Solid-State Microwave Electronics

Contains reports on three research projects.National Aeronautics and Space Administration (Grant NGL-22-009-163)Joint Services Electronics Programs (U.S. Army, U.S. Navy, and U.S. Air Force)under Contract DA28-043-AMC-02536(E

Changes in the facial soft tissue profile after maxillary orthognathic surgery

OBJECTIVES To compare the changes of the soft tissue profile in relation to the displacement of the underlying hard structures in maxillary orthognathic surgery and to contribute to the esthetic prediction of the facial profile after surgical procedures. MATERIALS AND METHODS We analyzed the sagittal changes in the facial soft tissue profile related to surgical changes in skeletal structures after maxillary osteotomy in a retrospective study. The study sample comprised 115 adult patients between the ages of 18-50 years who had undergone maxillary orthognathic surgery and interdisciplinary orthodontic treatment at the Department of Orthodontics, Ludwig-Maximilians University of Munich, Germany. LeFort I osteotomy cases in both maxillary monognathic and bignathic osteotomy procedures were included. All subjects had received rigid fixation. A cephalometric analysis of presurgical and postsurgical cephalograms was performed and the correlations between hard tissue and soft tissue change ratios were evaluated using a bivariate linear regression analysis. A vertical line through the landmark sella (S) perpendicular to the nasion-sella line (NSL) served as the reference plane. RESULTS The subnasale (Sn) followed the A point (A) by 57%, the soft tissue A point (A') followed the A point (A) by 73% and the upper lip, represented by the landmark labrale superius (Ls) followed the upper incisor (Is) by 73%; all three in a linear correlation with a mean prediction error of nearly 2 mm. CONCLUSION The scatterplots show a linear correlation with a wide spread for all three pairs of reference points. The wide spread and the high prediction error of almost 2 mm indicate low predictability of the expected lip position and Sn.ZUSAMMENFASSUNG ZIELE: Die Veränderungen des Weichgewebeprofils in Relation zur Verlagerung der darunter liegenden Hartgewebe durch maxilläre orthognathe Chirurgie zu vergleichen und einen Beitrag zur ästhetischen Prognose des Gesichtsprofils nach chirurgischen Maßnahmen zu leisten. MATERIAL UND METHODE In einer retrospektiven Studie analysierten wir die sagittalen Veränderungen des fazialen Weichgewebeprofils in Beziehung zu den chirurgischen Veränderungen der skelettalen Strukturen nach maxillärer Osteotomie. Die Studienprobe besteht aus 115 erwachsenen Patienten im Alter von 18–50 Jahren, die sich interdisziplinär maxillärer orthognather Chirurgie und kieferorthopädischer Therapie an der Poliklinik für Kieferorthopädie der Ludwig-Maximilians-Universität München unterzogen hatten. LeFort-I-Osteotomie-Fälle sowohl maxillärer monognather als auch bignather Osteotomieverfahren wurden in die Studie aufgenommen. Alle Patienten hatten eine starre Fixierung erhalten. Es wurde eine kephalometrische Analyse von präoperativen und postoperativen Fernröntgenseitenbildern durchgeführt, die Korrelationen zwischen Hart- und Weichgewebeveränderungen wurden mittels einer bivariaten linearen Regressionsanalyse ausgewertet. Als Referenzebene diente eine vertikale Linie durch den Referenzpunkt Sella (S), rechtwinklig zur Nasion-Sella-Linie (NSL). ERGEBNISSE Der Punkt Subnasale (Sn) folgte dem A\hbox-Punkt (A) um 57 %, der Weichgewebe-A-Punkt (A′) folgte dem A-Punkt (A) um 73 %, und die Oberlippe, repräsentiert durch den Referenzpunkt Labrale superius (Ls), folgte dem Inzision superius (Is) um 73 %, alle 3 in einer linearen Korrelation bei einer mittleren Abweichung von fast 2 mm. FAZIT Die Punktdiagramme zeigen eine lineare Korrelation mit einer breiten Streuung aller 3 Referenzpunktpaare. Die breite Streuung und die hohe mittlere Abweichung von fast 2 mm lassen auf eine schwache Vorhersagbarkeit der zu erwartenden Position von Oberlippe und Sn schließen

Human Placental Syncytiotrophoblasts Restrict Toxoplasma gondii Attachment and Replication and Respond to Infection by Producing Immunomodulatory Chemokines

Toxoplasma gondii is a major source of congenital disease worldwide, but the cellular and molecular factors associated with its vertical transmission are largely unknown. In humans, the placenta forms the key interface between the maternal and fetal compartments and forms the primary barrier that restricts the hematogenous spread of microorganisms. Here, we utilized primary human trophoblast (PHT) cells isolated from full-term placentas and human midgestation chorionic villous explants to determine the mechanisms by which human trophoblasts restrict and respond to T. gondii infection. We show that placental syncytiotrophoblasts, multinucleated cells that are in direct contact with maternal blood, restrict T. gondii infection at two distinct stages of the parasite lytic cycle—at the time of attachment and also during intracellular replication. Utilizing comparative transcriptome sequencing (RNA-seq) transcriptional profiling, we also show that human placental trophoblasts from both the second and third trimesters respond uniquely to T. gondii infection compared to trophoblast cell lines, typified by the upregulation of several immunity-related genes. One of the most differentially induced genes was the chemokine CCL22, which relies on the secretion of a parasite effector(s) either during or after invasion for its induction. Collectively, our findings provide new insights into the mechanisms by which the human placenta restricts the vertical transmission of T. gondii at early and late stages of human pregnancy and demonstrate the existence of at least two interferon-independent pathways that restrict T. gondii access to the fetal compartment. IMPORTANCE Toxoplasma gondii is a major source of congenital disease worldwide and must breach the placental barrier to be transmitted from maternal blood to the developing fetus. The events associated with the vertical transmission of T. gondii are largely unknown. Here, we show that primary human syncytiotrophoblasts, the fetus-derived cells that comprise the primary placental barrier, restrict T. gondii infection at two distinct stages of the parasite life cycle and respond to infection by inducing a unique immunomodulatory transcriptional profile. Collectively, our findings provide important insights into the mechanisms by which human syncytiotrophoblasts restrict T. gondii infection at early and late stages of human pregnancy, identify both permissive and resistant human placental cell types, and identify the placenta-enriched signaling pathways induced in response to infection

Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018

All living organisms secrete molecules for intercellular communication. Recent research has revealed that extracellular vesicles (EVs) play an important role in inter-organismal cell-to-cell communication by transporting diverse messenger molecules, including RNA, DNA, lipids and proteins. These discoveries have raised fundamental questions regarding EV biology. How are EVs biosynthesized and loaded with messenger/cargo molecules? How are EVs secreted into the extracellular matrix? What are the EV uptake mechanisms of recipient cells? As EVs are produced by all kind of organisms, from unicellular bacteria and protists, filamentous fungi and oomycetes, to complex multicellular life forms such as plants and animals, basic research in diverse model systems is urgently needed to shed light on the multifaceted biology of EVs and their role in inter-organismal communications. To help catalyse progress in this emerging field, a mini-symposium was held in Munich, Germany in August 2018. This report highlights recent progress and major questions being pursued across a very diverse group of model systems, all united by the question of how EVs contribute to inter-organismal communication

Solid-State Microwave Electronics

Contains research objectives and reports on status of research projects.National Aeronautics and Space Administration (Grant NGR-22-009-163

The GRONORUN study: is a graded training program for novice runners effective in preventing running related injuries? Design of a Randomized Controlled Trial

BACKGROUND: Running is a popular form of recreational exercise. Beside the positive effects of running on health and fitness, the risk of a running related injury has to be considered. The incidence of injuries in runners is high and varies from 30–79%. However, few intervention studies on prevention of running related injuries have been performed and none of these studies involved novice runners. METHODS: GRONORUN (Groningen Novice Running) is a two armed randomized controlled trial, comparing the effects of two different training programs for novice runners on the incidence of running related injuries. Participants are novice runners, who want to train for a four mile running event. The control group will train according a standard 8 week training program. The intervention group will use a more gradual, 13 week training program which is based on "the ten percent training rule". During the thirteen week follow up participants register information on running and RRI's in an internet based running log. The primary outcome measure is RRI. An injury is defined as a musculoskeletal ailment of the lower extremity or back, causing a restriction of running for at least one week. DISCUSSION: The GRONORUN trial is the first randomized controlled trial to study a preventive intervention in novice runners. Many different training programs for novice runners are offered, but none are evidence based

Group analysis of structure equations for stars in radiative and convective equilibrium

It is proposed to use the Lie group theory of symmetries of differential equations to investigate the system of equations describing a static star in a radiative and convective equilibrium. It is shown that the action of an admissible group induces a certain algebraic structure in the set of all solutions, which can be used to find a family of new solutions. We have demonstrated that, in the most general case, the equations admit an infinite parameter group of quasi-homologous transformations. We have found invariants of the symmetries group which correspond to the fundamental relations describing a physical characteristic of the stars such as the Hertzsprung-Russell diagram or the mass-luminosity relation. In this way we can suggest that group invariants have not only purely mathematical sense, but their forms are closely associated with the basic empirical relations.Comment: LaTeX2e, 13page

Ensuring center quality, proper patient selection and fair access to chimeric antigen receptor T-cell therapy: position statement of the Austrian CAR-T Cell Network

Chimeric antigen receptor T cells (CAR-T cells) are a novel form of cellular immunotherapy for patients with hematologic and oncologic malignancies. Known side effects of these approved cellular immunotherapies are cytokine release syndrome, immune-cell associated neurotoxicity syndrome, cytopenias, infections and long-lasting B cell aplasia. Safe administration of CAR-T cell therapy requires thorough patient selection and patient care in qualified CAR-T cell centers

Butyrate down-regulates CD44 transcription and liver colonisation in a highly metastatic human colon carcinoma cell line

Over-expression of the adhesion molecule CD44 and its splice variants, especially CD44v6, is associated with poor prognosis and metastasis. We aimed at regulating the expression of CD44 in the highly metastatic human colon cancer cell line HM7 and thereby affecting its metastatic ability. HM7 cells show constitutive expression of CD44 standard and variants isoforms, which were significantly down-regulated by treatment with butyrate. Butyrate significantly inhibited transcription of the CD44 gene and abolished epidermal growth factor-mediated up-regulation of the reporter gene luciferase subcloned upstream to the CD44 promoter (−1.1 kb) and transfected to HM7 cells. Nuclear proteins from butyrate-treated cells bound to an epidermal growth factor receptor element motif present in the CD44 promoter. Epidermal growth factor receptor element-site directed mutations eliminated the inducibility of the luciferase reporter gene and did not allowed binding of nuclear proteins harvested from butyrate-treated cells. Butyrate induced CD44 gene repression by specifically interacting with an epidermal growth factor receptor element nuclear transcriptional factor. This interaction affects CD44 transcriptional activity vis-à-vis in vivo metastatic ability of HM7 cells. These results provide additional insight into the anticarcinogenic properties of butyrate