Detection of Genetic Variations Using RAPD Markers in Siberian Huskies Affected with Swimming Puppy Syndrome บทคั ดย่ อ การตรวจสอบความหลากหลายทางพั นธุ กรรมของกลุ ่ มอาการขากางในลู กสุ นั ข ในสุ นั ขสายพั นธุ ์ ไซบี เรี ยนฮั สกี ด้ วยเทคนิ ค RAPD marker ก

Abstract Swimming puppy syndrome (SPS) is a curious disease found in dogs. The causes and pathology of this disease are still unknown, although heredity is usually considered to be one of the underlying factors. The objective of this study was to investigate the association between genetics and SPS in a Siberian husky model. Four Siberian husky puppies diagnosed with SPS were subjects of this study, while three other healthy Siberian husky puppies served as controls. Blood samples were collected for DNA isolation, using random amplified polymorphic DNA (RAPD) technique with 16 random primers. No genetic variation was found between affected puppies and healthy puppies, which indicated that swimming puppy syndrome is not controlled by genetics

Phylogenetic partitioning of the third-largest vertebrate genus in the world, Cyrtodactylus Gray, 1827 (Reptilia; Squamata; Gekkonidae) and its relevance to taxonomy and conservation

10.3897/vertebrate-zoology.71.e59307Vertebrate Zoology71101-15

Phylogenetic partitioning of the third-largest vertebrate genus in the world, Cyrtodactylus Gray, 1827 (Reptilia; Squamata; Gekkonidae) and its relevance to taxonomy and conservation

The gekkonid genus Cyrtodactylus is the third most speciose vertebrate genus in the world, containing well over 300 species that collectively range from South Asia to Melanesia across some of the most diverse landscapes and imperiled habitats on the planet. A genus-wide phylogeny of the group has never been presented because researchers working on different groups were using different genetic markers to construct phylogenies that could not be integrated. We present here Maximum likelihood and Bayesian inference mitochondrial and mito-nuclear phylogenies incorporating of 310 species that include dozens of species that had never been included in a genus-wide analysis. Based on the mitochondrial phylogeny, we partition Cyrtodactylus into 31 well-supported monophyletic species groups which, if used as recommended herein, will increase the information content of future integrative taxonomic analyses that continue to add new species to this genus at an ever-increasing annual rate. Data presented here reiterate the outcome of several previous studies indicating that Cyrtodactylus comprises an unprecedented number of narrow-range endemics restricted to single mountain tops, small islands, or karst formations that still remain unprotected. This phylogeny can provide a platform for various comparative ecological studies that can be integrated with conservation management programs across the broad diversity of landscapes and habitats occupied by this genus. Additionally, these data indicate that the true number of Cyrtodactylus remains substantially underrepresented