Improved Temporal Stability of Polymethine Laser Dyes in Aqueous Solutions

Polymethine laser dyes which fluoresce in the near infrared in organic solvents generally exhibit poor temporal stability at room temperature. Two typical laser-pumped polymethine dyes in aqueous solutions containing nonionic surfactants are investigated. Temporal stability was improved 20-fold, as compared to organic solutions, with no significant changes in laser-pumping threshold or tuning range

Interpreting Photoactive Fluorescence Microscopy Measurements of Steady-State Actin Dynamics

Biophysical Journal, 1995A continuum model describing the steady-state actin dynamics of the cytoskeleton of living cells Has been developed to aid in the interpretation of photoactivated fluorescence experiments. In a simplified cell geometry, the model assumes uniform concentrations of cytosolic and cytoskeletal actin throughout the cell and no net growth of either pool. The spatiotemporal evolution of the fluorescent actin population is described by a system of two coupled linear partial-differential equations. An analytical solution is found using a Fourier-Laplace transform and important limiting cases relevant to the design of experiments are discussed. The results demonstrate that, despite being a complex function of the parameters, the fluorescence decay in photoactivated fluorescence experiments has a biphasic behavior featuring a short-term decay controlled by monomer diffusion and a long-term decay governed by the monomer exchange rate between the polymerized and unpolymerized actin pools. This biphasic behavior suggests a convenient mechanism for extracting the parameters governing the fluorescence decay from data records. These parameters include the actin monomer diffusion coefficient, filament turnover rate, and ratio of polymerized to unpolymerized actin

Pressure Dependence of the Infrared Laser Lines in Barium Vapor

We have studied nine laser transitions in Ba vapor at wavelengths between 1.13 and 3.05 micrometer. Three of these laser lines have not been reported previously. The relative intensities of the four strongest transitions (1.13, 1.50, 2.55, and 2.92 micrometer) vary with buffer gas pressure, suggesting that collisional deexcitation of the 6p 1^P_1 state occurs at higher pressure and is the main mechanism populating the upper states of the 2.55 and 2.33 micrometer lines

FUSE: Towards multi-level functional summarization of protein interaction networks

10.1145/2147805.21478062011 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 20112-1

FUSE: A system for data-driven multi-level functional summarization of protein interaction networks

10.1145/2110363.2110470IHI'12 - Proceedings of the 2nd ACM SIGHIT International Health Informatics Symposium847-85