Microtomography developments on the ANATOMIX beamline at Synchrotron SOLEIL

The new ANATOMIX beamline at Synchrotron SOLEIL is dedicated to hard X-ray full-field tomography techniques. Operating in a range of photon energies from approximately 5 to 50 keV, it offers both parallel-beam projection microtomography and nanotomography using a zone-plate transmission X-ray microscope and thus covers a range of spatial resolution from 20 nm to 20 $\mu$m, expressed in terms of useful pixel size. Here we describe the microtomography instrumentation and its performance.Comment: Paper submitted for publication in the Proceedings of the 15th International Conference on X-Ray Microscopy (XRM 2020), 19--24 July 2020, Taipei, Taiwan, edited by D.-H. Wei and C.-M. Cheng and H.-W. Shiu and T.-H. Chuang, AIP Conf. Pro

THE DETERMINATION OF THE INTENSITTIES OF RAMAN LINES

Author Institution: The Sun Oil CompanyAttention is called to the desirability of obtaining intensity data for Raman lines which would permit quantitative comparisons between laboratories using different apparatus. A simple method for obtaining such data is possible by using the $459 cm^{-1}$ line of $CCl_{4}$ as a reference and by eliminating the spectrometer and detector properties by calibrating with the radiation from an incandescent lamp, standardized as Illuminant A by the National Bureau of Standards

Tomato proteome changes following soil application of Pseudomonas spp.

Two commercial biological products Proradix®Agro (Pseudomonas sp.) and Salavida (P. trivialis) were assayed on young tomato plants under Phytophthora infestans challenge, under greenhouse conditions. The products were water suspended following producer’s instruction and applied to the soil 6 days before the foliar inoculation with P. infestans. Late blight symptoms were observed 4 days after pathogen inoculation. Untreated plants appeared more infected than treated plants, but no statistically significant difference was found between treatments and controls or between the two products. However the application of each product led to a visible plant growth enhancement. A comparative study was carried out in order to highlight proteome differences among treated/untreated and inoculated/non inoculated plants. Total proteins were extracted from leaves collected 48h after pathogen inoculation and analysed by two-dimensional electrophoresis. The bioproduct application followed by the pathogen inoculation led to up/down-regulation of some protein spots which were different from those affected by the pathogen only. Mass-spectrometry analysis will be carried out in order to clarify if the observed modifications concern the activation of defence mechanisms or the observed plant growth promotion