Assembly, organization, and function of the COPII coat

A full mechanistic understanding of how secretory cargo proteins are exported from the endoplasmic reticulum for passage through the early secretory pathway is essential for us to comprehend how cells are organized, maintain compartment identity, as well as how they selectively secrete proteins and other macromolecules to the extracellular space. This process depends on the function of a multi-subunit complex, the COPII coat. Here we describe progress towards a full mechanistic understanding of COPII coat function, including the latest findings in this area. Much of our understanding of how COPII functions and is regulated comes from studies of yeast genetics, biochemical reconstitution and single cell microscopy. New developments arising from clinical cases and model organism biology and genetics enable us to gain far greater insight in to the role of membrane traffic in the context of a whole organism as well as during embryogenesis and development. A significant outcome of such a full understanding is to reveal how the machinery and processes of membrane trafficking through the early secretory pathway fail in disease states

Development and validation of an instrument for assessing high-school students’ perceptions of socio-scientific issues-based learning in biology

© 2020, Springer Nature B.V. In this research, we developed and validate an instrument for assessing high-school students’ perceptions of socio-scientific issues (SSI)-based learning in biology. The development of the instrument was carried out using the following four steps: item formulation, content validation, construct validation and reliability calculation. According to an extensive literature review, 28 initial items were formulated for four SSI-based instruction aspects: (1) contextualisation of SSI, (2) student involvement, (3) student attitudes towards SSI-learning and (4) SSI-based learning objectives. Based on the pilot study conducted for construct validation, 24 items were successfully constructed for the four scales and subsequently administered in biology classrooms to 151 students. Factor analysis showed that each of the 24 items had a factor loading at least 0.40 on its own scale and no other scale. Moreover, the four scales accounted for 58.32% of the total variance and the alpha reliability coefficients for the scales ranged from 0.75 to 0.85. According to these findings, the SSI-based Learning Environment Inventory in Biology is valid, reliable and useful