20 research outputs found
A simulation-based curriculum to introduce key teamwork principles to entering medical students
Serial blood cytokine and chemokine mRNA and microRNA over 48 h are insult specific in a piglet model of inflammation-sensitized hypoxia-ischaemia.
BACKGROUND: Exposure to inflammation exacerbates injury in neonatal encephalopathy (NE). We hypothesized that brain biomarker mRNA, cytokine mRNA and microRNA differentiate inflammation (E. coli LPS), hypoxia (Hypoxia), and inflammation-sensitized hypoxia (LPS+Hypoxia) in an NE piglet model. METHODS: Sixteen piglets were randomized: (i) LPS 2 μg/kg bolus; 1 μg/kg infusion (LPS; n = 5), (ii) Saline with hypoxia (Hypoxia; n = 6), (iii) LPS commencing 4 h pre-hypoxia (LPS+Hypoxia; n = 5). Total RNA was acquired at baseline, 4 h after LPS and 1, 3, 6, 12, 24, 48 h post-insult (animals euthanized at 48 h). Quantitative PCR was performed for cytokines (IL1A, IL6, CXCL8, IL10, TNFA) and brain biomarkers (ENO2, UCHL1, S100B, GFAP, CRP, BDNF, MAPT). MicroRNA was detected using GeneChip (Affymetrix) microarrays. Fold changes from baseline were compared between groups and correlated with cell death (TUNEL) at 48 h. RESULTS: Within 6 h post-insult, we observed increased IL1A, CXCL8, CCL2 and ENO2 mRNA in LPS+Hypoxia and LPS compared to Hypoxia. IL10 mRNA differentiated all groups. Four microRNAs differentiated LPS+Hypoxia and Hypoxia: hsa-miR-23a, 27a, 31-5p, 193-5p. Cell death correlated with TNFA (R = 0.69; p < 0.01) at 1-3 h and ENO2 (R = -0.69; p = 0.01) at 48 h. CONCLUSIONS: mRNA and miRNA differentiated hypoxia from inflammation-sensitized hypoxia within 6 h in a piglet model. This information may inform human studies to enable triage for tailored neuroprotection in NE. IMPACT: Early stratification of infants with neonatal encephalopathy is key to providing tailored neuroprotection. IL1A, CXCL8, IL10, CCL2 and NSE mRNA are promising biomarkers of inflammation-sensitized hypoxia. IL10 mRNA levels differentiated all three pathological states; fold changes from baseline was the highest in LPS+Hypoxia animals, followed by LPS and Hypoxia at 6 h. miR-23, -27, -31-5p and -193-5p were significantly upregulated within 6 h of a hypoxia insult. Functional analysis highlighted the diverse roles of miRNA in cellular processes
A pilot cohort study of cerebral autoregulation and 2-year neurodevelopmental outcomes in neonates with hypoxic-ischemic encephalopathy who received therapeutic hypothermia
Are midwifery students adequately prepared for vaginal breech birth in clinical practice?
The preparedness level of final year medical students for an adequate medical approach to emergency cases: computer-based medical education in emergency medicine
Misoprostol vaginal insert versus misoprostol vaginal tablets for the induction of labour: a cohort study
Effect of Sample Processing Delays on the Values of Serum Based Biomarkers of Brain Injury Collected from the Umbilical Cord Blood of Neonates
Predictors of response after a second attempt of pharmacological labor induction: a retrospective study
Serial blood cytokine and chemokine mRNA and microRNA over 48 h are insult specific in a piglet model of inflammation-sensitized hypoxia–ischaemia
Collecting data on TMTs’ organizational design: good practices from the StiMa project
Studying how Top Management Teams (TMTs) organize their managerial labor and decision-making processes is a relevant research avenue in light of the prominence of TMTs within modern firms. However, data on TMTs’ organizational design are usually not available from secondary sources and must instead be collected through surveys given to top executives. To help researchers in this difficult endeavor, the present paper offers a set of good practices for designing and implementing surveys on TMTs’ organizational design. To this end, we use as a basis the first-hand experience that we gained by working on the StiMa project, a large-scale data collection effort concerning the TMTs of a representative sample of Italian firms. We describe in detail all of the steps that we followed in preparing and administering the survey and in setting up and executing the additional data collection from secondary sources, which integrates survey data. We are confident that the lessons we learned from the StiMa project will help scholars in gathering better data on TMTs’ organizational design, thus advancing academics’ and practitioners’ conversations on the topic