Hemocompatibility of stent materials: alterations in electrical parameters of erythrocyte membranes

A Basoli1, C Cametti2, F Ginnari Satriani2, P Mariani3, P Severino31Department of Surgery, "P Stefanini," University of Rome "La Sapienza," Rome, Italy; 2Department of Physics, University of Rome "La Sapienza," Rome Italy; 3Department of Internal Medicine, University of Rome "La Sapienza," Rome, ItalyBackground: It is presently unknown if stents used in the correction of artery stenosis are fully hemocompatible or if their implantation causes alterations at the level of the plasma membrane in red blood cells.Methods: We addressed this important issue by measuring the passive electrical properties of the erythrocyte membrane before and after stent insertion by means of dielectric relaxation spectroscopy in the radiowave frequency range in a series of patients who were undergoing standard surgical treatment of arterial disease.Results: Our findings provide evidence that full hemocompatibility of stents has not yet been reached, and that there are some measurable alterations in the passive electrical behavior of the red blood cell membrane induced by the presence of the stent.Conclusion: It is possible that these changes do not have any physiological significance and simply reflect the intrinsic variability of biological samples. However, caution is urged, and the technique we describe here should be considered when investigating the hemocompatibility of a medical device at a cell membrane level.Keywords: hemocompatibility, stent, arterial disease, cell membran

Coherent States Expectation Values as Semiclassical Trajectories

We study the time evolution of the expectation value of the anharmonic oscillator coordinate in a coherent state as a toy model for understanding the semiclassical solutions in quantum field theory. By using the deformation quantization techniques, we show that the coherent state expectation value can be expanded in powers of $\hbar$ such that the zeroth-order term is a classical solution while the first-order correction is given as a phase-space Laplacian acting on the classical solution. This is then compared to the effective action solution for the one-dimensional \f^4 perturbative quantum field theory. We find an agreement up to the order \l\hbar, where \l is the coupling constant, while at the order \l^2 \hbar there is a disagreement. Hence the coherent state expectation values define an alternative semiclassical dynamics to that of the effective action. The coherent state semiclassical trajectories are exactly computable and they can coincide with the effective action trajectories in the case of two-dimensional integrable field theories.Comment: 20 pages, no figure

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Low-frequency collective vibrational modes in proteins have been proposed as being responsible for efficiently directing biochemical reactions and biological energy transport. However, evidence of the existence of delocalized vibrational modes is scarce and proof of their involvement in biological function absent. Here we apply extremely sensitive femtosecond optical Kerr-effect spectroscopy to study the depolarized Raman spectra of lysozyme and its complex with the inhibitor triacetylchitotriose in solution. Underdamped delocalized vibrational modes in the terahertz frequency domain are identified and shown to blue-shift and strengthen upon inhibitor binding. This demonstrates that the ligand-binding coordinate in proteins is underdamped and not simply solvent-controlled as previously assumed. The presence of such underdamped delocalized modes in proteins may have significant implications for the understanding of the efficiency of ligand binding and protein–molecule interactions, and has wider implications for biochemical reactivity and biological function