A EMBRAPA no Pantanal Mato-Grossense.

A Unidade de Execucao de Pesquisa de Ambito Estadual de Corumba, da Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), tem como objetivos avaliar as potencialidades dos diferentes ecossistemas da regiao e estabelecer o suporte cientifico que devera nortear sua exploracao em bases economicas e ecologicas. Espera-se que, com a geracao e adaptacao de tecnologia, seja possivel, a curto e medio prazo, obter incrementos positivos nos indices de produtividade da pecuaria regional. Pesquisas em andamento estao mostrando esta tendencia, o que evidencia o potencial da regiao a novas tecnologias.bitstream/item/37788/1/DOC01.pd

Stability and microstructure of powdered pulp of the Palmer mango obtained by the process of lyophilisation.

ABSTRACT The aim of this work was to study the stability of powdered mango pulp obtained through the process of lyophilisation, as well as its structural characteristics using electron microscopy analysis. In response to an earlier experimental design using maltodextrin concentrations of 0%, 10%, 20% and 30% and periods of 24 and 30 hours, the condition of 20% maltodextrin and 24 hours drying was chosen as the best for drying mango in a freeze dryer. The stability study was therefore carried out using powdered mango pulp obtained under the above best-drying conditions, stored for 90 days and monitored every two weeks by analysis of the moisture, ascorbic acid content, b* colorimetric parameter and hygroscopicity. Storage was at room temperature (27 °C) in vacuum and non-vacuum laminated packaging, and in non-vacuum plastic packaging. The vacuum laminated packaging maintained the initial characteristics of the stored product for longer, especially the ascorbic acid content, hygroscopicity and b* colour parameter. The microstructure of the sample was then studied under different concentrations of maltodextrin (0%, 10%, 20% and 30%) so as to understand the effect of the drying agent on the final product. Close-up images of the powder revealed the porous surface formation and the effect of the agent on particle size, where higher concentrations of maltodextrin resulted in more porous powders with smaller particles.</div

Immunoexpression Of α2-integrin And Hsp47 In Hereditary Gingival Fibromatosis And Gingival Fibromatosis-associated Dental Abnormalities

