217 research outputs found

    Financing Health Care for the Aged

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    Characterization and expression analysis of the Bartonella bacilliformis groES-EL operon

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    Applying Multiple Intelligences Theory to the Basic Public Speaking Course

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    This article examines the theory of Multiple Intelligences (MI) (Gardner, 1983; 1993) as it applies to the basic public speaking course. According to MI theory, intelligence is not a single dimension but is a composite of several aptitudes and talents. Gardner believes that individuals possess more than one intelligence, and MI theory defines seven. We argue that the basic public speaking course is an excellent forum for addressing students’ multiple intelligences while teaching oral and written communication skills. This paper introduces MI theory and provides suggested course assignments and activities that correspond with the multiple intelligences

    Characterization and expression analysis of the groESL operon of Bartonella bacilliformis

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    The groESL operon of Bartonella bacilliformis, a facultative intracellular, Gram-negative bacterium and etiologic agent of Oroya Fever, was characterized. Sequence analysis revealed an operon containing two genes of 294 (groES) and 1632 nucleotides (groEL) separated by a 55-nt intergenic spacer. The operon is preceded by a 72-nt ORF (ORF1) that encodes a hypothetical protein with homology to a portion of the HrcA repressor for groESL. A divergent fumarate hydratase C (fumC) gene lies further upstream. Deduced amino acid sequences for B. bacilliformis GroEL and GroES revealed a high degree of identity with homologues from other Bartonella and alpha-Protebacteria. A single transcriptional start site (TSS) was mapped 79 nucleotides upstream of the groES start codon, regardless of incubation temperature. The TSS was located immediately 5\u27 to a potential controlling inverted repeat of chaperonin expression (CIRCE) element and is preceded by a sigma70-like promoter. The operon is followed by a predicted rho-independent transcriptional terminator. Northern blot analysis indicated that groES and groEL are co-transcribed as a single mRNA of approximately 2.4 kb. A 6-h time course analysis by qRT-PCR showed that groEL expression increases 1.3-fold within 30 min of a temperature upshift from 30 to 37 degrees C, with maximum transcription reached after 60 min (approximately 4.3-fold), followed by a steady decrease to background (30 degrees C) transcription levels by 6 h. Western blot analysis revealed a 1.4- and 1.5-fold increase in GroEL synthesis following a temperature upshift or by inhibiting DNA supercoiling with coumermycin A1, respectively. Functional expression and complementation of temperature-sensitive Escherichia coli groES or groEL mutants with the cloned operon allowed them to grow at otherwise restrictive temperatures

    Two quantum Ising algorithms for the shortest-vector problem

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    Quantum computers are expected to break today's public key cryptography within a few decades. New cryptosystems are being designed and standardized for the postquantum era, and a significant proportion of these rely on the hardness of problems like the shortest-vector problem to a quantum adversary. In this paper we describe two variants of a quantum Ising algorithm to solve this problem. One variant is spatially efficient, requiring only O ( N log 2 N ) qubits, where N is the lattice dimension, while the other variant is more robust to noise. Analysis of the algorithms' performance on a quantum annealer and in numerical simulations shows that the more qubit-efficient variant will outperform in the long run, while the other variant is more suitable for near-term implementation

    An introduction to crowdsourcing for language and multimedia technology research

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    Language and multimedia technology research often relies on large manually constructed datasets for training or evaluation of algorithms and systems. Constructing these datasets is often expensive with significant challenges in terms of recruitment of personnel to carry out the work. Crowdsourcing methods using scalable pools of workers available on-demand offers a flexible means of rapid low-cost construction of many of these datasets to support existing research requirements and potentially promote new research initiatives that would otherwise not be possible

    Effects of ethical leadership on emotional exhaustion in high moral intensity situations

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    © 2015 Elsevier Inc. Emotional exhaustion is a threat to standard operations, particularly in organizations in which physical safety is at risk. High moral intensity is inherent in such organizations due to the magnitude of consequences associated with ethical/unethical conduct. The authors proposed a psychological process in which ethical leadership affects emotional exhaustion directly and indirectly through team cohesion. As military operational contexts typically are (or frequently have the potential to become) high moral intensity situations, the authors tested their model among 338 military personnel deployed in combat zones. They found that: (1) team cohesion partially mediated the relationship between ethical leadership and emotional exhaustion, and (2) this psychological process of direct and indirect effects of ethical leadership did not hold among individuals approaching the low end of conscientiousness

    Rapid Synthesis of [Au₂₅(Cys)₁₈] Nanoclusters via Carbon Monoxide in Microfluidic Liquid-Liquid Segmented Flow System and their Antimicrobial Performance

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    Atomically precise thiolate-gold nanoclusters with well-defined structures attract attention for use in various applications. However, most of the recently reported synthetic methods rely on prolonged synthesis times (a few hours to days) in order to produce high purity materials with a single cluster size. Such extended synthesis times make these processes ill-suited for adaptation to industrial scale production with continuous flow. In this work, an improved method for the synthesis of thiolated Au25 nanoclusters is presented utilising a microfluidic system and CO-mediated reduction. The optimized system, based on a coiled flow inverter with inner diameter of 1 mm operating at 80 °C and 500 kPa took only 3 min for the synthesis of atomically precise cysteine-capped [Au25(Cys)18] nanoclusters, as characterized by ultraviolet–visible spectroscopy and electrospray ionization mass spectrometry. The productivity of the system was increased by using higher reactant concentrations which led to a throughput of 0.9 gAu per day, without changing the reaction time or affecting the product purity. The Au nanoclusters were used as photobactericidal enhancement materials. In antimicrobial testing against S. aureus, encapsulation of the Au nanoclusters into crystal violet impregnated silicone showed high photobactericidal activity (~1.7 log reduction in viable bacteria) upon 6 h illumination of white light at ~312 lx, while crystal violet did not show significant photobactericidal activity on its own
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