18 research outputs found
Reproducibility of adipogenic responses to metabolism disrupting chemicals in the 3T3-L1 pre-adipocyte model system: An interlaboratory study
The 3T3-L1 murine pre-adipocyte line is an established cell culture model for screening Metabolism Disrupting Chemicals (MDCs). Despite a need to accurately identify MDCs for further evaluation, relatively little research has been performed to comprehensively evaluate reproducibility across laboratories, assess factors that might contribute to varying degrees of differentiation between laboratories (media additives, plastics, cell source, etc.), or to standardize protocols. As such, the goals of this study were to assess interlaboratory variability of efficacy and potency outcomes for triglyceride accumulation and pre-adipocyte proliferation using the mouse 3T3-L1 pre-adipocyte cell assay to test chemicals. Ten laboratories from five different countries participated. Each laboratory evaluated one reference chemical (rosiglitazone) and three blinded test chemicals (tributyltin chloride, pyraclostrobin, and bisphenol A) using: 1) their Laboratory-specific 3T3-L1 Cells (LC) and their Laboratory-specific differentiation Protocol (LP), 2) Shared 3T3-L1 Cells (SC) with LP, 3) LC with a Shared differentiation Protocol (SP), and 4) SC with SP. Blinded test chemical responses were analyzed by the coordinating laboratory. The magnitude and range of bioactivities reported varied considerably across laboratories and test conditions, though the presence or absence of activity for each tested chemical was more consistent. Triglyceride accumulation activity determinations for rosiglitazone ranged from 90 to 100% across test conditions, but 30â70 % for pre-adipocyte proliferation; this was 40â80 % for triglyceride accumulation induced by pyraclostrobin, 80â100 % for tributyltin, and 80â100 % for bisphenol A. Consistency was much lower for pre-adipocyte proliferation, with 30â70 % active determinations for pyraclostrobin, 30â50 % for tributyltin, and 20â40 % for bisphenol A. Greater consistency was observed for the SC/SP assessment. As such, working to develop a standardized adipogenic differentiation protocol represents the best strategy for improving consistency of adipogenic responses using the 3T3-L1 model to reproducibly identify MDCs and increase confidence in reported outcomes.Over-arching project supported by grants [R01 ES016099 to HMS; R00 ES030405 to CDK] from the National Institute of Environmental Health Sciences (NIEHS); University of Turin; European Union's Horizon 2020 research and innovation program under grant agreement GOLIATH No. 825489; Brunel University London; NIEHS (1K22ES026208 and R01ES027863); NIEHS (Z0ES102785); Spanish Institute of Health Carlos III (grant FIS-PI16/01812)
Impacts of food contact chemicals on human health: a consensus statement
Food Packaging Forum Foundation (FPF) and the Plastics Solution Fund (PSF
Effects of prenatal and lactational bisphenol a and/or di(2-ethylhexyl) phthalate exposure on male reproductive system
Recommended from our members
Systematic evidence on migrating and extractable food contact chemicals: Most chemicals detected in food contact materials are not listed for use
Food packaging is important for todayâs globalized food system, but food contact materials (FCMs)
can also be a source of hazardous chemicals migrating into foodstuffs. Assessing the impacts of
FCMs on human health requires a comprehensive identification of the chemicals they contain, the
food contact chemicals (FCCs). We systematically compiled the âdatabase on migrating and extractable
food contact chemicalsâ (FCCmigex) using information from 1210 studies. We found that to date
2881 FCCs have been detected, in a total of six FCM groups (Plastics, Paper & Board, Metal,
Multi-materials, Glass & Ceramic, and Other FCMs). 65% of these detected FCCs were previously not
known to be used in FCMs. Conversely, of the more than 12â000 FCCs known to be used, only 1013
are included in the FCCmigex database. Plastic is the most studied FCM with 1975 FCCs detected.
Our findings expand the universe of known FCCs to 14,153 chemicals. This knowledge contributes
to developing non-hazardous FCMs that lead to safer food and support a circular economy.Sympany Foundation, MAVA Foundation,
and Fondation Valery, as well as by the Food Packaging Forumâs (FPF)
own resources from unrestricted donations. All FPF funding sources
are listed https://www.foodpackagingforum.org/about-us/funding. AO
and VNS received funding from the Federal Food Safety and Veterinary
Office, grant 4.21.0