Potencial climático da região Norte de Minas Gerais para a produção de uvas destinadas à elaboração de vinhos finos.

bitstream/item/55302/1/cot056.pd

Levantamento, catalogação e zoneamento de pragas associadas a essências florestais plantadas no Brasil.

EVINCI. Resumo

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Ploidy levels of short-term (1 and 2 years) and long-term (7 and 10 years) embryogenic cultures as well as of regenerated plantlets of tamarillo were analyzed by flow cytometry and chromosome counts. Embryogenic cultures were induced from expanding leaves cultured in the presence of Picloram or 2,4-dichlorophenoxyacetic acid (2,4-D) and monthly subcultured on the same media. Embryo development and plantlets were obtained following subculture of the embryogenic tissue in auxin free medium containing gibberellic acid (GA3). Seedlings and rooted shoots from axillary shoot proliferation were used as controls. The results showed that in long-term embryogenic cultures the ability to develop somatic embryos and plantlets was reduced. Embryogenic tissues maintained for 10 years were mostly aneuploids of the tetraploid (2n = 4x = 48) level whereas those kept in culture for 7 years or less were also mostly aneuploids but of the diploid (2n = 2x = 24) level. The results obtained by flow cytometry were, in general, consistent with those obtained by chromosome counts. The chromosome alteration observed in the embryogenic tissues was already present after 1 year of culture and increased with culture age, hence impairing the maintenance of these tissues for long periods without affecting chromosome stability of the regenerated plantlets. However, the occurrence of triploids and tetraploids as well as aneuploids can be useful for breeding purposes. A value around 23 pg/2C was found for the genome size of tamarillo largely exceeding the value previously published (15.50 pg/2C).This work was supported by the Portuguese Foundation for Science and Technology (FCT)

Air quality inside a school building: air exchange monitoring, evolution of carbon dioxide and assessment of ventilation strategies

This paper presents an assessment of indoor air quality and various ventilation strategies inside a school building located in the south of Portugal. In the first phase, ventilation rate was experimentally evaluated using the tracer gas method. In the second part, different airflow typologies were investigated and, after calculating the air exchange and flow rates for each of them, the evolution of metabolic carbon dioxide inside the spaces was numerically estimated. Ventilation measurements were made in classrooms, auditorium, offices, staff and computer rooms. The assessment of ventilation was based on evaluating the carbon dioxide produced by the occupants for three ventilation approaches; these were: one based on cross-flow natural ventilation (in current use) and two based on forced ventilation systems. In the case of the forced systems, one was based on providing a constant flow to meet the required Portuguese ventilation standard in the main occupied rooms while the other was an adjusted constant rate based on a simple calculation procedure that took into consideration the air quality needs of all the spaces including corridors and atria. This approach was developed to produce an efficient yet inexpensive ventilation approach that did not incorporate expensive sensors and control systems. Carbon dioxide evolution predictions were made using software that evaluated the thermal response and the air quality of a building with complex topology. The numerical model used to evaluate air quality, was based on mass conservation integral equations in which the final equations system was solved through the Runge-Kutta-Fehlberg method with error control. A statistical study of the occupation cycle in the school building during the day was developed

Micronucleus test and comet assay in erythrocytes of the Amazonian electric fish Apteronotus bonapartii exposed to benzene

In this study we address the genotoxicity and putative mutagenic effects of benzene (BZN) in the erythrocytes of the electric fish Apteronotus bonapartii (Gymnotiformes, Apteronotidae) using the micronucleus test (MN) and comet assay, under controlled laboratory conditions. Electric fish were collected in the Solimões River, Manaus-AM, Brazil, and the specimens were exposed to 10 and 25 ppm concentrations of BZN, in 150L tanks. Blood samples were collected at 0 (T0), 24, 48, 72 and 96 hours of exposure. For the concentration of 10 ppm BZN, the number of comets was significantly higher than T0 levels after exposure of 48 hours, whereas the nuclear abnormalities (including MN) did not show any increase in relation to the controls (T0) up to 96 hours. For the 25 ppm BZN, MN rates presented a significant increase after 72 hours, whereas other types of nuclear abnormalities increased in frequency after various exposure times, ranging from 24 to 72 hours. The number of comets increased significantly from 24 hours onwards for 25 ppm BZN. Both assays also showed a gradual increase in the number of damaged cells after longer exposure periods, indicating a time-dependent effect, especially at the highest BZN concentrations tested. This investigation reinforces the potential use of the endemic South American electric fish as a suitable genotoxicity biological model for biomonitoring purposes in the Amazon. Keywords: Comet assay, Micronucleus test, Apteronotus bonapartii, Benzene, Biomonitoring, Electric fish, Amazo

How far is gas from becoming a global commodity?

