53 research outputs found
Carboidratos não estruturais em tubérculos de batata recondicionados após o armazenamento sob diferentes temperaturas
Proteomics analysis suggests broad functional changes in potato leaves triggered by phosphites and a complex indirect mode of action against Phytophthora infestans
Phosphite (salts of phosphorous acid; Phi)-based fungicides are increasingly used in controlling oomycete pathogens, such as the late blight agent Phytophthora infestans. In plants, low amounts of Phi induce pathogen resistance through an indirect mode of action. We used iTRAQ-based quantitative proteomics to investigate the effects of phosphite on potato plants before and after infection with P. infestans. Ninety-three (62 up-regulated and 31 down-regulated) differentially regulated proteins, from a total of 1172 reproducibly identified proteins, were identified in the leaf proteome of Phi-treated potato plants. Four days post-inoculation with P. infestans, 16 of the 31 down-regulated proteins remained down-regulated and 42 of the 62 up-regulated proteins remained up-regulated, including 90% of the defense proteins. This group includes pathogenesis-related, stress-responsive, and detoxification-related proteins. Callose deposition and ultrastructural analyses of leaf tissues after infection were used to complement the proteomics approach. This study represents the first comprehensive proteomics analysis of the indirect mode of action of Phi, demonstrating broad effects on plant defense and plant metabolism. The proteomics data and the microscopy study suggest that Phi triggers a hypersensitive response that is responsible for induced resistance of potato leaves against P. infestans. Biological significance: Phosphie triggers complex functional changes in potato leaves that are responsible for the induced resistance against Phytophthora infestans.This article is part of a Special Issue entitled: Translational Plant Proteomics. \ua9 2013 Elsevier B.V.Peer reviewed: YesNRC publication: Ye
The effect of scion and stock autografting and heterografting on specific gravity, sugar content and chip color of potatoes
Saginaw Gold: A yellow-fleshed potato cultivar with medium-high specific gravity and excellent chip and french fry quality after storage
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Experience of TSTA (Tritium Systems Test Assembly) milestone runs with 100 grams-level of tritium
The first loop operation tests of the Tritium Systems Test Assembly (TSTA) with 100 grams-level of tritium were performed at the Los Alamos National Laboratory (LANL) in June and July, 1987. The July run was resumption of the June run, which was halted because of a loss of cryogenic refrigerant in the hydrogen isotope separation system. 3 refs., 3 figs
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