Actinoplanes siamensis sp. nov., isolated from soil

A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus Actinoplanes. The 16S rRNA gene sequence analysis also indicated that strain A-T 6646<sup>T</sup> belonged to the genus Actinoplanes, being most closely related to Actinoplanes liguriensis DSM 43865<sup>T</sup> (97.61 %) and Actinoplanes octamycinicus NBRC 14524<sup>T</sup> (97.52 %). The DNA-DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H<sub>4</sub>). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C<sub>16: 0</sub>, anteiso-C<sub>17: 0</sub>, iso-C<sub>15: 0</sub> and anteiso-C<sub>15: 0</sub>. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named Actinoplanes siamensis sp. nov. The type strain is A-T 6646<sup>T</sup> (= BCC 46194<sup>T</sup> = NBRC 109076<sup>T</sup>). 2013 IUM

Planobispora siamensis sp nov., isolated from soil

A novel actinomycete strain, A-T 4600T, which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan containedmeso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genusPlanobispora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genusPlanobisporabut as a putative novel species. DNA–DNA relatedness values that differentiate the isolate from previously described members of the genusPlanobisporawere significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species,Planobisporasiamensissp. nov.; the type strain is A-T 4600T( = BCC 39469T = NBRC 107568T).</jats:p

Planobispora takensis sp. nov., isolated from soil

A novel Gram-stain-positive, filamentous bacterial strain (A-T 7458&lt;sup&gt;T&lt;/sup&gt;) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458&lt;sup&gt;T&lt;/sup&gt;had typical characteristics of members of the genus Planobispora. 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600&lt;sup&gt;T&lt;/sup&gt;(98.5 %), Planobispora rosea JCM 3166&lt;sup&gt;T&lt;/sup&gt;(97.6 %) and Planobispora longispora NBRC 13918&lt;sup&gt;T&lt;/sup&gt;(97.6 %). The DNA-DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H&lt;sub&gt;2&lt;/sub&gt;). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C&lt;sub&gt;17:1&lt;/sub&gt; and C&lt;sub&gt;18:1&lt;/sub&gt;, and saturated C&lt;sub&gt;16:0&lt;/sub&gt; and C&lt;sub&gt;17:0&lt;/sub&gt;. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora, to be named Planobispora takensis sp. nov. The type strain is A-T 7458&lt;sup&gt;T&lt;/sup&gt;(=BCC 48396&lt;sup&gt;T&lt;/sup&gt;=NBRC 109077&lt;sup&gt;T&lt;/sup&gt;). 2014 IUM