While we can say that there is a global market for crude oil, we cannot say the same cannot for natural gas. There is a strand of literature that argues that, in the last decades, gas markets have become less regional and more global. We use wavelets to test this hypothesis and conclude otherwise: although the European and Japanese gas markets are signifcantly synchronized, they are much less than the oil markets, which we take as the benchmark. We also show that the North American gas market fluctuations are independent of the other gas markets. Finally, we show that the existing synchronization between gas markets almost vanishes once one filters out the effect of oil price variations, suggesting that it is the global oil market that connects the regional gas markets.Fundação para a Ciência e Tecnologia (FCT). UIDB/03182/2020;UIDP/03182/202

Dieta artificial para criação de Cosmopolites sordidus (Germ.)(Col.: Curculionidae) visando a programas de manejo integrado da praga.

A broca-do-rizoma, Cosmopolites sordidus (Germ.), é considerada a mais severa praga da bananeira, em razão dos prejuízos causados às plantas e da sua ampla distribuição geográfica. Essa praga ocorre em todas as regiões brasileiras e seus danos são causados pelas larvas que abrem galerias no rizoma e base do pseudocaule. O ataque dessa praga, além de reduzir o tamanho dos cachos comprometendo até 50 % da produção, provoca enfraquecimento das plantas, aumento na queda das bananeiras e favorece o ataque do mal-do-Panamá. Considerando a variabilidade genética existente nas populações de C. sordidus e suas implicações na seleção e avaliação de técnicas de controle de pragas, o estabelecimento de um programa de criação da broca-do-rizoma em laboratório é fundamental para o alcance de resultados promissores no manejo integrado do inseto. Este trabalho tem como objetivo determinar diferenças biológicas entre populações de C. sordidus provenientes de regiões produtoras de banana no Brasil e selecionar a(s) dieta(s) mais adequada(s) ao desenvolvimento de populações de C. sordidus em laboratório.PDF. 025

Sympathetic activity in breast cancer and metastasis: partners in crime

The vast majority of patients with advanced breast cancer present skeletal complications that severely compromise their quality of life. Breast cancer cells are characterized by a strong tropism to the bone niche. After engraftment and colonization of bone, breast cancer cells interact with native bone cells to hinder the normal bone remodeling process and establish an osteolytic “metastatic vicious cycle”. The sympathetic nervous system has emerged in recent years as an important modulator of breast cancer progression and metastasis, potentiating and accelerating the onset of the vicious cycle and leading to extensive bone degradation. Furthermore, sympathetic neurotransmitters and their cognate receptors have been shown to promote several hallmarks of breast cancer, such as proliferation, angiogenesis, immune escape, and invasion of the extracellular matrix. In this review, we assembled the current knowledge concerning the complex interactions that take place in the tumor microenvironment, with a special emphasis on sympathetic modulation of breast cancer cells and stromal cells. Notably, the differential action of epinephrine and norepinephrine, through either a- or ß-adrenergic receptors, on breast cancer progression prompts careful consideration when designing new therapeutic options. In addition, the contribution of sympathetic innervation to the formation of bone metastatic foci is highlighted. In particular, we address the remarkable ability of adrenergic signaling to condition the native bone remodeling process and modulate the bone vasculature, driving breast cancer cell engraftment in the bone niche. Finally, clinical perspectives and developments on the use of ß-adrenergic receptor inhibitors for breast cancer management and treatment are discussed.This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and Portuguese funds through FCT/ MCTES in the framework of the project “SproutOC” (POCI-01-0145-FEDER-030158, PTDC/MED-PAT/30158/2017). F.C. is a recipient of the Ph.D. fellowship SFRH/BD/ 128771/2017. D.M.S. is a recipient of the postdoctoral fellowship SFRH/BPD/115341/ 2016