543 research outputs found
Actinopterygii, Characiformes, Characidae: Ectrepopterus uruguayensis (Fowler, 1943): New record for Argentina
Ectrepopterus uruguayensis Fowler was recently redescribed, with occurrence in lower tributaries of Río de la Plata and lower Río Uruguay, Uruguay. Herein its occurrence is extended and confirmed to Argentina, arroyo El Molino, in Uruguay Department, Entre Ríos Province, Uruguay drainage.Fil: Miquelarena, Amalia Maria. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico Zoología Vertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carvalho, Fernando R.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasi
Actinopterygii, Characiformes, Characidae: <i>Ectrepopterus uruguayensis</i> (Fowler, 1943): New record for Argentina
The monotypic genus Ectrepopterus Fowler was recently resurrected by Malabarba et al. (2012) for Megalamphodus uruguayensis Fowler, a small-sized fish (up to 47.4 mm SL) which habit the Uruguayan drainages (figure 7 of Malabarba et al. 2012). Diagnostic characters for genus include: i) an incomplete foramen in posterior region of metapterygoid, that serves as passage for the ramus mandibularis of the trigeminus nerve, bordered posteriorly by the hyomandibula and forming an incomplete arch; ii) pectoral-fin rays bearing hooks; iii) posterior margin of second infraorbital posteroventrally oblique and second infraorbital ventrally bordering anterior region of third infraorbital; iv) fourth infraorbital more developed longitudinally than dorsoventrally; v) ascending process of premaxilla reaching just anterior end of nasal, vi) and lateral line interrupted (Malabarba et al. 2012).Facultad de Ciencias Naturales y Muse
Interest of 3-arylcoumarins as xanthine oxidase inhibitors
The 19th International Electronic Conference on Synthetic Organic Chemistry session Bioorganic, Medicinal and Natural Products ChemistryIn the current paper we studied the interest of a series of 3-arylcoumarin derivatives as xanthine oxidase inhibitors. For the best compound of the series, the 4’-methoxyphenyl-6-nitrocoumarin, it was determined the IC50 value and the type of inhibition. This work is a preliminary screening for further design and synthetize new non-purinergic derivatives as potential compounds involved in the inflammatory suppression, specially related to the gou
Gambiarra também é Design? Uma abordagem sistêmica do Design acadêmico e o vernacular
Academic Design is understood as that studied in universities, and involves the pillars of teaching, research and extension, while vernacular Design, in this work also found as gambiarra, elaboration through the cultural knowledge acquired in the experiences of the population. Thus, through a holistic view, that is, a systemic approach including the terms academic design and vernacular design, which are pointed out as opposites, it is intended to identify the main congruences between them. The methodology was composed of bibliographical research, and action-research oriented to Design, in which the initial stages of recognizing and diagnosing were worked. Sixteen publications were analyzed, and as a result it was possible to understand that academic design and vernacular design are intrinsically connected, mainly through the areas of social innovation and sustainability.O Design acadêmico é compreendido como aquele estudado em universidades, e envolve os pilares de ensino, pesquisa e extensão, já o Design vernacular, neste trabalho também encontrado como gambiarra, engloba a elaboração do Design por meio do conhecimento cultural adquirido nas vivências da população. Desta forma, por meio de uma visão holística, ou seja, uma abordagem sistêmica incluindo os termos Design acadêmico e Design vernacular, os quais são apontados como opostos, pretende-se identificar as principais congruências entre eles. A metodologia foi composta pela pesquisa bibliográfica, e a pesquisa-ação orientada para o Design, em que foram trabalhadas as etapas iniciais de reconhecer e diagnosticar. Foram analisadas 16 publicações, e como resultado foi possível compreender que o Design acadêmico e o Design vernacular estão intrinsecamente conectados, principalmente por meio das áreas de inovação social e sustentabilidade
Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
Background: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. in this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes.Results: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. in the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR.Conclusion: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. the distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. the presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, BR-31270901 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Programa Posgrad Bioinformat, BR-31270901 Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilUniv Brasilia, Inst Ciencias Biol, Lab Biol Mol, BR-70910900 Brasilia, DF, BrazilUniv Fed Goias, Inst Ciencias Biol, Lab Biol Mol, BR-74001970 Goiania, Go, BrazilFIOCRUZ Minas, Ctr Pesquisas Rene Rachou, Grp Informat Biossistemas, BR-30190002 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Dept Biol Geral, BR-31270901 Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, BrazilFAPEMIG: APQ-01661-13CNPq: 301652/2012-0CNPq: 486618/2013-7Web of Scienc
Effect of amylose:amylopectin ratio and rice bran addition on starchfilms properties
