62 research outputs found
Improved Temporal Stability of Polymethine Laser Dyes in Aqueous Solutions
Polymethine laser dyes which fluoresce in the near infrared in
organic solvents generally exhibit poor temporal stability at room
temperature. Two typical laser-pumped polymethine dyes in aqueous
solutions containing nonionic surfactants are investigated. Temporal
stability was improved 20-fold, as compared to organic solutions, with no
significant changes in laser-pumping threshold or tuning range
Interpreting Photoactive Fluorescence Microscopy Measurements of Steady-State Actin Dynamics
Biophysical Journal, 1995A continuum model describing the steady-state actin dynamics of the cytoskeleton of living cells Has been
developed to aid in the interpretation of photoactivated fluorescence experiments. In a simplified cell geometry, the model
assumes uniform concentrations of cytosolic and cytoskeletal actin throughout the cell and no net growth of either pool. The
spatiotemporal evolution of the fluorescent actin population is described by a system of two coupled linear partial-differential
equations. An analytical solution is found using a Fourier-Laplace transform and important limiting cases relevant to the
design of experiments are discussed. The results demonstrate that, despite being a complex function of the parameters, the
fluorescence decay in photoactivated fluorescence experiments has a biphasic behavior featuring a short-term decay
controlled by monomer diffusion and a long-term decay governed by the monomer exchange rate between the polymerized
and unpolymerized actin pools. This biphasic behavior suggests a convenient mechanism for extracting the parameters
governing the fluorescence decay from data records. These parameters include the actin monomer diffusion coefficient,
filament turnover rate, and ratio of polymerized to unpolymerized actin
Pressure Dependence of the Infrared Laser Lines in Barium Vapor
We have studied nine laser transitions in Ba vapor at wavelengths
between 1.13 and 3.05 micrometer. Three of these laser lines have not
been reported previously. The relative intensities of the four strongest
transitions (1.13, 1.50, 2.55, and 2.92 micrometer) vary with buffer gas pressure,
suggesting that collisional deexcitation of the 6p 1^P_1 state occurs
at higher pressure and is the main mechanism populating the upper
states of the 2.55 and 2.33 micrometer lines
Eichung und Einsatz von Hitzdrahtsonden für stationäre Messungen in Strömungen geringer Dichte
Interpreting photoactivated fluorescence microscopy measurements of steady-state actin dynamics
FUSE: Towards multi-level functional summarization of protein interaction networks
10.1145/2147805.21478062011 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 20112-1
FUSE: A system for data-driven multi-level functional summarization of protein interaction networks
10.1145/2110363.2110470IHI'12 - Proceedings of the 2nd ACM SIGHIT International Health Informatics Symposium847-85
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