Objective: The purpose of the present study was to investigate the expression of the α2-integrin subunit and heat shock protein 47 (Hsp47) in two families with isolated gingival fibromatosis (GF) form and one family with GF associated with dental abnormalities and normal gingiva (NG). Study Design: Immunohistochemistry was performed with antibodies against α2-integrin and Hsp47 in specimens from two unrelated families with hereditary gingival fibromatosis (Families 1 and 2) and from one family with a gingival fibromatosis-associated dental abnormality (Family 3); NG samples were used for comparison. The results were analysed statistically. Results: Immunoreactivity for α2-integrin and Hsp47 was observed in the nucleus of epithelial cells of both the basal and suprabasal layer and a more discreet signal was noted in connective tissue in all study samples. Hsp47 showed higher immunoreactivity in Family 2 compared with the other families (p≤0.05). Despite the markup α2-integrin was higher in Family 3 there was no statistically significant difference between the families studied (p≥0.05). Conclusions: Our results confirmed the heterogeneity of GF, such that similar patterns of expression of the condition may show differences in the expression of proteins such as Hsp47. Although no difference in α2-integrin expression was observed between GF and NG groups, future studies are necessary to determine the exact role of this protein in the various forms of GF and whether it contributes to GF pathogenesis. © Medicina Oral S. L. C.I.F. B 96689336 - pISSN 1698-4447 - eISSN: 1698-6946.181e45e48Takagi, M., Yamamoto, H., Mega, H., Hsieh, K.J., Shioda, S., Enomoto, S., Heterogeneity in the gingival fibromatoses (1991) Cancer, 68, pp. 2202-2212Dongari-Bagtzoglou, A., Drug-associated gingival enlargement (2004) J Periodontol, 75, pp. 1424-1431Coletta, R.D., Graner, E., Hereditary gingival fibromatosis: A systematic review (2006) J Periodontol, 77, pp. 753-764Hakkinen, L., Csiszar, A., Hereditary gingival fibromatosis: Characteristics and novel putative pathogenic mechanisms (2007) J Dent Res, 86, pp. 25-34Singer, S.L., Goldblatt, J., Hallan, L.A., Winters, J.C., Hereditary gingival fibromatosis with a recessive mode of inheritance. Case reports (1993) Aust Dent J, 38, pp. 427-432Martelli-Júnior, H., Bonan, P.R., Dos Santos, L.A., Santos, S.M., Cavalcanti, M.G., Coletta, R.D., Case reports of a new syndrome associating gingival fibromatosis and dental abnormalities in a consanguineous family (2008) J Periodontol, 79, pp. 1287-1296Ivarsson, M., McWhirter, A., Black, C.M., Rubin, K., Impaired regulation of collagen pro-α1(I) mRNA and change in pattern of collagen-binding integrins on scleroderma fibroblasts (1993) J Invest Dermatol, 101, pp. 216-221Langholz, O., Rockel, D., Mauch, C., Kozlowska, E., Bank, I., Krieg, T., Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by α1β1 and α2β1 integrins (1995) J Cell Biol, 131, pp. 1903-1915Riikonen, T., Westermarck, J., Koivisto, L., Broberg, A., Kahari, V.M., Heino, J., Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression (1995) J Biol Chem, 270, pp. 13548-13552Fujimura, T., Moriwaki, S., Imokawa, G., Takema, Y., Crucial role of fibroblasts integrins alpha2 and beta1 in maintaining the structural and mechanical properties of the skin (2007) J Dermatol Sci, 45, pp. 45-53Nagata, K., Expression and function of heat shock protein 47: A collagen-specific molecular chaperone in the endoplasmic reticulum (1998) Matrix Biol, 16, pp. 379-386Bozzo, L., Almeida, O.P., Scully, C., Aldred, M.J., Hereditary gingival fibromatosis. Report of an extensive four-generation pedigree (1994) Oral Surg Oral Med Oral Pathol, 78, pp. 452-454Martelli-Júnior, H., Lemos, D.P., Silva, C.O., Graner, E., Coletta, R.D., Hereditary gingival fibromatosis: Report of a five-generation family using cellular proliferation analysis (2005) J Periodontol, 76, pp. 2299-2305Vigneswaran, N., Zhao, W., Dassanayake, A., Muller, S., Miller, D.M., Zacharias, W., Variable expression of cathepsin B and D correlates with highly invasive and metastatic phenotype of oral cancer (2000) Hum Pathol, 31, pp. 931-937Zhou, J., Meng, L.Y., Ye, X.Q., von der Hoff, J.W., Bian, Z., Increased expression of integrin alpha 2 and abnormal response to TGF-β1 in hereditary gingival fibromatosis (2009) Oral Dis, 15, pp. 414-421Nagata, K., Hosokawa, N., Regulation and function of collagen-specific molecular chaperone, HSP47 (1996) Cell Struct Funct, 21, pp. 425-430Bolcato-Bellemin, A.-L., Elkaim, R., Tenenbaum, H., Expression of RNAs encoding for α and β integrin subunits in periodontitis and in cyclosporin A gingival overgrowth (2003) J Clin Periodontol, 30, pp. 937-943Kataoka, M., Seto, H., Wada, C., Kido, J., Nagata, T., Decreased expression of α2 integrin in fibroblasts isolated from cyclosporin A-induced gingival overgrowth in rats (2003) J Periodontal Res, 38, pp. 533-537Slambrouk, S.V., Jenkins, A.R., Romero, A.E., Steelant, W.F.A., Reorganization of the integrin α2 subunit controls cell adhesion and cancer cell invasion in prostate cancer (2009) Int J Oncol, 34, pp. 1717-1726O'Sullivan, J., Bitu, C.C., Daly, S.B., Urquhart, J.E., Barron, M.J., Bhaskar, S.S., Whole-exome sequencing identifies FAM20A mutations as a cause of amelogenesis imperfect and gingival hyperplasia syndrome (2011) Am J Hum Genet, 88, pp. 616-620Martelli-Júnior, H., Santos, C.O., Bonan, P.R., Moura, P.F., Bitu, C.C., León, J.E., Minichromosome maintenance 2 and 5 expression is increased in the epithelium of hereditary gingival fibromatosis associated with dental abnormalities (2011) Clinics, 66, pp. 753-757Shiuan-Shinn, L., Ling-Hsien, T., Yi-Ching, L., Chung-Hung, T., Yu-Chao, C., Heat shock protein 47 in oral squamous cell carcinomas and upregulated by arecoline in human oral ephitelial cells (2011) J Oral Pathol Med, 40, pp. 390-396Tagushi, T., Nazneen, A., Al-Shihri, A.A., Turkistani, K.A., Razzaque, M.S., Heat shock protein 47: A novel biomarker of phenotypically altered collagen-producing cells (2011) Acta Histochem Cytochem, 44, pp. 35-41Totan, S., Echo, A., Yuksel, E., Heat shock proteins modulate keloid formation (2011) Eplasty, 11, pp. 190-20

