Multiplicação de brotos in vitro em três cultivares de bananeira.

Apesar de ser considerada uma das plantas mais consumidas no Brasil, a produtividade média da banana no país é de 10 t/ ha/ ano, sendo que algumas doenças se constituem os principais fatores por esse baixo rendimento. A utilização de mudas de cultivares melhoras e livres de doenças é uma alternativa para aumentar a produtividade Para isso, a técnica de micropropagação tem se mostrado eficaz para a obtenção de mudas sadias e de qualidade e auxiliado os programas de melhoramento genético para a multiplicação rápida de genótipos selecionados. Visando a multiplicação rápida de três cultivares via micropropagação foi realizada a assepsia de ápices caulinares e inoculados em meio MS, com BAP 2,5 mg/L no estabelecimento e BAP 4,5 mg/L durante vários subcultivos. Os resultados mostraram que houve uma produtividade média de 2,03 brotos por ápice caulinar entre as cultivares, e a cultivar PV 0376 apresentou maior, multiplicação de brotos em relação as outras cultivares, média de 2,26 brotos, seguidas de 2,10 e 1,75 nas cultivares Pacovan ken e Thap maeo, respectivamente

Rubella in Sub-Saharan Africa and sensorineural hearing loss: a case control study

Rubella infection can affect several organs and cause birth defects that are responsible for congenital rubella syndrome (CRS). Congenital hearing loss is the most common symptom of this syndrome, occurring in approximately 60% of CRS cases. Worldwide, over 100 000 babies are born with CRS every year. There is no specific treatment for rubella, but the disease is preventable by vaccination. Since 1969, the rubella vaccine has been implemented in many countries, but in Africa, only a few countries routinely immunize against rubella. The aim of this study was to estimate the rate of infection from the wild-type rubella virus in São Tomé and Príncipe by determining rubella seroprevalence with a DBS method. The goal of this study was to reinforce the need for implementation of the rubella vaccine in this country. As secondary objectives, the validation of a DBS method was first attempted and an association between seroprevalence and hearing loss was assessed.info:eu-repo/semantics/publishedVersio

Brigatinib Versus Crizotinib in ALK Inhibitor–Naive Advanced ALK-Positive NSCLC: Final Results of Phase 3 ALTA-1L Trial

Introduction: In the phase 3 study entitled ALK in Lung cancer Trial of brigAtinib in 1st Line (ALTA-1L), which is a study of brigatinib in ALK inhibitor–naive advanced ALK-positive NSCLC, brigatinib exhibited superior progression-free survival (PFS) versus crizotinib in the two planned interim analyses. Here, we report the final efficacy, safety, and exploratory results. Methods: Patients were randomized to brigatinib 180 mg once daily (7-d lead-in at 90 mg once daily) or crizotinib 250 mg twice daily. The primary end point was a blinded independent review committee–assessed PFS. Genetic alterations in plasma cell-free DNA were assessed in relation to clinical efficacy. Results: A total of 275 patients were enrolled (brigatinib, n = 137; crizotinib, n = 138). At study end, (brigatinib median follow-up = 40.4 mo), the 3-year PFS by blinded independent review committee was 43% (brigatinib) versus 19% (crizotinib; median = 24.0 versus 11.1 mo, hazard ratio [HR] = 0.48, 95% confidence interval [CI]: 0.35–0.66). The median overall survival was not reached in either group (HR = 0.81, 95% CI: 0.53–1.22). Posthoc analyses suggested an overall survival benefit for brigatinib in patients with baseline brain metastases (HR = 0.43, 95% CI: 0.21–0.89). Detectable baseline EML4-ALK fusion variant 3 and TP53 mutation in plasma were associated with poor PFS. Brigatinib exhibited superior efficacy compared with crizotinib regardless of EML4-ALK variant and TP53 mutation. Emerging secondary ALK mutations were rare in patients progressing on brigatinib. No new safety signals were observed. Conclusions: In the ALTA-1L final analysis, with longer follow-up, brigatinib continued to exhibit superior efficacy and tolerability versus crizotinib in patients with or without poor prognostic biomarkers. The suggested survival benefit with brigatinib in patients with brain metastases warrants future study

The structure of Herpesvirus Fusion Glycoprotein B-Bilayer Complex reveals the protein-membrane and lateral protein-protein interaction

Glycoprotein B (gB) is a key component of the complex herpesvirus fusion machinery. We studied membrane interaction of two gB ectodomain forms and present an electron cryotomography structure of the gB-bilayer complex. The two forms differed in presence or absence of the membrane proximal region (MPR) but showed an overall similar trimeric shape. The presence of the MPR impeded interaction with liposomes. In contrast, the MPR-lacking form interacted efficiently with liposomes. Lateral interaction resulted in coat formation on the membranes. The structure revealed that interaction of gB with membranes was mediated by the fusion loops and limited to the outer membrane leaflet. The observed intrinsic propensity of gB to cluster on membranes indicates an additional role of gB in driving the fusion process forward beyond the transient fusion pore opening and subsequently leading to fusion pore expansion

