5 research outputs found
Transgenic mouse model overexpressing spermine oxidase (SMO) to investigate the neurobiolological role of polyamine metabolism in physiological and pathological conditions
Construction of an enginereed mouse model over-expressing the homologous mouse spermine oxidase (mSMO) isoform
Effects of overexpression of spermine oxidase (SMO) in kainic acid induced excitotoxicity
Maize polyamine oxidase: primary structure from protein and cDNA sequencing
The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis. A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined. This cDNA contains information for a polypeptide chain of 500 amino acids. Its amino-terminal sequence shows the typical features of secretion signal peptides. The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin-containing monoamine oxidases are also discussed