Rutin-functionalized multi-walled carbon nanotubes: molecular docking, physicochemistry and cytotoxicity in fibroblasts

Multi-Walled Carbon Nanotubes (MWCNT) have been functionalized with rutin through three steps (i. reaction step; ii. purification step; iii. drying step) and their physicochemical properties investigated with respect to morphological structure, thermal analysis, Fourier Transform Infrared Spectroscopy (FTIR), and cytotoxicity. The molecular docking suggested the rutin-functionalized MWCNT occurred by hydrogen bonds, which was confirmed by FTIR assays, corroborating the results obtained by thermal analyses. A tubular shape, arranged in a three-dimensional structure, could be observed. Mild cytotoxicity observed in 3T3 fibroblasts suggested a doseeffect relationship after exposure. These findings suggest the formation of aggregates of filamentous structures on the cells favoring the cell penetration.The authors acknowledge Classius Ferreira da Silva, from the Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, for the scanning electron microscopy analyses.info:eu-repo/semantics/publishedVersio

Effect of Monocerin, a fungal secondary metabolite, on endothelial cells.

Abstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine

Parâmetros estruturais e produtivos de gramíneas forrageiras tropicais em Caatinga raleada em savana.

Resumo: O raleamento da Caatinga é uma técnica de manipulação que aumenta a luminosidade no estrato herbáceo favorecendo a produção de biomassa. O enriquecimento desse estrato com espécies perenes favorece a redução na estacionalidade de produção e aumenta a biomassa total disponível. Objetivou-se avaliar parâmetros estruturais das espécies Cenchrus ciliares (buffel) e Panicum maximum cv. Massai em um subbosque de caatinga raleada.Edición de las Memorias de la 25a. Reunión de la Asociación Latinoamericana de Producción Animal (ALPA), 2016, Recife, Brasil

Temperatura e umidade relativa nos processos de infecção do agente causal do oídio do cajueiro.

Objetivou-se avaliar os elementos climáticos temperatura e umidade relativa sobre os processos de infecção de Erysiphe quercicola, agente causal do oídio do cajueiro. Foi avaliado in vitro o efeito de onze temperaturas e cinco umidades relativas sobre a germinação, o comprimento do tubo germinativo e a formação de apressórios de conídios do fungo coletados em flores, e o efeito de três temperaturas e quatro umidades relativas sobre os conídios coletados em maturis.bitstream/item/214290/1/BP-203.pd