Transforming growth factor-β1 enhances the interferon-γ-dependent, interleukin-12-independent pathway of T helper 1 cell differentiation

Transforming growth factor (TGF)-β, a pleiotropic cytokine that has multiple effects on immune responses, has been shown to inhibit interleukin (IL)-4/GATA-3 expression as well as T helper 2 (Th2) differentiation. Consistent with these reports, we found that priming T cells from DO11.10 transgenic mice with antigen in the presence of TGF-β inhibited GATA-3 expression and the development of IL-4-producing T cells. Unexpectedly, the inhibition of Th2 development was accompanied by a substantial increase in the number of interferon-γ (IFN-γ)-producing cells. T cells primed with TGF-β secreted IFN-γ in response to both T-cell receptor ligation and IL-12/IL-18 stimulation, and expressed high levels of T-bet and low levels of GATA-3. The TGF-β-mediated enhancement of T helper 1 (Th1) priming was independent of IL-12 and signal transducer and activator of transcription (STAT)-4, but required endogenous IFN-γ. TGF-β-mediated enhancement of the IFN-γ-dependent, IL-12-independent pathway of Th1 priming was mediated primarily by the inhibition of IL-4 produced by memory/activated T cells in the unfractionated CD4(+) responder population. Nevertheless, TGF-β did not inhibit this pathway of Th1 differentiation when purified naive CD4(+) T cells were used as responders. These data have important implications for strategies being considered for the use of TGF-β-producing T cells for the treatment of autoimmune disorders