JCV-specific T-cells producing IFN-gamma are differently associated with PmL occurrence in HIV patients and liver transplant recipients

Aim of this work was to investigate a possible correlation between the frequency of JCV-specific T-cells and PML occurrence in HIV-infected subjects and in liver transplant recipients. A significant decrease of JCV-specific T-cells was observed in HIV-PML subjects, highlighting a close relation between JCV-specific T-cell immune impairment and PML occurrence in HIV-subjects. Interestingly, liver-transplant recipients (LTR) showed a low frequency of JCV-specific T-cells, similar to HIV-PML subjects. Nevertheless, none of the enrolled LTR developed PML, suggesting the existence of different immunological mechanisms involved in the maintenance of a protective immune response in LT

Neuromonitoring intraoperatorio del nervo laringeo ricorrente. Un’esperienza di 1000 pazienti consecutivi

Il neuromonitoring intraoperatorio è stato introdotto in chirurgia tiroidea molti anni fa rendendo più semplice l’identificazione del nervo laringeo ricorrente e meno frequenti i danni a suo carico. Tra il 1999 e il 2005 abbiamo raccolto tutti i dati relativi ai pazienti che si sono sottoposti a chirurgia tiroidea e li abbiamo analizzati anno per anno. L’identificazione intraoperatoria del nervo è avvenuta nel 99,2% dei casi (1768 nervi a rischio). La percentuale di emitiroidectomie e tiroidectomie totali è passata dal 17% all’84%. Una minima disfunzione delle corde vocali dovuta a edema o ematoma è stata diagnosticata laringoscopicamente nel 2,9% dei casi. Le paresi permanenti(0,8% nel primo anno di studio) sono progressivamente diminuite fino ad attestarsi allo 0,32% complessivamente. L’introduzione di questa metodica in chirurgia tiroidea è associata a una minore percentuale di paresi del nervo ricorrente anche se la frequenza dei disturbi motori di grado minore non è complessivamente mutata

Physiological responses to pedaling on a water stationary bike at different immersion heights

Only a few studies[1-4] have investigated oxygen consumption (V’O2) and heart rate (HR) responses to pedaling on a stationary bike in water (WSB), while literature is still lacking on the effects elicited by variations in immersion depth. 14 subjects (8 M, 6 F, age 30±6y, weight 67±14kg, BMI 22.8±2.3kg/m2, fat mass 21,4±3%) performed 2 testing sessions and 2 exercise sessions: i) dry-land incremental exercise test (cycle-ergometer) to age-predicted maximum HR (HR); ii) underwater (hip-height) incremental exercise test (on a WSB) to exhaustion; iii/iv) two 2-stage (18-minutes each) underwater pedaling exercises on a WSB (Aqquatix S.r.l., Italy) at 2 different immersion heights (armpit/hip). Breath-by-breath V’O2 and beat-by-beat HR were recorded continuously throughout dry-land sessions. The intensities of the exercise sessions were 45%-55% (stage#1) and 70%-80% (stage#2) of the underwater V’O2 peak (a metronome was set at the V’O2 peak pedaling rate). A repeated-measures (2 exercise intensities) 2-way (armpit/hip heights) ANOVA was performed on each variable and the Bonferroni test was used for post-hoc comparisons. Compared to the hip-height condition, pedaling immersed at the armpit level elicited significantly lower V’O2 and HR (significant solely at high-intensity for HR) responses, either exercising at high (25.5±4.6 vs. 29.1±4.8 mL∙kg-1∙min-1; 127±14 vs. 140±19 beats∙min-1) or low (17.9±3.6 vs. 20.1±3.6 mL∙kg-1∙min-1; 105±16 vs. 110±15 beats∙min-1) intensity. While peak V’O2 didn’t differ significantly between dry-land and underwater tests (36.2±5.4 vs. 38.8±5.8 mL∙kg-1∙min-1), mean HRs at both intensities and both water heights were significantly lower when expressed as percentage of the HR resulting from the underwater test to exhaustion, rather than applying the 220-age prediction equation.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Carbon nanotube four-terminal devices for pressure sensing applications

Carbon nanotubes (CNTs) are of high interest for sensing applications,owing to their superior mechanical strength, high Young’s modulus and low density. In this work, we report on a facile approach for the fabrication of carbon nanotube devices using a four terminal configuration. Oriented carbon nanotube films were pulled out from a CNT forest wafer and then twisted into a yarn. Both the CNT film and yarn were arranged on elastomer membranes/diaphragms which were arranged on a laser cut acrylic frame to form pressure sensors. The sensors were calibrated using a precisely controlled pressure system, showing a large change of the output voltage of approximately 50 mV at a constant supply current of 100 μA and under a low applied pressure of 15 mbar. The results indicate the high potential of using CNT films and yarns for pressure sensing applications

Bacterial Lipopolysaccharide Plus Interferon-γ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells

Abstract Previous results indicate that induction of inducible nitric-oxide synthase (iNOS) expression may be kept suppressed by the endogenous NO level as produced by a constitutive NOS (cNOS) enzyme. In cell types possessing both cNOS and iNOS, this may represent an evident paradox. Here, we report that lipopolysaccharide and interferon-γ, which are able to strongly induce iNOS in astrocytoma cells, can rapidly inhibit the NO production generated by the constitutive NOS isoform, thus obtaining the best conditions for iNOS induction and resolving the apparent paradox. In fact, a 30-min treatment of T67 cells with the combination of lipopolysaccharide plus interferon-γ (MIX) strongly inhibits the cNOS activity, as determined by measuring [3H]citrulline production. In addition, the effect of MIX is also observed by measuring nitrite, the stable breakdown product of NO: a 30-min pretreatment of T67 cells with MIX is able to reduce significantly the N-methyl-D-aspartate-induced nitrite production. Finally, using reverse transcriptase-polymerase chain reaction, we have observed that a 30-min treatment of T67 cells with MIX does not affect expression of mRNA coding for the neuronal NOS-I isoform. These results suggest the novel concept of a possible role of a cNOS isoform in astrocytes as a control function on iNOS induction

