Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro

<p>Abstract</p> <p>Objectives</p> <p>Chronic rhinosinusitis (CRS) is a chronic inflammatory disease of the nasal mucosa. Recent studies suggest that <it>S. aureus </it>enterotoxins may play an etiologic role in the development of CRS. Apart from surgery and repeated courses of steroids, macrolide antibiotics have been reported to exert anti-inflammatory effects in CRS. Similar effects have been reported for fluoroquinolones on various cell types. Since these effects have poorly been characterized in CRS, we examined anti-inflammatory effects of ciprofloxacin on human nasal epithelial cells (HNECs).</p> <p>Methods</p> <p>Inflammation was induced in HNECs cultured from nasal turbinate mucosa with supernatants of <it>S. aureus Newman </it>for 12 hours. Subsequently, HNECs were coincubated with <it>S. aureus Newman </it>and ciprofloxacin (1.5 × 10^-5M), clarithromycin (10^-6M) or prednisolone (10^-5M) for another 12 hours. IL-8 synthesis was quantified after 12 and 24 hours by ELISA.</p> <p>Results</p> <p>Stimulation with <it>S. aureus Newman </it>supernatants was associated with an increase of IL-8 synthesis after 12 hours in all experiments. During the second 12 hours, IL-8 synthesis decreased and this effect was independent from any stimulus or inhibitor. However, coincubation of HNECs with ciprofloxacin was associated with a more extensive decrease of IL-8 synthesis. Similarly, addition of clarithromycin was associated with a reduction of IL-8 synthesis although this effect was not significant. Coincubation with prednisolone resulted in a significant reduction of IL-8 levels.</p> <p>Conclusion</p> <p>Ciprofloxacin exerts anti-inflammatory effects in <it>S. aureus Newman </it>driven nasal inflammation. Inhibitory effects were comparable to those of prednisolone and clarithromycin.</p

Catheter Colonization and Abscess Formation Due to Staphylococcus epidermidis with Normal and Small-Colony-Variant Phenotype Is Mouse Strain Dependent

Coagulase-negative staphylococci (CoNS) form a thick, multilayered biofilm on foreign bodies and are a major cause of nosocomial implant-associated infections. Although foreign body infection models are well-established, limited in vivo data are available for CoNS with small-colony-variant (SCV) phenotype described as causative agents in implant-associated infections. Therefore, we investigated the impact of the Staphylococcus epidermidis phenotype on colonization of implanted PVC catheters and abscess formation in three different mouse strains. Following introduction of a catheter subcutaneously in each flank of 8- to 12-week-old inbred C57BL/6JCrl (B6J), outbred Crl:CD1(ICR) (CD-1), and inbred BALB/cAnNCrl (BALB/c) male mice, doses of S. epidermidis O-47 wild type, its hemB mutant with stable SCV phenotype, or its complemented mutant at concentrations of 106 to 109 colony forming units (CFUs) were gently spread onto each catheter. On day 7, mice were sacrificed and the size of the abscesses as well as bacterial colonization was determined. A total of 11,500 CFUs of the complemented mutant adhered to the catheter in BALB/c followed by 9,960 CFUs and 9,900 CFUs from S. epidermidis wild type in BALB/c and CD-1, respectively. SCV colonization was highest in CD-1 with 9,500 CFUs, whereas SCVs were not detected in B6J. The minimum dose that led to colonization or abscess formation in all mouse strains was 107 or 108 CFUs of the normal phenotype, respectively. A minimum dose of 108 or 109 CFU of the hemB mutant with stable SCV phenotype led to colonization only or abscess formation, respectively. The largest abscesses were detected in BALB/c inoculated with wild type bacteria or SCV (64 mm2 vs. 28 mm2). Our results indicate that colonization and abscess formation by different phenotypes of S. epidermidis in a foreign body infection model is most effective in inbred BALB/c followed by outbred CD-1 and inbred B6J mice

Bloodstream Infections Caused by Small-Colony Variants of Coagulase-Negative Staphylococci Following Pacemaker Implantation

Small-colony variants (SCVs) of Staphylococcus aureus cause persistent and relapsing infections. Relatively little is known regarding infections caused by SCVs of coagulase-negative staphylococci. We report two cases of pacemaker electrode infections due to SCVs of Staphylococcus epidermidis and Staphylococcus capitis. Sequence analysis of a portion of the 16S rRNA gene (16S rDNA) confirmed the identity of the staphylococcal species as S. capitis and S. epidermidis. Isolates from cultures of blood obtained over at least a 2-week interval were compared by pulsed-field gel electrophoresis and found to be clonal even though the colony morphology was very different. Analysis for auxotrophism revealed hemin dependencies for all isolated SCVs. The two cases have several clinical and laboratory characteristics (which are also seen with S. aureus SCV infections) and strongly suggest that SCVs of coagulase-negative staphylococci must be actively sought, because they grow very slowly and can be easily misse

Search for associations containing young stars (SACY):II. Chemical abundances of stars in 11 young Associations in the Solar neighborhood

