13 research outputs found
Evaluation of a new amplified enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in male urine, female endocervical swab, and patient obtained vaginal swab specimens
AimsâTo compare the performance of a new generation dual amplified enzyme immunoassay (EIA) with a molecular method for the diagnosis of Chlamydia trachomatis, using a range of urogenital samples, and to assess the reliability of testing self collected vaginal specimens compared with clinician collected vaginal specimens. MethodsâTwo population groups were tested. For the first population group, first void urine samples were collected from 193 male patients with urethritis, and endocervical swabs were collected from 187 high risk commercial sex workers. All urine and endocervical specimens were tested by a conventional assay (IDEIA chlamydia), a new generation amplified immunoassay (IDEIA PCE chlamydia), and the Amplicor polymerase chain reaction (PCR). Discrepant results obtained among the three sample types were confirmed using a nested PCR test with a different plasmid target region. For the second population group, four swab specimens, including one patient obtained vaginal swab, two clinician obtained endocervical swabs, and one clinician obtained vaginal swab, were collected from 91 high risk sex workers. Self collected and clinician collected vaginal swabs were tested by IDEIA PCE chlamydia. Clinician obtained endocervical swabs were assayed by IDEIA PCE chlamydia and Amplicor PCR. ResultsâThe performance of the IDEIA PCE chlamydia test was comparable to that of the Amplicor PCR test when male urine and female endocervical swab specimens were analysed. The relative sensitivities of IDEIA, IDEIA PCE, and Amplicor PCR on male first void urine specimens were 79.3%, 91.4%, and 100%, respectively. The relative sensitivities of the three tests on female endocervical specimens were 85.0%, 95.0%, and 100%, respectively. The positivity rates for patient collected vaginal specimens and clinician collected vaginal specimens by IDEIA PCE were 25.2% and 23.1%, respectively, whereas those for clinician collected endocervical swabs by PCR and IDEIA PCE were both 27.5%. ConclusionsâIDEIA PCE chlamydia is a lower cost but sensitive alternative test to PCR for testing male urine samples and female endocervical swabs. In addition, self collected or clinician collected vaginal specimens tested by IDEIA PCE chlamydia are a reliable alternative to analysing endocervical specimens. Key Words: Chlamydia trachomatis âą enzyme immunoassay âą clinical specimen
Influence of storing urogenital specimens at -20 degrees C before testing by enzyme amplified immunoassay (IDEIA) to detect Chlamydia trachomatis antigen.
The inhibitory effect of phosphate on the ligase chain reaction used for detecting Chlamydia trachomatis
Leukocyte esterase urine strips for the screening of men with urethritis--use in developing countries.
Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens.
Association between human parvovirus B19 and arthropathy in BelĂ©m, ParĂĄ, north Brazil Associação entre parvovĂrus B19 e artropatias em BelĂ©m, ParĂĄ, norte do Brasil
A total of 220 patients with arthropathy were selected in BelĂ©m, ParĂĄ between January 1994 and December 2000, and screened for the presence of human parvovirus B19 IgM and IgG antibodies by enzyme-linked immunosorbent assay (ELISA). A subgroup (n = 132) of patients with high levels of antibodies (either IgM+/IgG+ or IgM-/IgG+) were examined for the presence of DNA by polymerase chain reaction/nested PCR. Recent/active infection (detection of IgM and/or IgG-specific antibodies and presence of viral DNA) was identified in 47.7% of the 132 individuals with arthropathy. In our study, women were significantly more affected (59.7%) than men (35.4%) (P = 0.0006). The age group of 11-20 years (84.6%), among female patients, and 21-30 years (42.1%), among male, were those with the highest incidence rates. The analysis of the temporal distribution of B19-associated arthropaties showed a cyclic pattern, with peak incidence rates occuring at 3-5 year intervals. Significant diference (P = 0.01) was observed when comparing both the highest (39.0%) and the lowest (11.0%) seropositivity rates for the years of 1995 and 2000, respectively. The interfalangial joints of hands and feet were mostly affected, with 50.0% and 48.0% of cases among both women and men, respectively. In a smaller proportion, other joints such as those of knee, ankle, pulse and shoulder were affected. As for the duration, symptoms lasted 1 to 5 days in 54.0% of the individuals, whereas in 46.0% of them the disease lasted 6-10 days, if considered the subgroup (n = 63) of patients with recent/active infection by parvovirus B19. In our study, joint clinical manifestations occurred symmetrically. Our results indicate that B19 may be an important agent of arthropathies in our region, and this underscores the need for specific laboratory diagnosis when treating patients suffering from acute arthropathy, mainly pregnant women.<br>Um total de 220 indivĂduos portadores de artropatias foi selecionado em BelĂ©m, ParĂĄ, entre janeiro de 1994 e dezembro de 2000 e, posteriormente, examinado com o propĂłsito de se detectarem anticorpos IgM e IgG para o parvovĂrus B19, utilizando-se a tĂ©cnica imunoenzimĂĄtica (ELISA). Um subgrupo (n = 132) de indivĂduos com amostras de soro apresentando altos nĂveis de anticorpos (IgM+/IgG+ e IgM-/IgG+) foi usado para detecção de DNA do B19 atravĂ©s da reação em cadeia da polimerase (PCR) e do "nested" PCR. Infecção recente/ativa (detecção de IgM e/ou IgG mais a presença de DNA viral) foi diagnosticada em 47,7% dos 132 indivĂduos apresentando comprometimento das articulaçÔes. O sexo feminino foi mais afetado (59,7%) que o masculino (35,4%), com diferença estatisticamente significativa (P = 0,0006). Os grupos etĂĄrios mais atingidos foram os de 11-20 anos (84,6%), no sexo feminino, e 21-30 anos (42,1%), no masculino. A anĂĄlise da distribuição temporal mostrou um padrĂŁo cĂclico, com perĂodos de maior e menor atividade viral que variam de 3 a 5 anos. Diferença estatisticamente significativa (P = 0,01) foi observada quando comparadas as freqĂŒĂȘncias de positividade mais alta (39,0%) e mais baixa (11,0%) para os anos de 1995 e 2000, respectivamente. As articulaçÔes mais atingidas foram, em ordem de freqĂŒĂȘncia, as interfalangianas de mĂŁos e pĂ©s, com 50,0% e 48,0% para o sexo feminino e masculino, respectivamente. Em menor proporção outras articulaçÔes tais como as do joelho, tornozelo, pulso e ombro foram afetadas. Quanto Ă duração das manifestaçÔes articulares, 54,0% evoluĂram por 1-5 dias, e 46,0% ao longo de 6-10 dias, considerando o subgrupo (n = 63) de indivĂduos com infecção recente/ativa para o B19 em ambos os sexos. Em nosso estudo, o comprometimento das articulaçÔes apresentou carĂĄter simĂ©trico. Os resultados encontrados demonstraram o freqĂŒente acometimento articular associado Ă s infecçÔes recentes/ativas por parvovĂrus B19, ressaltando a necessidade do diagnĂłstico laboratorial dessa virose, principalmente entre gestantes