Research Notes: Amylase and acid phosphatase genotypes of Glycine max, Glycine soja and Neonotonia wightii

Three amylase loci, Am-1, Am-2 , and Am-3, have been identified by electrophoresis (Gorman and Kiang , 1978) . The activity of amylase at Am-1 and Am-2 is very weak, and that at Am-3 is much str onger. Based on heat lability and chemical reaction, Reiss (1978) concluded Am-1 and Am- 2 represent α - amylase and Am-3 β-amylase

Research Notes : United States : Linkage of electrophoretic loci

In our studies of the inheritance of various electrophoretic variants, we have examined F2 segregation data from many crosses (see Gorman, 1983, for a listing). Many of these crosses were segregating for multiple loci, allowing linkage data to be collected. Table 1 represents a sunnnary of the linkage patterns we have observed between the listed electrophoretic loci (see Gorman and Kiang, 1978; Kiang, 1981; Gorman et al., 1983, concerning the establishment of these loci)

Research Notes : United States : Inheritance of a second leucine amino-peptidase locus and tests of its linkage with other loci

In dry soybean seed, only one leucine amino peptidase (LAP) anodal band was observed by acrylamide slab gel electrophoresis (Gorman et al., 1983). This band gradually declined in intensity and disappeared in all tissues about 10-12 days after germination. Three mobility variants (Rf\u27s 0.59, 0.53,and 0.58) were observed in the band, which was controlled by a single locus (Gor-man et al., 1983; Kiang and Gorman, 1983; Gorman, 1983)

Research Notes : Inheritance of soybean electrophoretic variants

We have been using the technique of slab-gel electrophoresis (see Gorman and Kiang, 1977, 1978; Kiang, 1981; Kiang and Gorman, 1983, for methods) to study genetic diversity in G. max and G. soja. In last year\u27s Soybean Genet-ics Newsletter, we reported the accession-specific zymogram types (zymogram types are equivalent to phenotypes) observed in 253 named cultivars (maturi-ty groups 00-IV) for 15 enzyme systems (Gorman et al., 1982b). We have been studying the genetic basis for the differences between these zymogram types

Research Notes : Electrophoretic classification of the early maturity groups of named soybean cultivars

Over the last several years, our lab has been collecting electrophore-tic data for several enzyme systems in G. max and G. soja. While we have not yet completed analysis of all the available G. max or G. soja accessions, we have completed electrophoretic profiles for most of the named soybean cultivars in the early maturity groups (OOO-IV). We have been interested in using these electrophoretic profiles for cultivar identification, since a fairly complete identification can be made (Gorman and Kiang, 1977; and an article in preparation)

Size Effects in Carbon Nanotubes

The inter-shell spacing of multi-walled carbon nanotubes was determined by analyzing the high resolution transmission electron microscopy images of these nanotubes. For the nanotubes that were studied, the inter-shell spacing ${\hat{d}_{002}}$ is found to range from 0.34 to 0.39 nm, increasing with decreasing tube diameter. A model based on the results from real space image analysis is used to explain the variation in inter-shell spacings obtained from reciprocal space periodicity analysis. The increase in inter-shell spacing with decreased nanotube diameter is attributed to the high curvature, resulting in an increased repulsive force, associated with the decreased diameter of the nanotube shells.Comment: 4 pages. RevTeX. 4 figure

Communications Biophysics

Contains reports on three research projects.United States Air Force (Contract AF19(602)-4112

Inactivation of viruses by coherent excitations with a low power visible femtosecond laser

<p>Abstract</p> <p>Background</p> <p>Resonant microwave absorption has been proposed in the literature to excite the vibrational states of microorganisms in an attempt to destroy them. But it is extremely difficult to transfer microwave excitation energy to the vibrational energy of microorganisms due to severe absorption of water in this spectral range. We demonstrate for the first time that, by using a visible femtosecond laser, it is effective to inactivate viruses such as bacteriophage M13 through impulsive stimulated Raman scattering.</p> <p>Results and discussion</p> <p>By using a very low power (as low as 0.5 nj/pulse) visible femtosecond laser having a wavelength of 425 <it>nm </it>and a pulse width of 100 fs, we show that M13 phages were inactivated when the laser power density was greater than or equal to 50 <it>MW/cm</it>². The inactivation of M13 phages was determined by plaque counts and had been found to depend on the pulse width as well as power density of the excitation laser.</p> <p>Conclusion</p> <p>Our experimental findings lay down the foundation for an innovative new strategy of using a very low power visible femtosecond laser to selectively inactivate viruses and other microorganisms while leaving sensitive materials unharmed by manipulating and controlling with the femtosecond laser system.</p

Communications Biophysics

Contains reports on five research projects.National Science Foundation (Grant G-16526)National Institutes of Health (Grant MH-04737-02)