Transmural Remission Improves Clinical Outcomes Up to 5 years in Crohn's Disease

Introduction: Evidence supporting transmural remission (TR) as a long-term treatment target in Crohn's disease (CD) is still unavailable. Less stringent but more reachable targets such as isolated endoscopic (IER) or radiologic remission (IRR) may also be acceptable options in the long-term. Methods: Multicenter retrospective study including 404 CD patients evaluated by magnetic resonance enterography and colonoscopy. Five-year rates of hospitalization, surgery, use of steroids, and treatment escalation were compared between patients with TR, IER, IRR, and no remission (NR). Results: 20.8% of CD patients presented TR, 23.3% IER, 13.6% IRR and 42.3% NR. TR was associated with lower risk of hospitalization (odds-ratio [OR] 0.244 [0.111-0.538], p < 0.001), surgery (OR 0.132 [0.030-0.585], p = 0.008), steroid use (OR 0.283 [0.159-0.505], p < 0.001), and treatment escalation (OR 0.088 [0.044-0.176], p < 0.001) compared to no NR. IRR resulted in lower risk of hospitalization (OR 0.333 [0.143-0.777], p = 0.011) and treatment escalation (OR 0.260 [0.125-0.540], p < 0.001), while IER reduced the risk of steroid use (OR 0.442 [0.262-0.745], p = 0.002) and treatment escalation (OR 0.490 [0.259-0.925], p = 0.028) compared to NR. Conclusions: TR improved clinical outcomes over 5 years of follow-up in CD patients. Distinct but significant benefits were seen with IER and IRR. This suggests that both endoscopic and radiologic remission should be part of the treatment targets of CD.info:eu-repo/semantics/publishedVersio

Avaliação de parâmetros fisiológicos de caprinos da raça Anglonubiana em Teresina, Piauí.

O objetivo da pesquisa foi avalia como respostas fisiológicas de cabras em lactação e cabras nuliparas da raça Anglonubiana na Temperatura retal (TR), frequência respiratória (FR) e frequência cardíaca (FC), como variáveis ambientais, temperatura do ar (TA) e umidade do ar (UA), e ainda, temperatura superficial do corpo dos animais e temperatura da pele (TPELE), que foram correlacionados com o peso corporal (PC) e com o escore corporal (EC), em dois períodos do ano (chuvoso e seco). Foram utilizadas 10 cabras lactantes e 10 não lactantes. Foi utilizado o Delineamento em Blocos Casulizados (DBC), ou teste de dados de médias usados para o teste de Tukey. Houve efeito (P0,05) para TR entre as cabras lactentes e não lactantes nos períodos, Pele respectivamente, com efeito (P<0,05) nos períodos. A temperatura do úbere (TÚBERE) com diferença (P<0,05) antes e depois da amamentação (36,95°C). Como correlação das temperaturas de úbere e da veia mamaria foram positivas com todas as demais temperaturas corpóreas. Não foram encontradas, nessa pesquisa, por meio dos parâmetros fisiológicos, as diferenças significativas entre as temperaturas de cabras em fase de produção de leite e cabras nulíparas, nos períodos seco e chuvoso na cidade de Teresina.Título em inglês: Evaluation of physiological parameters of Anglonubian goats in Teresina, Piauí

Avaliação de parâmetros fisiológicos em diferentes raças de caprinos na Região Nordeste brasileira.

Com o crescente aumento da necessidade de produtos de origem animal, vem se buscando alternativas para melhorar o índice produtivo nos rebanhos de caprinos, nessa virtude animais adaptados as condições climáticas vêm se destacando no cesário produtivo, de tal forma que esse pequenos ruminantes possam recorrer a seu mecanismos de termotolerância para manter a temperatura corpórea estável. Para que caprinos venham a desenvolver todo o seu potencial produtivo é necessário que as forças do ambiente, manejo e sanidade estejam a seu favor, nesse sentido inúmeros pesquisadores vêm buscando soluções para que esses requisitos venha a se desenvolver com êxito. Desta forma, o objetivo desta revisão é de reunir informações a respeito do conforto térmico de caprinos com algumas características fisiológicas e comportamentais, bem como, averiguar o efeito do ambiente sobre algumas dessas características.Título em inglês: Evaluation of physiological parameters in different breeds of goats in the Brazilian Northeast Region

Modeling morphological instabilities in lipid membranes with anchored amphiphilic polymers

Anchoring molecules, like amphiphilic polymers, are able to dynamically regulate membrane morphology. Such molecules insert their hydrophobic groups into the bilayer, generating a local membrane curvature. In order to minimize the elastic energy penalty, a dynamic shape instability may occur, as in the case of the curvature-driven pearling instability or the polymer-induced tubulation of lipid vesicles. We review recent works on modeling of such instabilities by means of a mesoscopic dynamic model of the phase-field kind, which take into account the bending energy of lipid bilayers

Functional activities of Bacillus isolated from bromeliads and growth promoting activity of maize exposed to drought stress.

The objective of this work was to caracterize Bacillus spp. isolates regarding their enzymatic properties and the effect of inoculation oh these isolation on germination maize under drougth stress.Sessio 6, Poster VI.8