Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

C–H bonds are ubiquitous structural units of organic molecules. Although these bonds are generally considered to be chemically inert, the recent emergence of methods for C–H functionalization promises to transform the way synthetic chemistry is performed. The intermolecular amination of C–H bonds represents a particularly desirable and challenging transformation for which no efficient, highly selective, and renewable catalysts exist. Here we report the directed evolution of an iron-containing enzymatic catalyst—based on a cytochrome P450 monooxygenase—for the highly enantioselective intermolecular amination of benzylic C–H bonds. The biocatalyst is capable of up to 1,300 turnovers, exhibits excellent enantioselectivities, and provides access to valuable benzylic amines. Iron complexes are generally poor catalysts for C–H amination: in this catalyst, the enzyme's protein framework confers activity on an otherwise unreactive iron-haem cofactor

Design and engineering of cytochrome P450 systems

Stephen G. Bell, Nicola Hoskins, Christopher J. C. Whitehouse and Luet L. Won

Work–life programs and performance in Australian organisations: the role of organisation size and industry type

Organisations are increasingly using work–life programs to strategically manage their workforce in a competitive labour market. Extant research has investigated various outcomes of work–life programs but has lacked focus on organisational financial performance and context. Drawing on strategic human resource management theory, this study proposes and tests a work–life programs–performance relationship. It also investigates the moderating effects of organisation size and industry on the work–life programs–performance relationship. We used a time-lagged design and data from multiple sources to link work–life programs with firm performance in 117 organisations in Australia. The findings support the hypothesis that work–life programs are positively associated with financial performance. This study also provides pioneering evidence for the moderating effects of organisation size and industry type on the work–life programs–organisational performance relationship.</p