IRMPD produced some common fragmentation patterns; however, each technique provided some unique patterns that are useful for structural identification of these compounds. This study demonstrated the application of FTICR via the identification of regioisomers of trihydroxyeicosatrienoic acids in rabbit aorta samples