The recently discovered coeval, moving groups of young stellar objects in the solar neighborhood represent invaluable laboratories to study recent star formation and to search for high metallicity stars which can be included in future exo-planet surveys. In this study we derived through an uniform and homogeneous method stellar atmospheric parameters and abundances for iron, silicium and nickel in 63 Post T-Tauri Stars from 11 nearby young associations. We further compare the results with two different pre-main sequence (PMS) and main sequence (MS) star populations. The stellar atmospheric parameters and the abundances presented here were derived using the equivalent width of individual lines in the stellar spectra through the excitation/ionization equilibrium of iron. Moreover, we compared the observed Balmer lines with synthetic profiles calculated for model atmospheres with a different line formation code. We found that the synthetic profiles agree reasonably well with the observed profiles, although the Balmer lines of many stars are substantially filled-in, probably by chromospheric emission. Solar metallicity is found to be a common trend on all the nearby young associations studied. The low abundance dispersion within each association strengthens the idea that the origin of these nearby young associations is related to the nearby Star Forming regions (SFR). Abundances of elements other than iron are consistent with previous results on Main Sequence stars in the solar neighborhood. The chemical characterization of the members of the newly found nearby young associations, performed in this study and intended to proceed in subsequent works, is essential for understanding and testing the context of local star formation and the evolutionary history of the galaxy.Comment: 13 pages, 7 figures, 6 tables, accepted for publication in A&A on 26/03/200

A stellar flare during the transit of the extrasolar planet OGLE-TR-10b

We report a stellar flare occurring during a transit of the exoplanet OGLE-TR-10b, an event not previously reported in the literature. This reduces the observed transit depth, particularly in the u'-band, but flaring could also be significant in other bands and could lead to incorrect planetary parameters. We suggest that OGLE-TR-10a is an active planet-hosting star and has an unusually high X-ray luminosity

Staphylococcus aureus small colony variants show common metabolic features in central metabolism irrespective of the underlying auxotrophism

In addition to the classical phenotype, Staphylococcus aureus may exhibit the small colony-variant (SCV) phenotype, which has been associated with chronic, persistent and/or relapsing infections. SCVs are characterized by common phenotypic features such as slow growth, altered susceptibility to antibiotic agents and pathogenic traits based on increased internalization and intracellular persistence. They show frequently auxotrophiesms mainly based on two different mechanisms: (i) deficiencies in electron transport as shown for menadione- and/or hemin-auxotrophs and (ii) thymidylate biosynthetic-defective SCVs. To get a comprehensive overview of the metabolic differences between both phenotypes, we compared sets of clinically derived menadione-, hemin- and thymidine-auxotrophic SCVs and stable site directed mutants exhibiting the SCV phenotype with their corresponding isogenic parental strains displaying the normal phenotype. Isotopologue profiling and transcriptional analysis of central genes involved in carbon metabolism, revealed large differences between both phenotypes. Labeling experiments with [U-13C6]glucose showed reduced 13C incorporation into aspartate and glutamate from all SCVs irrespective of the underlying auxotrophism. More specifically, these SCVs showed decreased fractions of 13C2-aspartate and glutamate; 13C3-glutamate was not detected at all in the SCVs. In comparison to the patterns in the corresponding experiment with the classical S. aureus phenotype, this indicated a reduced carbon flux via the citric acid cycle in all SCV phenotypes. Indeed, the aconitase-encoding gene (acnA) was found down-regulated in all SCV phenotypes under study. In conclusion, all SCV phenotypes including clinical isolates and site-directed mutants displaying the SCV phenotype were characterized by down-regulation of citric acid cycle activity. The common metabolic features in central carbon metabolism found in all SCVs may explain similar characteristics of the S. aure

A homogeneous spectroscopic analysis of host stars of transiting planets

The analysis of transiting extra-solar planets provides an enormous amount of information about the formation and evolution of planetary systems. A precise knowledge of the host stars is necessary to derive the planetary properties accurately. The properties of the host stars, especially their chemical composition, are also of interest in their own right. Information about planet formation is inferred by, among others, correlations between different parameters such as the orbital period and the metallicity of the host stars. The stellar properties studied should be derived as homogeneously as possible. The present work provides new, uniformly derived parameters for 13 host stars of transiting planets. Effective temperature, surface gravity, microturbulence parameter, and iron abundance were derived from spectra of both high signal-to-noise ratio and high resolution by assuming iron excitation and ionization equilibria. For some stars, the new parameters differ from previous determinations, which is indicative of changes in the planetary radii. A systematic offset in the abundance scale with respect to previous assessments is found for the TrES and HAT objects. Our abundance measurements are remarkably robust in terms of the uncertainties in surface gravities. The iron abundances measured in the present work are supplemented by all previous determinations using the same analysis technique. The distribution of iron abundance then agrees well with the known metal-rich distribution of planet host stars. To facilitate future studies, the spectroscopic results of the current work are supplemented by the findings for other host stars of transiting planets, for a total dataset of 50 objects.Comment: accepted for publication in A&A, 7 pages, 6 figure