HYALURONAN-A NOVEL POLYMER ISOLATED FROM MUTATED CLINICAL BACTERIAL ISOLATE

Objective: This study was done to optimize the production parameters involved in the isolation of hyaluronan (HA) from UV mutated a clinical strain of Klebsiella pneumonia (M 3020).Methods: Glucose and nitrogen enriched media (D-glucose, L-glutamic acid, and peptone) were utilized to cultivate the clinical isolate K. pneumoniae. The strain was Ultra Violet (UV) radiation mutated (254 nm, 25 min) and HA production was optimized by parameters such as pH and temperature. The isolated HA from the fermented broth was subjected to purification by isopropyl alcohol and silica gel and further dried by lyophilization. Produced HA was confirmed with UV and Fourier Transform Infra-Red (FT-IR) spectroscopy.Results: UV treated strain at 254 nm for 25 min predominantly produce a high quantity of HA (3.5 g/l) in 37 °C, 300 rpm and pH 6.8 at 24 h run. UV and IR spectrum of produced HA showed strong similarity with the standard hyaluronan.Conclusion: To conclude, high quantity and quality of HA can be isolated from mutated clinical strains of K. pneumoniae

A STUDY ON DIFFERENT PELLET FORMATION TECHNIQUES AND ITS EVALUATION PARAMETERS-A REVIEW

This review article deals with the various pelletization techniques utilized in the pharmaceutical industry for spheroidal particle production i.e., pellet for mainly oral administration which can be further formulated into several other dosage forms such as tablets, capsules or can be administered as such. Now-a-days oral administration has become the most versatile, convenient and common route of drug administration which ultimately focuses on patient compliance. The technique which is setting horizon in pelletization is “Extrusion Spheronization” because of its simple and easy steps involved in pellet production in a faster way. This review also includes the characterization and evaluation of pellets to ensure its quality, safety and efficacy to give out the required therapeutic activity after administration

HYALURONAN-A NOVEL POLYMER ISOLATED FROM MUTATED CLINICAL BACTERIAL ISOLATE

Objective: This study was done to optimize the production parameters involved in the isolation of hyaluronan (HA) from UV mutated a clinical strain of Klebsiella pneumonia (M 3020).Methods: Glucose and nitrogen enriched media (D-glucose, L-glutamic acid, and peptone) were utilized to cultivate the clinical isolate K. pneumoniae. The strain was Ultra Violet (UV) radiation mutated (254 nm, 25 min) and HA production was optimized by parameters such as pH and temperature. The isolated HA from the fermented broth was subjected to purification by isopropyl alcohol and silica gel and further dried by lyophilization. Produced HA was confirmed with UV and Fourier Transform Infra-Red (FT-IR) spectroscopy.Results: UV treated strain at 254 nm for 25 min predominantly produce a high quantity of HA (3.5 g/l) in 37 °C, 300 rpm and pH 6.8 at 24 h run. UV and IR spectrum of produced HA showed strong similarity with the standard hyaluronan.Conclusion: To conclude, high quantity and quality of HA can be isolated from mutated clinical strains of K. pneumoniae

Formulation and evaluation of ocular drops containing solid dispersion of tobramycin

Tobramycin is a potent antimicrobial aminoglycoside that can be used to treat ocular infections. Its solubility and bioavailability are limited, so its solid dispersion was prepared using PVP-K30, HPMC and Beta cyclodextrin by solvent evaporation technique. This solid dispersion was formulated as an eye drop using sodium chloride, EDTA and benzalkonium chloride. Totally 9 formulations were prepared. The in-vitro dissolution profile of optimised formulation (F6) showed 85.1% drug release at the end of 2nd hour. The optimized formula was evaluated which showed no microbial growth, and drug content uniformity was 80.12% to 85%. The optimized formulation was evaluated for clarity, pH, isotonicity, microbial growth, stability and in vitro diffusion studies and all these showed acceptable results

FAST DISSOLVING ORAL FILMS: A NOVEL DRUG DELIVERY SYSTEM FOR DICLOFENAC

ABSTRACT are the most advanced form of oral solid dosage form due to more flexibility and comfort. It improves the efficacy of the API's by dissolving within a minute in oral cavity after the contact with less saliva as compared to Fast Dissolving Tablets (FDT's), without chewing and no need of water for administration. In the present study fast dissolving film of Diclofenac sodium was prepared by solvent casting method using sodium alginate as film forming polymer and kollidon as a superdisintegrant. The prepared films were evaluated for their morphology, thickness, folding endurance, drug content, in-vitro disintegration and dissolution studies. The microphotographs of SEM indicated that a very homogenous and smooth surface of the polymers with uniform dispersion of drug. The drug content increased with increased kollidon ratio and decreased sodium alginate ratio. Increased kollidon ratio led to decreased disintegration time due to its insoluble nature and had no effect on sodium alginate ratio. The release rate increased with increase in kollidon ratio and decreased by the addition of sodium alginate. Formulation F 3 showed fast and maximum release which was taken as a best formulation. Thus the fast dissolving films of Diclofenac sodium can be prepared by solvent technique using sodium alginate as film base and kollidon as superdisintegrant