An ultrasensitive photoelectrochemical nucleic acid biosensor

A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO electrode. The target nucleic acids were hybridized with immobilized oligonucleotide capture probes on the ITO electrode. A subsequent binding of a photoreporter—a photoactive threading bis-intercalator consisting of two N,N′-bis(3-propyl-imidazole)-1,4,5,8-naphthalene diimides (PIND) linked by a [Formula: see text] (bpy = 2,2′-bipyridine) complex (PIND–Ru–PIND)—allowed for photoelectrochemical detection of the target nucleic acids. The extremely low dissociation rate of the adduct and the highly reversible photoelectrochemical response under visible light illumination (490 nm) make it possible to conduct nucleic acid detection, with a sensitivity enhancement of four orders of magnitude over voltammetry. These results demonstrate for the first time the potential of photoelectrochemical biosensors for PCR-free ultrasensitive detection of nucleic acids

Assessing Evapotranspiration Estimates from the Global Soil Wetness Project Phase 2 (GSWP-2) Simulations

Abstract and PDF report are also available on the MIT Joint Program on the Science and Policy of Global Change website (http://globalchange.mit.edu/).We assess the simulations of global-scale evapotranspiration from the Global Soil Wetness Project Phase 2 (GSWP-2) within a global water-budget framework. The scatter in the GSWP-2 global evapotranspiration estimates from various land surface models can constrain the global, annual water budget fluxes to within ±2.5%, and by using estimates of global precipitation, the residual ocean evaporation estimate falls within the range of other independently derived bulk estimates. However, the GSWP-2 scatter cannot entirely explain the imbalance of the annual fluxes from a modern-era, observationally-based global water budget assessment, and inconsistencies in the magnitude and timing of seasonal variations between the global water budget terms are found. Inter-model inconsistencies in evapotranspiration are largest for high latitude inter-annual variability as well as for inter-seasonal variations in the tropics, and analyses with field-scale data also highlights model disparity at estimating evapotranspiration in high latitude regions. Analyses of the sensitivity simulations that replace uncertain forcings (i.e. radiation, precipitation, and meteorological variables) indicate that global (land) evapotranspiration is slightly more sensitive to precipitation than net radiation perturbations, and the majority of the GSWP-2 models, at a global scale, fall in a marginally moisture-limited evaporative condition. Finally, the range of global evapotranspiration estimates among the models is larger than any bias caused by uncertainties in the GSWP-2 atmospheric forcing, indicating that model structure plays a more important role toward improving global land evaporation estimates (as opposed to improved atmospheric forcing).NASA Energy and Water-cycle Study (NEWS, grant #NNX06AC30A), under the NEWS Science and Integration Team activities

An Optimal Strategy for Accurate Bulge-to-disk Decomposition of Disk Galaxies

The development of two-dimensional (2D) bulge-to-disk decomposition techniques has shown their advantages over traditional one-dimensional (1D) techniques, especially for galaxies with non-axisymmetric features. However, the full potential of 2D techniques has yet to be fully exploited. Secondary morphological features in nearby disk galaxies, such as bars, lenses, rings, disk breaks, and spiral arms, are seldom accounted for in 2D image decompositions, even though some image-fitting codes, such as GALFIT, are capable of handling them. We present detailed, 2D multi-model and multi-component decomposition of high-quality $R$-band images of a representative sample of nearby disk galaxies selected from the Carnegie-Irvine Galaxy Survey, using the latest version of GALFIT. The sample consists of five barred and five unbarred galaxies, spanning Hubble types from S0 to Sc. Traditional 1D decomposition is also presented for comparison. In detailed case studies of the 10 galaxies, we successfully model the secondary morphological features. Through a comparison of best-fit parameters obtained from different input surface brightness models, we identify morphological features that significantly impact bulge measurements. We show that nuclear and inner lenses/rings and disk breaks must be properly taken into account to obtain accurate bulge parameters, whereas outer lenses/rings and spiral arms have a negligible effect. We provide an optimal strategy to measure bulge parameters of typical disk galaxies, as well as prescriptions to estimate realistic uncertainties of them, which will benefit subsequent decomposition of a larger galaxy sample.Comment: 30 pages, 14 figures, published in ApJ; minor typos correcte