35 research outputs found
Complexation of Amino Compounds by 18C6 Improves Selectivity by IMS-IMS-MS: Application to Petroleum Characterization
No full textDifferentiation of Compact and Extended Conformations of Di-Ubiquitin Conjugates with Lysine-Specific Isopeptide Linkages by Ion Mobility-Mass Spectrometry
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An Ion Mobility/Ion Trap/Photodissociation Instrument for Characterization of Ion Structure
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Prediction of peptide drift time in ion mobility mass spectrometry from sequence-based features
No full textArtificial neural networks for the prediction of peptide drift time in ion mobility mass spectrometry
No full textIon mobility-mass spectrometry analysis of large protein complexes.
No full textHere we describe a detailed protocol for both data collection and interpretation with respect to ion mobility-mass spectrometry analysis of large protein assemblies. Ion mobility is a technique that can separate gaseous ions based on their size and shape. Specifically, within this protocol, we cover general approaches to data interpretation, methods of predicting whether specific model structures for a given protein assembly can be separated by ion mobility, and generalized strategies for data normalization and modeling. The protocol also covers basic instrument settings and best practices for both observation and detection of large noncovalent protein complexes by ion mobility-mass spectrometry
