49 research outputs found

    Biblioteca portatil de los Padres y Doctores de la Iglesia desde el tiempo de los Apostoles

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    Biblioteca portatil de los padres y doctores de la Iglesia desde el tiempo de los apostoles

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    From Biomedicine to Natural History Research: EST Resources for Ambystomatid Aalamanders

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    BACKGROUND: Establishing genomic resources for closely related species will provide comparative insights that are crucial for understanding diversity and variability at multiple levels of biological organization. We developed ESTs for Mexican axolotl (Ambystoma mexicanum) and Eastern tiger salamander (A. tigrinum tigrinum), species with deep and diverse research histories. RESULTS: Approximately 40,000 quality cDNA sequences were isolated for these species from various tissues, including regenerating limb and tail. These sequences and an existing set of 16,030 cDNA sequences for A. mexicanum were processed to yield 35,413 and 20,599 high quality ESTs for A. mexicanum and A. t. tigrinum, respectively. Because the A. t. tigrinum ESTs were obtained primarily from a normalized library, an approximately equal number of contigs were obtained for each species, with 21,091 unique contigs identified overall. The 10,592 contigs that showed significant similarity to sequences from the human RefSeq database reflected a diverse array of molecular functions and biological processes, with many corresponding to genes expressed during spinal cord injury in rat and fin regeneration in zebrafish. To demonstrate the utility of these EST resources, we searched databases to identify probes for regeneration research, characterized intra- and interspecific nucleotide polymorphism, saturated a human - Ambystoma synteny group with marker loci, and extended PCR primer sets designed for A. mexicanum / A. t. tigrinum orthologues to a related tiger salamander species. CONCLUSIONS: Our study highlights the value of developing resources in traditional model systems where the likelihood of information transfer to multiple, closely related taxa is high, thus simultaneously enabling both laboratory and natural history research

    Performance of Ti/Pt and Nb/BDD anodes for dechlorination of nitric acid and regeneration of silver(II) in a tubular reactor for the treatment of solid wastes in nuclear industry

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    One of the problems frequently encountered in the processing of nuclear fuels is the recovery of plutonium contained in various solid wastes. The difficulty is to make soluble the plutonium present as the refractory oxide PuO2. The dissolution of this oxide in nitric acid solutions is easily performed by means of silver(II) a strong oxidizing agent which is usually electrochemically generated on a platinum anode. However, certain solid residues that must be treated to separate actinides contain important quantities of chloride ions that require after dissolution in nitric acid a preliminary electrochemical step to be removed before introducing Ag(I) for Ag(II) electrogeneration. Research is conducted to find electrocatalytic materials being able to replace massive platinum in view to limit capital costs. In the present work a set-up including a two-compartment tubular reactor with recirculation of electrolytes was tested with anodes made of boron doped diamond coated niobium (Nb/BDD) and platinum coated titanium (Ti/Pt) grids for the removal of chlorides (up to 0.1 M) and for silver(II) regeneration. The study showed that these two anodes are effective for the removal of chlorides contained in 6 M HNO3 solution as gaseous chlorine, without producing the unwanted oxyanions of chlorine. Furthermore, the regeneration rate of silver(II) on Nb/BDD anode is approximately equal to that obtained on Ti/Pt anode for the same hydrodynamic conditions in the tubular reactor. Accordingly, dechlorination as well as silver(II) regeneration can be performed in the same reactor equipped either with a Nb/BDD or a Ti/Pt anode. Besides, the service life of Nb/BDD anodes estimated by accelerated life tests conducted in 6 M HNO3 can be considered as very satisfactory compared to that observed with Ti/Pt anodes

    La rémunération des fonctions non-marchandes de la forêt

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    Cet article fait le point sur l'état de la rémunération du forestier, ou tout du moins sur les compensations diverses pouvant être obtenues aujourd'hui pour les services rendus par la forêt
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