How to improve compliance with protective health measures during the covid-19 outbreak. Testing a moderated mediation model and machine learning algorithms

In the wake of the sudden spread of COVID-19, a large amount of the Italian population practiced incongruous behaviors with the protective health measures. The present study aimed at examining psychological and psychosocial variables that could predict behavioral compliance. An online survey was administered from 18–22 March 2020 to 2766 participants. Paired sample t-tests were run to compare efficacy perception with behavioral compliance. Mediation and moderated mediation models were constructed to explore the association between perceived efficacy and compliance, mediated by self-efficacy and moderated by risk perception and civic attitudes. Machine learning algorithms were trained to predict which individuals would be more likely to comply with protective measures. Results indicated significantly lower scores in behavioral compliance than efficacy perception. Risk perception and civic attitudes as moderators rendered the mediating effect of self-efficacy insignificant. Perceived efficacy on the adoption of recommended behaviors varied in accordance with risk perception and civic engagement. The 14 collected variables, entered as predictors in machine learning models, produced an ROC area in the range of 0.82–0.91 classifying individuals as high versus low compliance. Overall, these findings could be helpful in guiding age-tailored information/advertising campaigns in countries affected by COVID-19 and directing further research on behavioral compliance

Autophagy modulation in lymphocytes from COVID-19 patients. new therapeutic target in SARS-COV-2 infection

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the novel coronavirus, causing coronavirus disease 2019 (COVID-19). During virus infection, several pro-inflammatory cytokines are produced, leading to the “cytokine storm.” Among these, interleukin (IL)-6, tumor necrosis factor‐α (TNF‐α), and IL-1β seem to have a central role in the progression and exacerbation of the disease, leading to the recruitment of immune cells to infection sites. Autophagy is an evolutionarily conserved lysosomal degradation pathway involved in different aspects of lymphocytes functionality. The involvement of IL-6, TNF‐α, and IL-1β in autophagy modulation has recently been demonstrated. Moreover, preliminary studies showed that SARS-CoV-2 could infect lymphocytes, playing a role in the modulation of autophagy. Several anti-rheumatic drugs, now proposed for the treatment of COVID-19, could modulate autophagy in lymphocytes, highlighting the therapeutic potential of targeting autophagy in SARS-CoV-2 infection

Natural based products for cleaning copper and copper alloys artefacts

Copper alloys objects can deteriorate their conservation state through irreversible corrosion. Since in the cultural heritage field every artefact is unique and any loss irreplaceable, solutions for conservation are needed. Hence, there is the necessity to stop the corrosion process with a suitable cleaning and conservation process to avoid further degradation processes without changing its morphological aspect. Chelating solutions are commonly used in chemical cleaning, mainly sodium salts of ethylenediaminetetraacetic acid (EDTA). However, it is resistant to water purification procedures and is not biodegradable. The goal of this study was to see if applying an ecologically friendly chelating agent as an alternative to EDTA cleaning procedures for cultural heritage was suitable. In this study were chosen six natural-based chelators that could be a new green non-toxic alternative to EDTA in corrosion-inhibiting properties. They were tested for cleaning copper artefacts exposed to atmospheric environment in polluted areas. The study considered four amino acids, a glucoheptonate (CSA) and an industrial green chelator (GLDA). The effectiveness was tested on corrosion copper compounds and on laboratory corroded copper sheets. Finally, the cleaning efficacy was tested on four Roman coins and a modern copper painting. To define the cleaning efficacy, surface analytical investigations have been carried out by means ICP-OES, UV-VIS, µ-Raman, spectro-colorimetry, XRD and FTIR. Among the amino acids, alanine was the most effective, showing an unaltered noble patina and a good effective copper recovery from corrosion patinas

Rapid inactivation of NOS-I by lipopolysaccharide plus interferon-gamma-induced tyrosine phosphorylation.

Human astrocytoma T67 cells constitutively express a neuronal NO synthase (NOS-I) and, following administration of lipopolysaccharide (LPS) plus interferon-gamma (IFNgamma), an inducible NOS isoform (NOS-II). Previous results indicated that a treatment of T67 cells with the combination of LPS plus IFNgamma, by affecting NOS-I activity, also inhibited NO production in a very short time. Here, we report that under basal conditions, a NOS-I protein of about 150 kDa was weakly and partially tyrosine-phosphorylated, as verified by immunoprecipitation and Western blotting. Furthermore, LPS plus IFNgamma increased the tyrosine phosphorylation of NOS-I, with a concomitant inhibition of its enzyme activity. The same effect was observed in the presence of vanadate, an inhibitor of phosphotyrosine-specific phosphatases. On the contrary, genistein, an inhibitor of protein-tyrosine kinases, reduced tyrosine phosphorylation of NOS-I, enhancing its enzyme activity. Finally, using reverse transcriptase-polymerase chain reaction, we have observed that a suboptimal induction of NOS-II mRNA expression in T67 cells was enhanced by vanadate (or L-NAME) and inhibited by genistein. Because exogenous NO has been found to suppress NOS-II expression, the decrease of NO production that we have obtained from the inactivation of NOS-I by LPS/IFNgamma-induced tyrosine phosphorylation provides the best conditions for NOS-II expression in human astrocytoma T67 cells

Bridging and downstaging to transplantation in HCC

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