Análise de pureza genética de sementes de feijoeiro comum utilizando marcadores microssatélites em sistema de genotipagem multiplex.

Esse estudo teve como objetivo avaliar quanto a pureza genética um conjunto de sementes de feijão comum resultantes do processo de multiplicação da cultivar BRS Campeiro, incluindo as sementes genéticas, sementes básicas e sementes com tipo diferenciado de grãos, quando comparados com a cultivar padrão, utilizando um sistema de genotipagem semiautomatizado baseado em marcadores microssatélites.bitstream/CNPAF/26677/1/bolpesq_28.pd

Correlation of exhaled breath temperature with bronchial blood flow in asthma

In asthma elevated rates of exhaled breath temperature changes (Δe°T) and bronchial blood flow (Q(aw)) may be due to increased vascularity of the airway mucosa as a result of inflammation. We investigated the relationship of Δe°T with Q(aw )and airway inflammation as assessed by exhaled nitric oxide (NO). We also studied the anti-inflammatory and vasoactive effects of inhaled corticosteroid and β(2)-agonist. Δe°T was confirmed to be elevated (7.27 ± 0.6 Δ°C/s) in 19 asthmatic subjects (mean age ± SEM, 40 ± 6 yr; 6 male, FEV(1 )74 ± 6 % predicted) compared to 16 normal volunteers (4.23 ± 0.41 Δ°C/s, p < 0.01) (30 ± 2 yr) and was significantly increased after salbutamol inhalation in normal subjects (7.8 ± 0.6 Δ°C/ s, p < 0.05) but not in asthmatic patients. Q(aw), measured using an acetylene dilution method was also elevated in patients with asthma compared to normal subjects (49.47 ± 2.06 and 31.56 ± 1.6 μl/ml/min p < 0.01) and correlated with exhaled NO (r = 0.57, p < 0.05) and Δe°T (r = 0.525, p < 0.05). In asthma patients, Q(aw )was reduced 30 minutes after the inhalation of budesonide 400 μg (21.0 ± 2.3 μl/ml/min, p < 0.05) but was not affected by salbutamol. Δe°T correlates with Q(aw )and exhaled NO in asthmatic patients and therefore may reflect airway inflammation, as confirmed by the rapid response to steroids

Identification and mutation analysis of the complete gene for Chediak-Higashi syndrome

Chediak-Higashi syndrome (CHS) is a rare, autosomal recessive disorder characterized by hypopigmentation, severe immunologic deficiency with neutropenia and lack of natural killer (NK) cells, a bleeding tendency and neurologic abnormalities1–4. Most patients die in childhood. The CHS hallmark is the occurrence of giant inclusion bodies and organelles in a variety of cell types, and protein sorting defects into these organelles5–8. Similar abnormalities occur in the beigemouse6,7,9–13, the proposed model for human CHS. Two groups have recently reported the identification of the beige gene14,15, however the two cDNAs were not at all similar. Here we describe the sequence of a human cDNA homologous to mouse beige, identify pathologic mutations and clarify the discrepancies of the previous reports. Analysis of the CHS polypeptide demonstrates that its modular architecture is similar to the yeast vacuolar sorting protein, VPS15

Evaluation of a flexible fiberoptic catheter in confirming endotracheal tube placement in the intensive care unit

UNLABELLEDWe evaluated the use of an inexpensive 2.2-mm fiberoptic catheter (FOC) to assess endotracheal tube (ETT) placement in 22 critically ill adult patients.METHODThe distance between the carina and the distal tip of the ETT was measured under direct visualization with the FOC and compared to the corresponding measurement obtained from concomitant chest radiographs.RESULTSThere was a significant linear correlation between measurements made by the two methods (r = 0.97, P less than 0.0001), and all FOC measurements were within 1 cm of the corresponding radiograph measurements. No complications or significant changes occurred in peak airway pressure, tidal volume, respiratory rate, or heart rate. The procedure usually was performed in less than 1 minute. Cost analysis revealed the possibility of major savings when the FOC is used instead of portable chest radiography.CONCLUSIONSWe conclude that documentation of endotracheal tube placement by means of a fiberoptic catheter is rapid, accurate, safe, and cost-effective

Airway mucosal blood flow: response to autonomic and inflammatory stimuli

A major portion of airway blood flow is distributed to the subepithelial tissue space and is strategically located to influence both epithelial and airway smooth muscle functions. To assess the magnitude and responsiveness of blood flow through the subepithelial microvasculature, we measured tracheal mucosal blood flow with a soluble gas method in intact sheep. We found responses of tracheal mucosal blood flow to pharmacological stimuli alpha- and beta- (adrenoceptor agonists) and inflammatory stimuli (antigen and histamine), and demonstrated that alterations in mucosal blood flow influence the magnitude and duration of allergic airway smooth muscle contraction in the trachea. Mucosal blood flow, which under certain circumstances is regulated independently of total airway blood flow, could play a critical role in the manifestations of and recovery from airway disease