Reducing Decay Losses in Hardwood Forests and Farm Woodlots

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Ship and Satellite Studies of Mesoscale Circulation and Sperm Whale Habitats in the Northeast Gulf of Mexico During GulfCet II

Eighty-three encounters with sperm whales (Physeter macrocephalus) occurred on two cruises that made expendable bathythermograph + conductivity-temperature-depth surveys of cyclone-anticyclone eddy pairs in the Northeast Gulf of Mexico (NEGOM). In late summer 1996, 41 sightings of sperm whales were made and 10 acoustic contacts were registered. Of these 51 encounters, 90% were in a cyclonic area of lower than average dynamic height offshore that was surveyed from space by near-real-time altimetric sea surface height anomaly and then mapped in high resolution with shipboard measurements or within 100 km of SW Pass of the Mississippi River. In midsummer 1997, 23 sightings and nine acoustic contacts were made. Of these 32 encounters, 81% were in an offshore cyclonic area of lower than average dynamic height or within 100 km of the mouth of the Mississippi River. Time series animation of the 1996 and 1997 altimetric data indicated these cyclones are typically associated with Loop Current excursions into the NEGOM and that the two cyclones we surveyed had spun up 4-6 mo previous to our fieldwork. Although cyclones in the NEGOM are temporally persistent, their geographic location is spatially variable: the cyclone surveyed in 1996 was centered 150-200 km south and east of the Mississippi River delta in water 2-3 km deep, whereas that surveyed in 1997 was centered farther east in water 2-3 km deep over DeSoto Canyon. Sperm whales appear to have affinity for cyclonic eddies because the largest numbers of encounters with sperm whales also shifted east in 1997 compared with 1996

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Contains reports on five research projects.National Institute of Mental Health (Grant 5 PO1 MH-13390-04

Synergistic strategy for multicolor two-photon microscopy: application to the analysis of germinal center reactions in vivo

Simultaneous detection of multiple cellular and molecular players in their native environment, one of the keys to a full understanding of immune processes, remains challenging for in vivo microscopy. Here, we present a synergistic strategy for spectrally multiplexed in vivo imaging composed of (i) triple two-photon excitation using spatiotemporal synchronization of two femtosecond lasers, (ii) a broad set of fluorophores with emission ranging from blue to near infrared, (iii) an effective spectral unmixing algorithm. Using our approach, we simultaneously excite and detect seven fluorophores expressed in distinct cellular and tissue compartments, plus second harmonics generation from collagen fibers in lymph nodes. This enables us to visualize the dynamic interplay of all the central cellular players during germinal center reactions. While current in vivo imaging typically enables recording the dynamics of 4 tissue components at a time, our strategy allows a more comprehensive analysis of cellular dynamics involving 8 single-labeled compartments. It enables to investigate the orchestration of multiple cellular subsets determining tissue function, thus, opening the way for a mechanistic understanding of complex pathophysiologic processes in vivo. In the future, the design of transgenic mice combining a larger spectrum of fluorescent proteins will reveal the full potential of our method

Mississippi River and Sea Surface Height Effects on Oil Slick Migration

Millions of barrels of oil escaped into the Gulf of Mexico (GoM) after the 20 April, 2010 explosion of Deepwater Horizon (DH). Ocean circulation models were used to forecast oil slick migration in the GoM, however such models do not explicitly treat the effects of secondary eddy-slopes or Mississippi River (MR) hydrodynamics. Here we report oil front migration that appears to be driven by sea surface level (SSL) slopes, and identify a previously unreported effect of the MR plume: under conditions of relatively high river discharge and weak winds, a freshwater mound can form around the MR Delta. We performed temporal oil slick position and altimeter analysis, employing both interpolated altimetry data and along-track measurements for coastal applications. The observed freshwater mound appears to have pushed the DH oil slick seaward from the Delta coastline. We provide a physical mechanism for this novel effect of the MR, using a two-layer pressure-driven flow model. Results show how SSL variations can drive a cross-slope migration of surface oil slicks that may reach velocities of order km/day, and confirm a lag time of order 5–10 days between mound formation and slick migration, as observed form the satellite analysis. Incorporating these effects into more complex ocean models will improve forecasts of slick migration for future spills. More generally, large SSL variations at the MR mouth may also affect the dispersal of freshwater, nutrients and sediment associated with the MR plume