2 research outputs found
The hemagglutinin-neuraminidase (HN) gene of Newcastle disease virus strain Italien (ndv Italien): comparison with HNs of other strains and expression by a vaccinia recombinant
SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna
A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected
BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long
open reading frame encodes for a protein of 553 amino acids, with a calculated
molecular weight of 59,153, consisting of twelve cysteine residues and six
potential glycosylation sites. The protein sequence contains a hydrophobic region
at the N-terminus of F1 and a presumptive long transmembrane fragment near the
C-terminus. Comparison of the F proteins from NDV strains Italien and
Australia-Victoria shows that the sequences are very similar, with conservation
of most cysteine residues and of the potential glycosylation sites. The F coding
sequence was inserted into the genome of vaccinia virus under the control of
vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was
demonstrated by indirect immunofluorescence with five anti-F monoclonal
antibodies known to react with conformational epitopes