Numerical Study and Geometric Investigation of the Influence of Rectangular Baffles over the Mixture of Turbulent Flows into Stirred Tanks

The present work aims to define strategies for numerical simulation of the mixture of turbulent flows in a stirred tank with a low computational effort, and to investigate the influence of the geometry of four rectangular baffles on the problem of performance. Two computational models based on momentum source and sliding mesh are validated by comparison with experimental results from the literature. For both models, the time‐averaged conservation equations of mass, momentum and transport of the mixture are solved using the finite volume method (FVM) (FLUENT® v.14.5). The standard k–ε model is used for closure of turbulence. Concerning the geometrical investigation, constructal design is employed to define the search space, degrees of freedom and performance indicators of the problem. More precisely, seven configurations with different width/length (L/B) ratios for the rectangular baffles are studied and compared with an unbaffled case. The momentum source model leads to valid results and significantly reduces the computational effort in comparison with the sliding mesh model. Concerning the design, the results indicate that the case without baffles creates the highest magnitude of turbulence kinetic energy, but poorly distributes it along the domain. The best configuration, (L/B)o = 1.0, leads to a mixture performance nearly two times superior than the case without baffles

Micronutrientes e óxidos de ferro em coprólitos de minhocas produzidos em um Latossolo Vermelho distroférrico (Oxisol) sob diferentes sistemas de manejo.

As estruturas biogênicas produzidas pelas minhocas modificam os atributos químicos e mineralógicos do solo. Estas alterações são pouco conhecidas em solos altamente intemperizados neotropicais invadidos por minhocas peregrinas e exóticas. Este trabalho teve como objetivo avaliar as alterações na disponibilidade de micronutrientes e nos óxidos de ferro de um Latossolo Vermelho distroférrico (LVdf) (Oxisol) sob diferentes condições de manejo quando invadidos pelas minhocas Pontoscolex corethrurus e Amynthas gracilis. Os micronutrientes analisados foram zinco (Zn), cobre (Cu), manganês (Mn) e ferro (Fe). Os óxidos de ferro foram analisados através de métodos de dissolução seletiva quantificando-se as alterações nos teores de ferro livre (Fed) e amorfo (Feo) e alumínio e manganês presentes nos óxidos de ferro por substituição isomórfica livres (Ald e Mnd) e amorfos (Alo e Mno). Solo foi coletado sob mata nativa, dois agroecossistemas de plantio direto e uma pastagem, seco ao ar, peneirado e umedecido para a incubação das minhocas e posterior obtenção de coprólitos. A análise dos atributos químicos e mineralógicos dos coprólitos mostrou que, de modo geral, foram observados aumentos nos teores de Fed e Feo nos coprólitos comparados ao solo controle. O oposto foi observado para os teores de Ald e Alo, havendo diminuição dos teores nos coprólitos. Para os teores de Mnd e Mno não houve diferenças significativas. Os coprólitos concentraram maiores teores de Zn, Cu e Mn, mas tiveram teores mais baixos de Fe disponível. A atividade das minhocas influiu, em geral, de forma positiva sobre os teores de micronutrientes e de óxidos de ferro livre e amorfo no LVdf sob diferentes sistemas de manejo estudado

Caracterização do rebanho bovino no município de Mucajaí no Estado de Roraima.

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In vitro susceptibility of Corynebacterium pseudotuberculosis from goats to antimicrobials.

Corynebacterium pseudotuberculosis is a pleomorphic gram-positive bacterium that causes caseous lymphadenitis in goats and sheep

Analysis of Stationary- and Synchronous-Reference Frames for Three-Phase Three-Wire Grid-Connected Converter AC Current Regulators

The current state of the art shows that unbalance and distortion on the voltage waveforms at the terminals of a grid-connected inverter disturb its output currents. This paper compares AC linear current regulators for three-phase three-wire voltage source converters with three different reference frames, namely: (1) natural (abc), (2) orthogonal stationary (αβ), and (3) orthogonal synchronous (dq). The quantitative comparison analysis is based on mathematical models of grid disturbances using the impedance-based analysis, the computational effort assessment, as well as the steady-state and transient performance evaluation based on experimental results. The control scheme devised in the dq-frame has the highest computational effort and inferior performance under negative-sequence voltage disturbances, whereas it shows superior performance under positive-sequence voltages among the reference frames evaluated. In contrast, the stationary natural frame abc has the lowest computational effort due to its straightforward implementation, with similar results in terms of steady-state and transient behavior. The αβ-frame is an intermediate solution in terms of computational cost.This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).fi=vertaisarvioitu|en=peerReviewed