Mammalian Cytotoxicity Study of Novel Organometallic Complexes

Antibiotic resistance is becoming more and more prevalent in our society to the point that even simple diseases can be life threatening. Thus, there is a significant need for novel antibiotics that can combat these diseases and reduce future complications when a disease becomes resistant to a treatment. While there is a need for more novel antibiotics, one must also understand the nature of the compound and all of the effects that it will have on both the pathogen and the host body before it is distributed. The compounds that we are studying are novel organometallics. While there is minimal research on these compounds, there is evidence that there may be cytotoxic effects against mammalian cells. The purpose of this study was to determine the cytotoxic effects of the organometallic compounds against 2 different human cell lines using a Resazurin Cell Viability Assay. The data was analyzed using GraphPad Prism software to calculate IC50 values . In the absence of the metal ions, the organic monomer toxicity was cell line dependent, with the A549 cells much more sensitive than the K562 cells. Once metal complexes were formed, the cytotoxicy was generally increased for both cell lines, with IC50 values in the low micromolar range

Development of Novel Organometallic Compounds as Antibiotics

Antimicrobial resistance is an emerging healthcare problem. More than 2.8 million cases of antibiotic-resistant infections are reported each year in the U.S., and the emergence of new antibiotic-resistant microbes is a growing concern. Most scientific research is focused on the exploration and development of common antibiotics, but the search for new drug compounds is a necessity. Organometallic compounds are a class of drugs that have largely been overlooked in antibiotic research. These compounds have unique structures that have an impact on microbial growth. In this study, a series of organometallic compounds were first studied using Kirby-Bauer disk diffusion assays. The compounds were further analyzed using microdilution assays. The half maximal inhibitory concentration (IC50) and Minimum Inhibitory Concentration (MIC) were determined for all organometallic compounds. The results of this study showed that this series of organometallic compounds are effective against Gram-positive bacteria and organometallic compounds with silver ion complexes showed greater inhibition and broader susceptibility profiles overall

Evaluation of Azothioformamides and Their Copper(I) and Silver(I) Complexes for Biological Activity

Redox-active azothioformamides (ATFs) contain an NNCS 1,3-heterodiene motif typically found in other molecular subclasses that exhibit a wide range of cytotoxic and anti-neoplastic effects, either alone or as chelation complexes with various metals. For this study, a small library of ATF compounds was synthesized and tested across a range of microbes, fungi, and cancer cell lines for biological activity, both alone and as metal chelates of copper(I) and silver(I) salts. Alone, the ATF compounds exhibited little antimicrobial activity, but all inhibited the cell growth of A549 lung carcinoma cells (IC50 values of 1–6 μM). As copper(I) and silver(I) coordination complexes, several of the ATFs showed antimicrobial activity against gram positive Staphylococcus aureus and Bacillus subtilis cells (IC50 ∼ 5–20 μM) and the fungi Candida albicans (IC50 ∼ 8–12 μM); as well as cytotoxicity against both lung carcinoma A549 cells and lymphoblastic leukemia K562 cells