The influence of the amylose:amylopectin ratio on the properties of pea, potato and cassava starch films and the effect of the incorporation of rice bran of two different particle sizes were studied. The structural, mechanical, optical and barrier properties of the films were analyzed after 1 and 5 weeks. The high content of amylose gave rise to stiffer, more resistant to fracture, but less stretchable films, with lower oxygen permeability and greater water binding capacity. Although no changes in the water vapour permeability values of the films were observed during storage, their oxygen permeability decreased. Throughout storage, films became stiffer, more resistant to break, but less stretchable. Rice bran with the smallest particles improved the elastic modulus of the films, especially in high amylose content films, but reduced the film stretchability and its barrier properties, due to the enhancement of the water binding capacity and the introduction of discontinuities.The authors acknowledge the financial support from the Spanish Ministerio de Economia y Competitividad throughout the project AGL2010-20694, co-financed with FEDER founds. Amalia Cano also thanks Spanish Ministerio de Educacion, Cultura y Deporte for the FPU grant.Cano Embuena, AI.; Jiménez Marco, A.; Cháfer Nácher, MT.; González Martínez, MC.; Chiralt Boix, MA. (2014). Effect of amylose:amylopectin ratio and rice bran addition on starchfilms properties. Carbohydrate Polymers. 111:543-555. https://doi.org/10.1016/j.carbpol.2014.04.075S54355511
Clustering COVID-19 ARDS patients through the first days of ICU admission. An analysis of the CIBERESUCICOVID Cohort
Background Acute respiratory distress syndrome (ARDS) can be classified into sub-phenotypes according to different inflammatory/clinical status. Prognostic enrichment was achieved by grouping patients into hypoinflammatory or hyperinflammatory sub-phenotypes, even though the time of analysis may change the classification according to treatment response or disease evolution. We aimed to evaluate when patients can be clustered in more than 1 group, and how they may change the clustering of patients using data of baseline or day 3, and the prognosis of patients according to their evolution by changing or not the cluster.Methods Multicenter, observational prospective, and retrospective study of patients admitted due to ARDS related to COVID-19 infection in Spain. Patients were grouped according to a clustering mixed-type data algorithm (k-prototypes) using continuous and categorical readily available variables at baseline and day 3.Results Of 6205 patients, 3743 (60%) were included in the study. According to silhouette analysis, patients were grouped in two clusters. At baseline, 1402 (37%) patients were included in cluster 1 and 2341(63%) in cluster 2. On day 3, 1557(42%) patients were included in cluster 1 and 2086 (57%) in cluster 2. The patients included in cluster 2 were older and more frequently hypertensive and had a higher prevalence of shock, organ dysfunction, inflammatory biomarkers, and worst respiratory indexes at both time points. The 90-day mortality was higher in cluster 2 at both clustering processes (43.8% [n = 1025] versus 27.3% [n = 383] at baseline, and 49% [n = 1023] versus 20.6% [n = 321] on day 3). Four hundred and fifty-eight (33%) patients clustered in the first group were clustered in the second group on day 3. In contrast, 638 (27%) patients clustered in the second group were clustered in the first group on day 3.Conclusions During the first days, patients can be clustered into two groups and the process of clustering patients may change as they continue to evolve. This means that despite a vast majority of patients remaining in the same cluster, a minority reaching 33% of patients analyzed may be re-categorized into different clusters based on their progress. Such changes can significantly impact their prognosis
The evolution of the ventilatory ratio is a prognostic factor in mechanically ventilated COVID-19 ARDS patients
Background: Mortality due to COVID-19 is high, especially in patients requiring mechanical ventilation. The purpose of the study is to investigate associations between mortality and variables measured during the first three days of mechanical ventilation in patients with COVID-19 intubated at ICU admission. Methods: Multicenter, observational, cohort study includes consecutive patients with COVID-19 admitted to 44 Spanish ICUs between February 25 and July 31, 2020, who required intubation at ICU admission and mechanical ventilation for more than three days. We collected demographic and clinical data prior to admission; information about clinical evolution at days 1 and 3 of mechanical ventilation; and outcomes. Results: Of the 2,095 patients with COVID-19 admitted to the ICU, 1,118 (53.3%) were intubated at day 1 and remained under mechanical ventilation at day three. From days 1 to 3, PaO2/FiO2 increased from 115.6 [80.0-171.2] to 180.0 [135.4-227.9] mmHg and the ventilatory ratio from 1.73 [1.33-2.25] to 1.96 [1.61-2.40]. In-hospital mortality was 38.7%. A higher increase between ICU admission and day 3 in the ventilatory ratio (OR 1.04 [CI 1.01-1.07], p = 0.030) and creatinine levels (OR 1.05 [CI 1.01-1.09], p = 0.005) and a lower increase in platelet counts (OR 0.96 [CI 0.93-1.00], p = 0.037) were independently associated with a higher risk of death. No association between mortality and the PaO2/FiO2 variation was observed (OR 0.99 [CI 0.95 to 1.02], p = 0.47). Conclusions: Higher ventilatory ratio and its increase at day 3 is associated with mortality in patients with COVID-19 receiving mechanical ventilation at ICU admission. No association was found in the PaO2/FiO2 variation
Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality
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