Human serum albumin crystals and method of preparation

Human serum albumin (HSA) crystals are provided in the form of tetragonal plates having the space groups P42(sub 1)2, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by a hanging drop method wherein a precipitant solution containing polyethylene glycol (PEG) and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concentration of reagents, pH and other parameters are controlled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies

Estimation in a modified binomial distribution

Estimation techniques in a modified binomial distribution, developed to describe thunderstorm activity over a small area at Cape Kennedy, Florida, are compared. A compound model is also developed and compared with the original model. The minimum Chi square technique is compared with the maximum likelihood and method of moments techniques. The minimum Chi square technique, although useful in complicated models, compared poorly compared to the other techniques. The maximum likelihood and method of moments were comparable. The compound model fit better in every case based on a likelihood ratio test comparing the compound model with the modified binomial model using maximum likelihood estimators

First Line of Defense: Protecting Youth on Campus

Many institutions recognize the need for a centralized process to address the safety of youth on campus. Legislation, publicized incidents, state and national laws, and research have pushed this topic to the forefront. In an effort to educate, mitigate risk, and create consistency, the implementation of university-wide youth protection policies has become a common practice. While each campus is different, foundational components to these policies can create a cultural shift and educate individuals on their role in reporting abuse and neglect. Virginia Commonwealth University (VCU) launched its youth protection policy, Safety and Protection of Minors, in July 2016. The policy took over two years to create and is now in the second year of implementation. Based on the evolution of this policy, from inception to application, the university has learned that this initiative is a shared responsibility and that leaders play a key role

Contractor Drilling with Pounder II

Project Manager Dr Richard Carter, Institute of Water and Environment, Cranfield University at Silsoe.This report contributes to the findings, implications, and future plans of a project, initiated by Cranfield University (Silsoe, UK) entitled “Private Sector Participation in Low Cost Water Well Drilling”.The project was funded by DFID from July 1998 to June 2001, with additional funding partners (Government of Uganda, DANIDA, SIDA, UNICEF, Water Aid, and an anonymous donor) joining at various stages throughout this three-year period

Chaotic string-capture by black hole

We consider a macroscopic charge-current carrying (cosmic) string in the background of a Schwarzschild black hole. The string is taken to be circular and is allowed to oscillate and to propagate in the direction perpendicular to its plane (that is parallel to the equatorial plane of the black hole). Nurmerical investigations indicate that the system is non-integrable, but the interaction with the gravitational field of the black hole anyway gives rise to various qualitatively simple processes like "adiabatic capture" and "string transmutation".Comment: 13 pages Latex + 3 figures (not included), Nordita 93/55

Fields in Nonaffine Bundles. I. The general bitensorially covariant differentiation procedure

The standard covariant differentiation procedure for fields in vector bundles is generalised so as to be applicable to fields in general nonaffine bundles in which the fibres may have an arbitrary nonlinear structure. In addition to the usual requirement that the base space should be flat or endowed with its own linear connection, and that there should be an ordinary gauge connection on the bundle, it is necessary to require also that there should be an intrinsic, bundle-group invariant connection on the fibre space. The procedure is based on the use of an appropriate primary-field (i.e. section) independent connector that is constructed in terms of the natural fibre-tangent-vector realisation of the gauge connection. The application to gauged harmonic mappings will be described in a following article.Comment: 17 page Latex file with some minor misprint corrections and added color for article originally published in black and whit

Protein crystal growth tray assembly

A protein crystal growth tray assembly includes a tray that has a plurality of individual crystal growth chambers. Each chamber has a movable pedestal which carries a protein crystal growth compartment at an upper end. The several pedestals for each tray assembly are ganged together for concurrent movement so that the solutions in the various pedestal growth compartments can be separated from the solutions in the tray's growth chambers until the experiment is to be activated

Objectual understanding, factivity and belief

Should we regard Jennifer Lackey’s (2007) ‘Creationist Teacher’ as understanding evolution, even though she does not, given her religious convictions, believe its central claims? We think this question raises a range of important and unexplored questions about the relationship between understanding, factivity and belief. Our aim will be to diagnose this case in a principled way, and in doing so, to make some progress toward appreciating what objectual understanding—i.e., understanding a subject matter or body of information—demands of us. Here is the plan. After some ground clearing in §1, §2 outlines and motivates a plausible working model—moderate factivity—for characterising the sense in which objectual understanding should be regarded as factive. §3 shows how the datum that we can understand false theories can, despite initial suggestions to the contrary, be assimilated straightforwardly within the moderate factivity model. §4 highlights how the inverse kind of case to that explored in §3—viz., a variant of Lackey’s creationist teacher case—poses special problems for moderate factivity. With reference to recent work on moral understanding by Hills (2009), §5 proposes a solution to the problem, and §6 attempts to diagnose why it is that we might originally have been led to draw the wrong conclusion

Geographical co-location, social networks and inter-firm marketing co-operation : the case of the salmon industry

This study looks at the factors that influence the development of marketing co-operation among cluster-based firms. It examines data from SMEs operating within the salmon farming industry in two different regions: Scotland and Chile. Analyses indicate that informal social networks help explain the observed relationship between geographical proximity and inter-firm marketing co-operation, especially for firms located in peripheral rural communities. A theoretical model is proposed for further research in the field that, until recently, has been traditionally analysed only by economists. Practical implications are suggested for practitioners and policymaker