True substrates: The exceptional resolution and unexceptional preservation of deep time snapshots on bedding surfaces

Abstract: Rock outcrops of the sedimentary–stratigraphic record often reveal bedding planes that can be considered to be true substrates: preserved surfaces that demonstrably existed at the sediment–water or sediment–air interface at the time of deposition. These surfaces have high value as repositories of palaeoenvironmental information, revealing fossilized snapshots of microscale topography from deep time. Some true substrates are notable for their sedimentary, palaeontological and ichnological signatures that provide windows into key intervals of Earth history, but countless others occur routinely throughout the sedimentary–stratigraphic record. They frequently reveal patterns that are strikingly familiar from modern sedimentary environments, such as ripple marks, animal trackways, raindrop impressions or mudcracks: all phenomena that are apparently ephemeral in modern settings, and which form on recognizably human timescales. This paper sets out to explain why these short‐term, transient, small‐scale features are counter‐intuitively abundant within a 3.8 billion year‐long sedimentary–stratigraphic record that is known to be inherently time‐incomplete. True substrates are fundamentally related to a state of stasis in ancient sedimentation systems, and distinguishable from other types of bedding surfaces that formed from a dominance of states of deposition or erosion. Stasis is shown to play a key role in both their formation and preservation, rendering them faithful and valuable archives of palaeoenvironmental and temporal information. Further, the intersection between the time–length scale of their formative processes and outcrop expressions can be used to explain why they are so frequently encountered in outcrop investigations. Explaining true substrates as inevitable and unexceptional by‐products of the accrual of the sedimentary–stratigraphic record should shift perspectives on what can be understood about Earth history from field studies of the sedimentary–stratigraphic record. They should be recognized as providing high‐definition information about the mundane day to day operation of ancient environments, and critically assuage the argument that the incomplete sedimentary–stratigraphic record is unrepresentative of the geological past

Managing perennial revegetation in a changing climate: some lessons from ecohydrology

Interest in understanding the impacts of land use and climate change on ecosystem processes has emerged as a major area of research spanning the biological and physical sciences. South-West Australia faces a drying climate under all Global Climate Model (GCM) scenarios and over the last three decades there has already been a major decline in the volume of surface water resources available for the metropolitan water supply of the region. Climate change has been superimposed on major land use changes that have altered the water and salt balances of many catchments in this part of Australia. In the drier agricultural regions of South-West Australia that experience an annual water deficit, land clearing has resulted in increased groundwater recharge and there has been ongoing interest in the use of perennial vegetation to control groundwater rise by enhancing transpiration losses. Ecological optimality provides a first-order framework for understanding the relation between climate, leaf area index and biomass, which in turn influences catchment evapotranspiration (ET) and carbon sequestration. We review the results from a number of revegetation studies to understand the factors limiting the growth and survival of woody perennials in the landscape. By examining the inter-annual variations in leaf area index of native forested catchments using data from NASA's MODIS Moderate Resolution Imaging Spectroradiometer, we first relate LAI to climate indices and then develop allometric equations to estimate wood mass and carbon storage. Assuming that native perennial vegetation is in dynamic equilibrium with climate and provides a 'reference' state , we identify conditions under which perennial revegetation schemes can be deliberately designed to move outside the ecologically optimal range to achieve specific land management objectives

TLX1/HOX11 transcription factor inhibits differentiation and promotes a non-haemopoietic phenotype in murine bone marrow cells

The TLX/HOX11 subfamily of divergent homeobox genes are involved in various aspects of embryogenesis and, in the case of TLX1/HOX11 and TLX3/HOX11L2, feature prominently as oncogenes in human T-cell acute lymphoblastic leukaemia. TLX1 possesses immortalising activity in a wide variety of blood cell lineages, however, the effect of this oncogene on haemopoietic cell differentiation has not been fully investigated. We therefore constitutively expressed TLX1 in murine bone marrow or fetal liver cells using retroviral transfer followed by transplantation and/or in vitro culture. TLX1 was found to dramatically alter haemopoiesis, promoting the emergence of a non-haemopoietic CD45- CD31+ cell population while markedly inhibiting erythroid and granulocytic cell differentiation. To identify genetic programs perturbed by TLX1, a comparison of transcript profiles from J2E erythroid cells with and without enforced TLX1 expression was undertaken. This revealed a pattern of gene expression indicative of enhanced proliferation coupled to differentiation arrest. Of the genes identified, two, KIT and VEGFC, were found to be potential TLX1 targets based on transcriptional assays. These results demonstrate that TLX1 can act broadly to impair haemopoiesis and divert differentiation to an alternative fate. This may account for its ability to promote the pre-leukaemic state via perturbation of specific gene expression programs