EVALUATING THE ANTIPROLIFERATIVE POTENTIAL OF METHONOLIC LEAF EXTRACT OF CASSIA NIGRICANS

Objective: It is well established that plants have always been useful source as anticancer compounds. This study was attempted to investigate the in vitro anti-cancer potential of methonolic extract of Cassia nigricans on breast cancer MCF-7 cell lines.Methods: The methanol extract of C. nigricans was screened for its anti-proliferative effect against MCF-7 (Breast cancer) cell lines using MCF-7 cells seeded 96 well plates.Results: Extract was exposed with MCF-7 cell lines for 24h and 72h at a range of increasing concentrations (0-500μg/ml) in order to obtain a dose-response graph and IC50 value. The C. nigricans extract showed cytotoxic effect in MCF-7cells with IC50 of 82.6μg/ml.Conclusion: The C. nigricans extract showed effective cytotoxic activity in a dose and time dependent manner. Future work will be interesting to know the chemical composition and also better understand the mechanism of action present in the extract for developing it as drug for therapeutic application.Â

COMPARATIVE PHYTOCHEMICAL PROFILE AND ANTIOXIDANT PROPERTY OF BARK, FLOWERS AND LEAVES EXTRACTS OF SIMAROUBA GLAUCA

Objective: The current study was to evaluate and compare the phytochemical constituents and antioxidant activity of bark, flowers and leaves of the tree Simarouba glauca. Methods: The solvent extraction of phytochemicals was carried out using Soxhlet apparatus with ethanol, chloroform, methanol, and water. The antioxidant property was determined by 2,2-Diphenyl-1-picrylhydrazyl, hydrogen peroxide free radical scavenging, reducing power assay, and nitric oxide radical scavenging assay using gallic acid and ascorbic acid as the standards. Results: The extraction yield was found maximum in the water extract of flower (3.7% w/w). Qualitative and quantitative analysis of phytoconstituents showed that the highest amount of alkaloids and flavonoid content (2.1% w/w) and (3.9% w/w), respectively, was in the chloroform extract of the flower. Phenol and carbohydrate constituent was found to be highest in the methanol extract of leaves 2.5% w/w and 2.2% w/w, respectively. The antioxidant assays showed that the bark possessed maximum antioxidant activity. The water extracts of S. glauca bark exhibited scavenging property (90%) with an IC50 value 39.63 μg/ml, and the least activity (56%) was observed in the methanol extracts of leaves with an IC50 value of 62.96 μg/ml of S. glauca. Conclusion: The study concluded that the water extract of the bark is a potent antioxidant compared to leaves and flowers. Further, in vivo studies are essential to enumerate its medicinal use and prove its efficacy in therapeutic applications